Correlation of physical and genetic maps of human chromosome 16
This project aimed to divide chromosome 16 into approximately 50 intervals of {approximately}2Mb in size by constructing a series of mouse/human somatic cell hybrids each containing a rearranged chromosome 16. Using these hybrids, DNA probes would be regionally mapped by Southern blot or PCR analysis. Preference would be given to mapping probes which demonstrated polymorphisms for which the CEPH panel of families had been typed. This would allow a correlation of the physical and linkage maps of this chromosome. The aims have been substantially achieved. 49 somatic cell hybrids have been constructed which have allowed definition of 46, and potentially 57, different physical intervals on the chromosome. 164 loci have been fully mapped into these intervals. A correlation of the physical and genetic maps of the chromosome is in an advanced stage of preparation. The somatic cell hybrids constructed have been widely distributed to groups working on chromosome 16 and other genome projects.
- Research Organization:
- Adelaide Children's Hospital, North Adelaide (Australia)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER60863
- OSTI ID:
- 5702506
- Report Number(s):
- DOE/ER/60863-3; ON: DE92007547
- Country of Publication:
- United States
- Language:
- English
Similar Records
The CEPH consortium linkage map of human chromosome 16
High density physical mapping of chromosome 3p by hybridization of somatic cell hybrid derived Alu-PCR products