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Title: Modification of sodium and potassium channel kinetics by diethyl ether and studies on sodium channel inactivation in the crayfish giant axon membrane

Abstract

The effects of ether and halothane on membrane currents in the voltage clamped crayfish giant axon membrane were investigated. Concentrations of ether up to 300 mM and of halothane up to 32 mM had no effect on resting potential or leakage conductance. Ether and halothane reduced the size of sodium currents without changing the voltage dependence of the peak currents or their reversal potential. Ether and halothane also produced a reversible, dose-dependent speeding of sodium current decay at all membrane potentials. Ether reduced the time constants for inactivation, and also shifted the midpoint of the steady-state inactivation curve in the hyperpolarizing direction. Potassium currents were smaller with ether present, with no change in the voltage dependence of steady-state currents. The activation of potassium channels was faster with ether present. There was no apparent change in the capacitance of the crayfish giant axon membrane with ether concentrations of up to 100 mM. Experiments on sodium channel inactivation kinetics were performed using 4-aminopyridine to block potassium currents. Sodium currents decayed with a time course generally fit well by a single exponential. The time constant of decay was a steep function of voltage, especially in the negative resistance region of the peak currentmore » vs voltage relation.The time course of inactivation was very similar to that of the decay of the current at the same potential. The measurement of steady-state inactivation curves with different test pulses showed no shifts along the voltage asix. The voltage-dependence of the integral of sodium conductance was measured to test models of sodium channel inactivation in which channels must open before inactivating; the results appear inconsistent with some of the simplest cases of such models.« less

Authors:
 [1]
  1. Univ. of Rochester, NY (United States)
Publication Date:
Research Org.:
Univ. of Rochester, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
5658026
Report Number(s):
UR-3490-1787
DOE Contract Number:  
EY-76-C-02-3490
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ANESTHETICS; BIOLOGICAL EFFECTS; CELL MEMBRANES; ELECTRIC POTENTIAL; PERMEABILITY; ETHYL ETHER; MEMBRANE TRANSPORT; BIOCHEMICAL REACTION KINETICS; INACTIVATION; POTASSIUM COMPOUNDS; SODIUM COMPOUNDS; ANIONS; CRUSTACEANS; EXPERIMENTAL DATA; ISOLATED VALUES; NERVE CELLS; VOLTAGE DROP; ALKALI METAL COMPOUNDS; ANIMAL CELLS; ANIMALS; AQUATIC ORGANISMS; ARTHROPODS; CELL CONSTITUENTS; CHARGED PARTICLES; DATA; DATA FORMS; DRUGS; ETHERS; INFORMATION; INVERTEBRATES; IONS; KINETICS; MEMBRANES; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; REACTION KINETICS; SOMATIC CELLS; 551000* - Physiological Systems; 550200 - Biochemistry

Citation Formats

Bean, Bruce Palmer. Modification of sodium and potassium channel kinetics by diethyl ether and studies on sodium channel inactivation in the crayfish giant axon membrane. United States: N. p., 1979. Web. doi:10.2172/5658026.
Bean, Bruce Palmer. Modification of sodium and potassium channel kinetics by diethyl ether and studies on sodium channel inactivation in the crayfish giant axon membrane. United States. https://doi.org/10.2172/5658026
Bean, Bruce Palmer. 1979. "Modification of sodium and potassium channel kinetics by diethyl ether and studies on sodium channel inactivation in the crayfish giant axon membrane". United States. https://doi.org/10.2172/5658026. https://www.osti.gov/servlets/purl/5658026.
@article{osti_5658026,
title = {Modification of sodium and potassium channel kinetics by diethyl ether and studies on sodium channel inactivation in the crayfish giant axon membrane},
author = {Bean, Bruce Palmer},
abstractNote = {The effects of ether and halothane on membrane currents in the voltage clamped crayfish giant axon membrane were investigated. Concentrations of ether up to 300 mM and of halothane up to 32 mM had no effect on resting potential or leakage conductance. Ether and halothane reduced the size of sodium currents without changing the voltage dependence of the peak currents or their reversal potential. Ether and halothane also produced a reversible, dose-dependent speeding of sodium current decay at all membrane potentials. Ether reduced the time constants for inactivation, and also shifted the midpoint of the steady-state inactivation curve in the hyperpolarizing direction. Potassium currents were smaller with ether present, with no change in the voltage dependence of steady-state currents. The activation of potassium channels was faster with ether present. There was no apparent change in the capacitance of the crayfish giant axon membrane with ether concentrations of up to 100 mM. Experiments on sodium channel inactivation kinetics were performed using 4-aminopyridine to block potassium currents. Sodium currents decayed with a time course generally fit well by a single exponential. The time constant of decay was a steep function of voltage, especially in the negative resistance region of the peak current vs voltage relation.The time course of inactivation was very similar to that of the decay of the current at the same potential. The measurement of steady-state inactivation curves with different test pulses showed no shifts along the voltage asix. The voltage-dependence of the integral of sodium conductance was measured to test models of sodium channel inactivation in which channels must open before inactivating; the results appear inconsistent with some of the simplest cases of such models.},
doi = {10.2172/5658026},
url = {https://www.osti.gov/biblio/5658026}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1979},
month = {Mon Jan 01 00:00:00 EST 1979}
}

Thesis/Dissertation:
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