Oligodendroglial response to ionizing radiation: Dose and dose-rate response
An in vitro system using neuroglia from neonatal rat brain was developed to examine the morphologic, immunocytochemical and biochemical response of oligodendroglia to ionizing radiation. Following acute {gamma}-irradiation at day-in-culture (DIC) 8, oligodendrocyte counts at DIC 14 were 55% to 65% of control values after 2 Gy, and 29% to 36% after 5 Gy. Counts increased to near-normal levels at DIC 21 in the 2 Gy group and to 75% of normal in the 5 Gy group. Myelin basic protein levels (MBP) at DIC 14 were 60% of control values after 2 Gy, and 40% after 5 Gy. At DIC 21, MBP after 2 Gy was 45% greater than that observed at DIC 14, but MBP, as a fraction of age-matched control values, dropped from 60% to 50%. Following 5 Gy, absolute MBP changed little between DIC 14 and DIC 21, but decreased from 40% to 25% of control cultures. The response to split-dose irradiation indicated that nearly all sublethal damage in the oligodendrocyte population (and its precursors) was repaired within 3 h to 4 h. A new compartmental cell model for radiation response in vitro of the oligodendrocyte population is proposed and examined in relation to the potential reaction to radiation injury in the brain.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5482509
- Report Number(s):
- LBL-31631; ON: DE92010307
- Resource Relation:
- Other Information: Thesis (Ph.D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Oligodendroglial response to ionizing radiation: Dose and dose-rate response
A STUDY OF THE EFFECTS OF X-IRRADIATION ON THE SPINAL CORDS OF NEONATAL RATS
Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
BRAIN
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
IONIZING RADIATIONS
MYELIN
PROTEINS
RADIATION DOSES
RATS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DOSES
MAMMALS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture