Small-angle neutron scattering studies from solutions of bovine nasal cartilage proteoglycan

Conference · Thu Aug 01 00:00:00 EDT 1985

OSTI ID:5446726

Patel, A; Stivala, S S; Damle, S P; Gregory, J D; Bunick, G J; Uberbacher, E C

Small-angle neutron scattering, SANS, of the proteoglycan subunit of bovine nasal cartilage in 0.15N LiCl at 25/sup 0/C yielded the radius of gyration, R/sub g/, radius of gyration of the cross-section, R/sub q/, persistence length, a, and the molecular weight, M. The following values were obtained: M = 3.9 x 10/sup 6/, R/sub g/ = 745 A, R/sub q/ = 34.6 A and a = 35.2 A. These values compare favorably with those that were obtained from small angle x-ray scattering, SAXS, of a similar extract. The scattering curve of the proteoglycan subunit in D/sub 2/O showed a characteristic broad peak in the specified angular range similar to that observed from SAXS, thus confirming the polyelectrolyte nature of the proteoglycan. 15 refs., 3 figs., 1 tab. (DT)

• Research Organization: Stevens Inst. of Tech., Hoboken, NJ (USA). Dept. of Chemistry and Chemical Engineering; Rockefeller Univ., New York (USA); Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5446726
• Report Number(s): CONF-850871-7; ON: DE85016935
• Resource Relation: Conference: International conference on neutron scattering, Santa Fe, NM, USA, 19 Aug 1985; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
MUCOPOLYSACCHARIDES
NEUTRON DIFFRACTION
AQUEOUS SOLUTIONS
CARTILAGE
COWS
NOSE
PROTEIN STRUCTURE
AMINES
ANIMAL TISSUES
ANIMALS
BODY
BODY AREAS
CARBOHYDRATES
CATTLE
COHERENT SCATTERING
CONNECTIVE TISSUE
DIFFRACTION
DISPERSIONS
DOMESTIC ANIMALS
FACE
HEAD
MAMMALS
MIXTURES
ORGANIC COMPOUNDS
POLYSACCHARIDES
RESPIRATORY SYSTEM
RUMINANTS
SACCHARIDES
SCATTERING
SOLUTIONS
TISSUES
VERTEBRATES
550200* - Biochemistry
400101 - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures