Determination of somatic mutations in human erythrocytes by cytometry
Flow cytometric assays of human erythrocytes labeled with monoclonal antibodies specific for glycophorin A were used to enumerate variant cells that appear in peripheral blood as a result of somatic gene-loss mutations in erythrocyte precursor cells. The assay was performed on erythrocytes from 10 oncology patients who had received at least one treatment from radiation or mutagenic chemotherapy at least 3 weeks before being assayed. The patients were suffering from many different malignancies (e.g., breast, renal, bone, colon and lung), and were treated with several different mutagenic therapeutics (e.g., cisplatinum, adriamycin, daunomycin, or cyclophosphamide). The frequency of these variant cells is an indication of the amount of mutagenic damage accumulated in the individual's erythropoietic cell population. Comparing these results to HPRT clonogenic assays, we find similar baseline frequencies of somatic mutation as well as similar correlation with mutagenic exposures. 9 refs., 3 figs., 1 tab.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5444983
- Report Number(s):
- UCRL-92883; CONF-8506137-10; ON: DE85016082
- Resource Relation:
- Conference: 4. international conference on environmental mutagens, Stockholm, Sweden, 24 Jun 1985
- Country of Publication:
- United States
- Language:
- English
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CELL FLOW SYSTEMS
USES
SOMATIC MUTATIONS
BIOASSAY
BLOOD GROUPS
CHEMOTHERAPY
ERYTHROCYTES
FLUORESCENCE
GLUCOPROTEINS
MAN
MONOCLONAL ANTIBODIES
RADIOTHERAPY
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
LUMINESCENCE
MAMMALS
MATERIALS
MEDICINE
MUTATIONS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOLOGY
SACCHARIDES
THERAPY
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560151 - Radiation Effects on Animals- Man