Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway

Bolo, N R

doi:10.2172/5313663

Title: Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway

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Abstract

The goal of this work is to investigate the potential for and limitations of in vivo nuclear magnetic resonance (NMR) spectroscopy for quantitation of glucose flux through the pentose phosphate pathway (shunt). Interest in the shunt is motivated by the possibility that its activity may be greatly increased in cancer and in the pathological states of cardiac and cerebral ischemia. The ability to dynamically monitor flux through the pentose shunt can give new knowledge about metabolism in pathological states. {sup 13}C NMR spectroscopy was used to monitor shunt activity by determination of the ratios of ({sup 13}C-4) to ({sup 13}C-5)-glutamate, ({sup 13}C-3) to ({sup 13}C-2)-alanine or ({sup 13}C-3) to ({sup 13}C-2)-lactate produced when ({sup 13}C-2)-glucose is infused. These methods provide measures of the effect of oxidative stresses on shunt activity in systems ranging from cell free enzyme-substrate preparations to cell suspensions and whole animals. In anaerobic cell free preparations, the fraction of glucose flux through the shunt was monitored with a time resolution of 3 minutes. This work predicts the potential for in vivo human studies of pentose phosphate pathway activity based on the mathematical simulation of the {sup 13}C fractional enrichments of C4 and C5-glutamate as a function ofmore »« less

Authors:: Bolo, N R

Publication Date:: Fri Nov 01 00:00:00 EST 1991

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE; DOHHS; USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)

OSTI Identifier:: 5313663

Report Number(s):: LBL-31592
ON: DE92010257; CNN: HL07367

DOE Contract Number:: AC03-76SF00098

Resource Type:: Technical Report

Resource Relation:: Other Information: Thesis (Ph.D.)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BRAIN; BIOCHEMISTRY; GLUCOSE; METABOLISM; PENTOSES; AEROBIC CONDITIONS; ALANINES; CARBON 13; IN VIVO; LACTATES; NEOPLASMS; NUCLEAR MAGNETIC RESONANCE; RATS; YEASTS; ALDEHYDES; AMINO ACIDS; ANIMALS; BODY; CARBOHYDRATES; CARBON ISOTOPES; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CHEMISTRY; DISEASES; EUMYCOTA; EVEN-ODD NUCLEI; FUNGI; HEXOSES; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MICROORGANISMS; MONOSACCHARIDES; NERVOUS SYSTEM; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PLANTS; RESONANCE; RODENTS; SACCHARIDES; STABLE ISOTOPES; VERTEBRATES; 550500* - Metabolism; 550200 - Biochemistry; 665100 - Nuclear Techniques in Condensed Matter Physics - (1992-)

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                    Bolo, N R. Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway.  United States: N. p., 1991. 
        Web.  doi:10.2172/5313663.

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                    Bolo, N R. Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway.  United States.  https://doi.org/10.2172/5313663

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                    Bolo, N R. 1991.  
        "Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway".  United States.  https://doi.org/10.2172/5313663.  https://www.osti.gov/servlets/purl/5313663.

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@article{osti_5313663,

  title        = {Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway},

  author       = {Bolo, N R},

  abstractNote = {The goal of this work is to investigate the potential for and limitations of in vivo nuclear magnetic resonance (NMR) spectroscopy for quantitation of glucose flux through the pentose phosphate pathway (shunt). Interest in the shunt is motivated by the possibility that its activity may be greatly increased in cancer and in the pathological states of cardiac and cerebral ischemia. The ability to dynamically monitor flux through the pentose shunt can give new knowledge about metabolism in pathological states. {sup 13}C NMR spectroscopy was used to monitor shunt activity by determination of the ratios of ({sup 13}C-4) to ({sup 13}C-5)-glutamate, ({sup 13}C-3) to ({sup 13}C-2)-alanine or ({sup 13}C-3) to ({sup 13}C-2)-lactate produced when ({sup 13}C-2)-glucose is infused. These methods provide measures of the effect of oxidative stresses on shunt activity in systems ranging from cell free enzyme-substrate preparations to cell suspensions and whole animals. In anaerobic cell free preparations, the fraction of glucose flux through the shunt was monitored with a time resolution of 3 minutes. This work predicts the potential for in vivo human studies of pentose phosphate pathway activity based on the mathematical simulation of the {sup 13}C fractional enrichments of C4 and C5-glutamate as a function of shunt activity and on the signal-to- noise ratio acquired in {sup 13}C NMR human studies from the current literature.},

  doi          = {10.2172/5313663},

  url          = {https://www.osti.gov/biblio/5313663},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Nov 01 00:00:00 EST 1991},

  month        = {Fri Nov 01 00:00:00 EST 1991}

}

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