The role of high-energy synchrotron radiation in biomedical trace element research
This paper will present the results of an investigation of the distribution of essential elements in the normal hepatic lobule. the liver is the organ responsible for metabolism and storage of most trace elements. Although parenchymal hepatocytes are rather uniform histologically, morphometry, histochemistry, immunohistochemistry, and microdissection with microchemical investigations have revealed marked heterogeneity on a functional and biochemical level. Hepatocytes from the periportal and perivenous zones of the liver parrenchyma differ in oxidative energy metabolism, glucose uptake and output, unreagenesis, biotransformation, bile acid secretion, and palsma protein synthesis and secretion. Although trace elements are intimately involved in the regulation and maintenance of these functions, little is known regarding the heterogeneity of trace element localization of the liver parenchyma. Histochemical techniques for trace elements generally give high spatial resolution, but lack specificity and stoichiometry. Microdissection has been of marginal usefulness for trace element analyses due to the very small size of the dissected parenchyma. The characteristics of the high-energy x-ray microscope provide an effective approach for elucidating the trace element content of these small biological structures or regions. 5 refs., 1 fig., 1 tab.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5238628
- Report Number(s):
- BNL-40761; CONF-8708180-6; ON: DE88006296
- Resource Relation:
- Conference: Symposium on X-ray microscopy, Upton, NY, USA, 31 Aug 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Plutonium- or americium-induced liver tumors and lesions in beagles
Modulation of hepatic biotransformation and biliary excretion of bile acid by age and sinusoidal bile acid load
Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
LIVER
ION MICROPROBE ANALYSIS
X-RAY FLUORESCENCE ANALYSIS
BROMINE
CALCIUM
CHROMIUM
COPPER
IRON
MANGANESE
POTASSIUM
RATS
SELENIUM
SPATIAL RESOLUTION
SYNCHROTRON RADIATION
ZINC
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BODY
BREMSSTRAHLUNG
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ELEMENTS
GLANDS
HALOGENS
MAMMALS
METALS
MICROANALYSIS
NONDESTRUCTIVE ANALYSIS
NONMETALS
ORGANS
RADIATIONS
RESOLUTION
RODENTS
SEMIMETALS
TRANSITION ELEMENTS
VERTEBRATES
X-RAY EMISSION ANALYSIS
550600* - Medicine
400101 - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures