Mechanism and structure of the plant plasma membrane Ca{sup 2+}-ATPase

Conference · Fri Dec 31 00:00:00 EST 1993

OSTI ID:510593

Briskin, D P

Objectives of this project were the following: development of an enriched preparation of the red beet plasma membrane Ca{sup 2+} ATPase in order to develop a procedure for detergent solubilization of the enzyme from the membrane using detergents, resolution by a method which could be upscaled for batch isolation, and then reconstitution into liposomes to allow characterization of Ca{sup 2+} transport by the purified enzyme and; characterization of the reaction mechanism for the coupling of nucleoside triphosphate hydrolysis to Ca{sup 2+} transport as mediated by the plasma membrane Ca{sup 2+} ATPase.

• Research Organization: Illinois Univ., Urbana, IL (United States). Dept. of Agronomy
• Sponsoring Organization: USDOE Office of Energy Research, Washington, DC (United States)
• DOE Contract Number: FG02-91ER20057
• OSTI ID: 510593
• Report Number(s): DOE/ER/20057-T1; CONF-9307119-1; ON: DE97006824; TRN: 97:004736
• Resource Relation: Conference: 1993 joint annual meeting of the American Society of Plant Physiologists (ASPP) and the Canadian Society of Plant Physiologists (CSPP), Minneapolis, MN (United States), 31 Jul - 4 Aug 1993; Other Information: PBD: 1993
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ATP-ASE
ENZYME ACTIVITY
CALCIUM
MEMBRANE TRANSPORT
DETERGENTS
HYDROLYSIS
LIPOSOMES
BEETS
TRACER TECHNIQUES
PHOSPHORUS 32
PHOSPHORYLATION
REACTION INTERMEDIATES