Separation of mutagenic components in synthetic crudes
Mutagenic, basic constituents of a synthetic coal oil and a shale oil were isolated from the crude mixtures. In arriving at an efficient isolation procedure, several liquid chromatographic packing-eluent combinations were tried and the fractions bioassayed to determine the distributions of the mutagenic components. The most effective separation was achieved using a sequential elution scheme with first an alumina-benzene combination followed by a Sephadex LH-20 gel-isopropanol-acetone system. About 75 to 80% of an ether soluble base is eluted with benzene through alumina (activity I). Analysis of this fraction has revealed a wide range of alkyl substituted quinolines and pyridines. Material remaining on the alumina column was eluted with ethanol, dried and placed on the Sephadex column. Isopropanol (approximately 250 ml) and acetone (approximately 600 ml) were used in that order to elute the material quantitatively. About 12% of the ether-soluble base is eluted with the isopropanol while the rest (approximately 10%) is eluted with the acetone. Additional alkyl pyridine compounds are eluted with isopropanol while the acetone fractions are predominantly multi-ring nitrogen heterocyclic compounds, according to mass spectral analyses. Bioassay data show excellent isolation of the mutagenic activities into the acetone fractions. Negligible activity is found in the sum of the other (90% wt) fractions.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5054306
- Report Number(s):
- CONF-780305-14
- Resource Relation:
- Conference: Division of fuel chemistry meeting, Anaheim, CA, USA, 12 Mar 1978
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
04 OIL SHALES AND TAR SANDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COAL LIQUIDS
MUTAGENS
CHEMICAL COMPOSITION
SHALE OIL
ACETONE
BENZENE
COLUMN PACKING
HETEROCYCLIC COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
ORGANIC NITROGEN COMPOUNDS
PROPANOLS
PYRIDINES
QUINOLINES
SEPARATION PROCESSES
SYNTHETIC PETROLEUM
ALCOHOLS
AROMATICS
AZINES
CHROMATOGRAPHY
ENERGY SOURCES
FOSSIL FUELS
FUELS
HYDROCARBONS
HYDROXY COMPOUNDS
KETONES
MINERAL OILS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PACKINGS
SPECTROSCOPY
SYNTHETIC FUELS
010600* - Coal
Lignite
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040500 - Oil Shales & Tar Sands- Properties & Composition
016000 - Coal
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040700 - Oil Shales & Tar Sands- Health & Safety
560300 - Chemicals Metabolism & Toxicology