Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice

Conference · Tue Jan 01 00:00:00 EST 1980

OSTI ID:5004543

Stoner, R D; Drew, R T; Bernstein, D M

The effects of inhaled benzene on primary and secondary antibody responses and the incidence of leukemia in mice are reported. Young adult mice were given 5, 12, or 22 exposures to 400 ppM benzene for 6 hrs/day 5 days/week. After the exposure periods, the mice were immunized with absorbed tetanus toxoid (APTT) and/or fluid tetanus toxid (FTT). Exposure to benzene increasingly suppressed primary antibody responses to both antigens. Secondary antibody responses to FTT were nearly normal in animals given 10, 15, or 20 exposures to 400 ppM benzene. Other groups of mice were exposed to either 200 ppM or 50 ppM benzene. Primary antibody responses elicited with FTT and/or APTT were nearly normal in all mice exposed to 50 ppM benzene and in mice exposed to 200 ppM benzene for 5 days. However, 10 and 20 exposures to 200 ppM benzene inhibited antibody production. The effects of chronically inhaled 300 ppM benzene on the time of onset and incidence of leukemia in 400 7-month-old female HRS/J mice were also studied. Two genotypes were used; the (hr/hr) hairless mice are leukemia-prone, whereas the (hr/+) haired mice are more resistant to leukemia. The exposure continued for a period of 6 months. Lymphoid, myeloid, and mixed (lymphoid and myeloid) leukemias were observed. Ninety percent of the (hr/hr) mice exposed to benzene died from leukemia as compared with 91% for the (hr/hr) air control group. Eighty-five percent of the (hr/+) mice exposed to benzene died from leukemia as compared with 81% for the (hr/+) air control group. Exposures to 300 ppM benzene did not alter the time of onset or the incidence of leukemia commonly expected in HRS/J mice.

• Research Organization: Brookhaven National Lab., Upton, NY (USA)
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5004543
• Report Number(s): BNL-28428; CONF-801039-2
• Resource Relation: Conference: 20. annual Hanford Life Sciences symposium, Richland, WA, USA, 19 Oct 1980
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZENE
BIOLOGICAL EFFECTS
LEUKEMOGENESIS
IMMUNE REACTIONS
DOSE-RESPONSE RELATIONSHIPS
IMMUNITY
PATHOLOGICAL CHANGES
LEUKEMIA
GENETIC VARIABILITY
MICE
SENSITIVITY
ADULTS
ANTIBODY FORMATION
CHRONIC EXPOSURE
EXPERIMENTAL DATA
EXPOSURE CHAMBERS
FEMALES
GENOTYPE
INHALATION
TETANUS
TOXICITY
TOXINS
AGE GROUPS
ANIMALS
ANTIGENS
AROMATICS
BIOLOGICAL VARIABILITY
CARCINOGENESIS
DATA
DISEASES
HEMIC DISEASES
HYDROCARBONS
INFECTIOUS DISEASES
INFORMATION
INTAKE
MAMMALS
MATERIALS
NEOPLASMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOGENESIS
RODENTS
TOXIC MATERIALS
VERTEBRATES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
551000 - Physiological Systems
550900 - Pathology