An investigation concerning the validation of thin substrate film techniques for the histochemical detection of ribonulease activity: Negative radioautographic localization of ribonucleodepolymerase activities by the use of carbon-14 labeled RNA, tritium labeled RNA, and tritium labeled synthetic polyribonuleotides incorporated into thin substrate films

Thesis/Dissertation · Tue Oct 31 00:00:00 EST 1972

DOI:https://doi.org/10.2172/4721458· OSTI ID:4721458

Woolfrey, Bertram F. ^[1]

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1. Univ. of Minnesota, Minneapolis, MN (United States)

In the foregoing portions of this thesis, the substrate film technique of Daoust has been studied with respect to the pertinent world literature and by means of certain new experimental approaches. A consideration of the literature indicates that many problems are potentially inherent in substrate definition.

• Research Organization: Univ. of Minnesota, Minneapolis, MN (United States)
• DOE Contract Number: AT(11-1)-1089
• NSA Number: NSA-26-015553
• OSTI ID: 4721458
• Report Number(s): COO-1089-16
• Resource Relation: Other Information: Thesis. UNCL. Orig. Receipt Date: 31-DEC-72
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
36 MATERIALS SCIENCE
AUTORADIOGRAPHY
BIOCHEMISTRY
CARBON 14
IN VITRO
LABELLED COMPOUNDS
NUCLEOTIDES
RNA
RNA-ASE
SAMPLING
TESTING
TRACER TECHNIQUES
TRANSFERASES
TRITIUM
USES
TRANSFERASES/histochemical localization of activity of ribonuclease
method using radioisotope labeled thin substrate films and autoradiography