Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters
Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.
- Research Organization:
- BDM Oklahoma, Inc., Bartlesville, OK (United States)
- OSTI ID:
- 270508
- Report Number(s):
- CONF-9509173-; ON: DE96001220; TRN: 96:002616-0036
- Resource Relation:
- Conference: International conference on microbial enhanced oil recovery and related biotechnology for solving environment problems, Dallas, TX (United States), 11-14 Sep 1995; Other Information: PBD: [1995]; Related Information: Is Part Of The fifth international conference on microbial enhanced oil recovery and related biotechnology for solving environmental problems: 1995 Conference proceedings; Bryant, R. [ed.]; PB: 619 p.
- Country of Publication:
- United States
- Language:
- English
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