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Title: Modified bacterial microcompartment shell proteins

Patent ·
OSTI ID:1840488
; ; ; ;

The present invention provides for a fusion protein comprising (1) a bacterial microcompartment (BMC) shell protein comprising one or more subunit, and (2) a first component of a specific-binding pair, operably linked to the BMC shell protein such that the first component faces (i) a lumen (inside) side, or (ii) outside of a BMC shell formed incorporating the fusion protein and the fusion protein does not disrupt or prevent the folding of the BMC shell protein or the ability of the BMC shell protein to integrate with other BMC shell proteins into a BMC shell; wherein the first component is capable of forming a stable or irreversible interaction with a second component of the specific-binding pair.

Research Organization:
University of California, Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (Unites States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE; National Institutes of Health (NIH)
DOE Contract Number:
1R01AI114975-01; AC02-05CH11231; FG02-91ER20021
Assignee:
The Regents of the University of California (Oakland, CA); Board of Trustees of Michigan State University (East Lansing, MI)
Patent Number(s):
11,130,788
Application Number:
16/412,133
OSTI ID:
1840488
Resource Relation:
Patent File Date: 05/14/2019
Country of Publication:
United States
Language:
English

References (46)

Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway journal November 2013
A Simple Tagging System for Protein Encapsulation journal April 2006
Protein Structures Forming the Shell of Primitive Bacterial Organelles journal August 2005
The Pentameric Vertex Proteins Are Necessary for the Icosahedral Carboxysome Shell to Function as a CO2 Leakage Barrier journal October 2009
Foldit Standalone: a video game-derived protein structure manipulation interface using Rosetta journal May 2017
BglBrick vectors and datasheets: A synthetic biology platform for gene expression journal January 2011
Cyan and Yellow Super Fluorescent Proteins with Improved Brightness, Protein Folding, and FRET Förster Radius † , ‡ journal May 2006
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells journal July 1998
Design and Implementation of Novel and/or Enhanced Bacterial Microcompartments for Customizing Metabolis patent-application August 2012
Constructs for Expressing Biological Molecules that Integrate Into Bacterial Microcompartments patent-application August 2016
Atomic-Level Models of the Bacterial Carboxysome Shell journal February 2008
Minimized Cyanobacterial Microcompartment for Carbon Dioxide Fixation patent-application March 2018
Elucidating Essential Role of Conserved Carboxysomal Protein CcmN Reveals Common Feature of Bacterial Microcompartment Assembly journal March 2012
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93% journal January 2012
De novo design of signal sequences to localize cargo to the 1,2-propanediol utilization microcompartment : Design of Signal Sequences journal April 2017
Crystal structures of β-carboxysome shell protein CcmP: ligand binding correlates with the closed or open central pore journal March 2017
A synthetic system for expression of components of a bacterial microcompartment journal September 2013
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method journal October 2014
Identification and Structural Analysis of a Novel Carboxysome Shell Protein with Implications for Metabolite Transport journal September 2009
The Carboxysome Shell Is Permeable to Protons journal September 2010
Engineered Protein Nano-Compartments for Targeted Enzyme Localization journal March 2012
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin journal February 2012
Engineered Subcellular Compartments patent-application October 2014
Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag journal January 2014
Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis journal March 2008
Assembly of Robust Bacterial Microcompartment Shells Using Building Blocks from an Organelle of Unknown Function journal May 2014
Evidence for Improved Encapsulated Pathway Behavior in a Bacterial Microcompartment through Shell Protein Engineering journal June 2017
Enzymatic assembly of DNA molecules up to several hundred kilobases journal April 2009
Engineering Bacterial Microcompartment Shells: Chimeric Shell Proteins and Chimeric Carboxysome Shells journal July 2014
Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell journal June 2017
The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle journal March 2016
Microcompartments for B12-Dependent 1,2-Propanediol Degradation Provide Protection from DNA and Cellular Damage by a Reactive Metabolic Intermediate journal February 2008
Constructs and Systems and Methods for Producing Microcompartments patent-application January 2015
Molecular genetic analysis of a plant virus polyprotein cleavage site: A model journal August 1989
Isolation and Characterization of High CO 2 -Requiring-Mutants of the Cyanobacterium Synechococcus PCC7942 : Two Phenotypes that Accumulate Inorganic Carbon but Are Apparently Unable to Generate CO journal October 1989
UCSF Chimera?A visualization system for exploratory research and analysis journal January 2004
Evolution of Coenzyme B12 Synthesis Among Enteric Bacteria: Evidence for Loss and Reacquisition of a Multigene Complex journal January 1996
Expression and Purification of Soluble His6-Tagged TEV Protease book January 2009
Bacterial microcompartment assembly: The key role of encapsulation peptides journal May 2015
Methods for Producing Microcompartments patent-application April 2017
The Structure of CcmP, a Tandem Bacterial Microcompartment Domain Protein from the β-Carboxysome, Forms a Subcompartment Within a Microcompartment journal April 2013
A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment journal September 2016
Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds patent January 2017
A systems-level model reveals that 1,2-Propanediol utilization microcompartments enhance pathway flux through intermediate sequestration journal May 2017
Targeting Signal for Integrating Proteins, Peptides and Biological Molecules Into Bacterial Microcompartments patent-application May 2013
Short N-terminal sequences package proteins into bacterial microcompartments journal March 2010

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