Host cells and methods for producing toluene biochemically

Beller, Harry R.; Rodrigues, Andria V.; Zargar, Kamrun

Title: Host cells and methods for producing toluene biochemically

Patent · Tue Aug 03 00:00:00 EDT 2021

OSTI ID:1840306

Beller, Harry R.; Rodrigues, Andria V.; Zargar, Kamrun

The present invention provides for a genetically modified host cell comprising a first polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase, and having an enzymatic activity to decarboxylate a phenylacetic acid into a toluene and a carbon dioxide, and a second polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase activating enzyme, and having an enzymatic activity to cleave a S-adenosylmethionine (SAM) to form a methionine and a 5′-deoxyadenosyl radical.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,078,499

Application Number:: 16/286,411

OSTI ID:: 1840306

Resource Relation:: Patent File Date: 02/26/2019

Country of Publication:: United States

Language:: English

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