Lpp positions peptidoglycan at the AcrA-TolC interface in the AcrAB-TolC multidrug efflux pump

Gumbart, James C.; Ferreira, Josie L.; Hwang, Hyea; Hazel, Anthony; Cooper, Connor; Parks, Jerry M.; Smith, Jeremy C.; Zgurskaya, Helen I.; Beeby, Morgan

doi:10.1016/j.bpj.2021.08.016

Title: Lpp positions peptidoglycan at the AcrA-TolC interface in the AcrAB-TolC multidrug efflux pump

Journal Article · Tue Aug 17 00:00:00 EDT 2021 · Biophysical Journal

DOI:https://doi.org/10.1016/j.bpj.2021.08.016· OSTI ID:1822054

Gumbart, James C. ^[1]; Ferreira, Josie L. ^[2]; Hwang, Hyea ^[1]; Hazel, Anthony ^[1]; Cooper, Connor ^[3];

^[3];

^[4]; Zgurskaya, Helen I. ^[5]; Beeby, Morgan ^[2]

Georgia Inst. of Technology, Atlanta, GA (United States)
Imperial College, London (United Kingdom)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Oklahoma, Norman, OK (United States)

The multidrug efflux pumps of Gram-negative bacteria are a class of complexes that span the periplasm, coupling both the inner and outer membranes to expel toxic molecules. The best-characterized example of these tripartite pumps is the AcrAB-TolC complex of Escherichia coli. However, how the complex interacts with the peptidoglycan (PG) cell wall, which is anchored to the outer membrane (OM) by Braun’s lipoprotein (Lpp), is still largely unknown. In this work, we present molecular dynamics simulations of a complete, atomistic model of the AcrAB-TolC complex with the inner membrane, OM, and PG layers all present. We find that the PG localizes to the junction of AcrA and TolC, in agreement with recent cryo-tomography data. Free-energy calculations reveal that the positioning of PG is determined by the length and conformation of multiple Lpp copies anchoring it to the OM. The distance between the PG and OM measured in cryo-electron microscopy images of wild-type E. coli also agrees with the simulation-derived spacing. Finally, sequence analysis of AcrA suggests a conserved role for interactions with PG in the assembly and stabilization of efflux pumps, one that may extend to other trans-envelope complexes as well.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); Medical Research Council

Grant/Contract Number:: AC05-00OR22725; R01-AI052293; MR/P019374/1; MR/K501281/1; ACI-1548562

OSTI ID:: 1822054

Journal Information:: Biophysical Journal, Vol. 120, Issue 18; ISSN 0006-3495

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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