Designed proteins for pH switchable antibody purification

Patent · Tue Mar 23 00:00:00 EDT 2021

OSTI ID:1805652

Jha, Roshan Kumar; Strauss, Charlie E.; Bradbury, Andrew M.

Modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, a modified Fc-binding domain includes or consists of the amino acid sequence of any one of SEQ ID NOs: 4, 6, or 8. Nucleic acids encoding the modified Fc-binding domains or polypeptides including one or more of the modified Fc-binding domains are also provided. In some embodiments, the nucleic acids are included in a vector and may be operably linked to a promoter. Methods for purifying a polypeptide including one or more Fc regions are also provided. Kits and reagents including the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are linked to a solid support.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC52-06NA25396
• Assignee: Triad National Security, LLC (Los Alamos, NM)
• Patent Number(s): 10,954,275
• Application Number: 16/054,882
• OSTI ID: 1805652
• Resource Relation: Patent File Date: 08/03/2018
• Country of Publication: United States
• Language: English