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Title: Frequency-based modulation of diverse species in a nucleic acid library

Patent ·
OSTI ID:1771684
; ; ;

A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Research Organization:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-02ER63445
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Number(s):
10,851,369
Application Number:
16/310,643
OSTI ID:
1771684
Resource Relation:
Patent File Date: 06/21/2017
Country of Publication:
United States
Language:
English

References (6)

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System journal October 2015
Self-targeting Genome Editing System patent-application October 2018
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements journal November 2015
Methods and Compositions for Identification of Highlly Specific Nucleases patent-application January 2018
A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 journal January 2010
Gene Targeting and Genetic Modifcation of Plants Via RNA-Guided Genome Editing patent-application March 2015

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