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Title: IMG/M 4 version of the integrated metagenome comparative analysis system

Journal Article · · Nucleic Acids Research
DOI:https://doi.org/10.1093/nar/gkt919· OSTI ID:1625530
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  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Computational Research Division. Biological Data Management and Technology Center
  2. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States). Microbial Genome and Metagenome Program

IMG/M (http://img.jgi.doe.gov/m) provides support for comparative analysis of microbial community aggregate genomes (metagenomes) in the context of a comprehensive set of reference genomes from all three domains of life, as well as plasmids, viruses and genome fragments. IMG/M’s data content and analytical tools have expanded continuously since its first version was released in 2007. Since the last report published in the 2012 NAR Database Issue, IMG/M’s database architecture, annotation and data integration pipelines and analysis tools have been extended to cope with the rapid growth in the number and size of metagenome data sets handled by the system. IMG/M data marts provide support for the analysis of publicly available genomes, expert review of metagenome annotations (IMG/M ER: http://img.jgi.doe.gov/mer) and Human Microbiome Project (HMP)-specific metagenome samples (IMG/M HMP: http://img.jgi.doe.gov/ imgm_hmp).

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1625530
Journal Information:
Nucleic Acids Research, Vol. 42, Issue D1; ISSN 0305-1048
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

References (22)

IMG 4 version of the integrated microbial genomes comparative analysis system journal October 2013
The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata journal December 2011
The minimum information about a genome sequence (MIGS) specification journal May 2008
A Fast and Symmetric DUST Implementation to Mask Low-Complexity DNA Sequences journal June 2006
CRISPR Recognition Tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats journal June 2007
PILER-CR: Fast and accurate identification of CRISPR repeats journal January 2007
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence journal March 1997
Ab initio gene identification in metagenomic sequences journal April 2010
MetaGene: prokaryotic gene finding from environmental genome shotgun sequences journal October 2006
Prodigal: prokaryotic gene recognition and translation initiation site identification journal March 2010
FragGeneScan: predicting genes in short and error-prone reads journal August 2010
The COG database: an updated version includes eukaryotes journal January 2003
The Pfam protein families database journal November 2011
KEGG for integration and interpretation of large-scale molecular data sets journal November 2011
Search and clustering orders of magnitude faster than BLAST journal August 2010
IMG/M: the integrated metagenome data management and comparative analysis system journal November 2011
A call for standardized classification of metagenome projects: Genomics update journal March 2010
Insights into the phylogeny and coding potential of microbial dark matter journal July 2013
A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea journal December 2009
IMG/M-HMP: A Metagenome Comparative Analysis System for the Human Microbiome Project journal July 2012
Fast gapped-read alignment with Bowtie 2 journal March 2012
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence journal March 1997

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