Transcriptome sequencing of three Pseudo-nitzschia species reveals comparable gene sets and the presence of Nitric Oxide Synthase genes in diatoms

Di Dato, Valeria; Musacchia, Francesco; Petrosino, Giuseppe; Patil, Shrikant; Montresor, Marina; Sanges, Remo; Ferrante, Maria Immacolata

doi:10.1038/srep12329

Title: Transcriptome sequencing of three Pseudo-nitzschia species reveals comparable gene sets and the presence of Nitric Oxide Synthase genes in diatoms

Journal Article · Mon Jul 20 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep12329· OSTI ID:1624783

Di Dato, Valeria ^[1]; Musacchia, Francesco ^[2]; Petrosino, Giuseppe ^[2]; Patil, Shrikant ^[2]; Montresor, Marina ^[2]; Sanges, Remo ^[2]; Ferrante, Maria Immacolata ^[2]

Stazione Zoologica Anton Dohrn, Villa Comunale Naples, (Italy)
Stazione Zoologica Anton Dohrn, Villa Comunale Naples, (Italy)

Diatoms are among the most diverse eukaryotic microorganisms on Earth, they are responsible for a large fraction of primary production in the oceans and can be found in different habitats. Pseudo-nitzschia are marine planktonic diatoms responsible for blooms in coastal and oceanic waters. We analyzed the transcriptome of three species, Pseudo-nitzschia arenysensis, Pseudo-nitzschia delicatissima and Pseudo-nitzschia multistriata, with different levels of genetic relatedness. These species have a worldwide distribution and the last one produces the neurotoxin domoic acid. We were able to annotate about 80% of the sequences in each transcriptome and the analysis of the relative functional annotations allowed comparison of the main metabolic pathways, pathways involved in the biosynthesis of isoprenoids (MAV and MEP pathways) and pathways putatively involved in domoic acid synthesis. The search for homologous transcripts among the target species and other congeneric species resulted in the discovery of a sequence annotated as Nitric Oxide Synthase (NOS), found uniquely in Pseudo-nitzschia multistriata. The predicted protein product contained all the domains of the canonical metazoan sequence. Putative NOS sequences were found in other available diatom datasets, supporting a role for nitric oxide as signaling molecule in this group of microalgae.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1624783

Journal Information:: Scientific Reports, Vol. 5, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 45 works

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