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Title: De novo transcriptome assembly databases for the central nervous system of the medicinal leech

Journal Article · · Scientific Data
 [1];  [2]; ORCiD logo [3];  [2]
  1. Bar Ilan Univ., Ram Gan (Israel). Faculty of Life Sciences; Bar Ilan Univ., Ram Gan (Israel). Faculty of Engineering; Bar Ilan Univ., Ram Gan (Israel). Inst. of Nanotechnologies and Advanced Materials
  2. Bar Ilan Univ., Ram Gan (Israel). Faculty of Engineering; Bar Ilan Univ., Ram Gan (Israel). Inst. of Nanotechnologies and Advanced Materials
  3. Bar Ilan Univ., Ram Gan (Israel). Faculty of Life Sciences
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The study of non-model organisms stands to benefit greatly from genetic and genomic data. For a better understanding of the molecular mechanisms driving neuronal development, and to characterize the entire leech Hirudo medicinalis central nervous system (CNS) transcriptome we combined Trinity for de-novo assembly and Illumina HiSeq2000 for RNA-Seq. We present a set of 73,493 de-novo assembled transcripts for the leech, reconstructed from RNA collected, at a single ganglion resolution, from the CNS. This set of transcripts greatly enriches the available data for the leech. Here, we share two databases, such that each dataset allows a different type of search for candidate homologues. The first is the raw set of assembled transcripts. This set allows a sequence-based search. A comprehensive analysis of which revealed 22,604 contigs with high e-values, aligned versus the Swiss-Prot database. This analysis enabled the production of the second database, which includes correlated sequences to annotated transcript names, with the confidence of BLAST best hit.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1624541
Journal Information:
Scientific Data, Vol. 2, Issue 1; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (6)

Hirudins of the Asian medicinal leech, Hirudinaria manillensis: same same, but different journal June 2019
De novo assembly and comparative transcriptome characterization of Poecilobdella javanica provide insight into blood feeding of medicinal leeches journal January 2018
Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches posted_content September 2019
Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches journal April 2020
An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity journal July 2018
Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential journal October 2016

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