“Candidatus Thermonerobacter thiotrophicus,” A Non-phototrophic Member of the Bacteroidetes/Chlorobi With Dissimilatory Sulfur Metabolism in Hot Spring Mat Communities

Thiel, Vera; Garcia Costas, Amaya M.; Fortney, Nathaniel W.; Martinez, Joval N.; Tank, Marcus; Roden, Eric E.; Boyd, Eric S.; Ward, David M.; Hanada, Satoshi; Bryant, Donald A.

doi:10.3389/fmicb.2018.03159

Title: “Candidatus Thermonerobacter thiotrophicus,” A Non-phototrophic Member of the Bacteroidetes/Chlorobi With Dissimilatory Sulfur Metabolism in Hot Spring Mat Communities

Journal Article · Wed Jan 09 00:00:00 EST 2019 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2018.03159· OSTI ID:1610260

Thiel, Vera ^[1]; Garcia Costas, Amaya M. ^[2]; Fortney, Nathaniel W. ^[3]; Martinez, Joval N. ^[4]; Tank, Marcus ^[1]; Roden, Eric E. ^[3]; Boyd, Eric S. ^[5]; Ward, David M. ^[5]; Hanada, Satoshi ^[6]; Bryant, Donald A. ^[7]

Tokyo Metropolitan Univ. (Japan); Pennsylvania State Univ., University Park, PA (United States)
Pennsylvania State Univ., University Park, PA (United States); Univ. of Colorado, Pueblo, CO (United States)
Univ. of Wisconsin, Madison, WI (United States)
Tokyo Metropolitan Univ. (Japan); Univ. of St. La Salle, Bacolod (Philippines)
Montana State Univ., Bozeman, MT (United States)
Tokyo Metropolitan Univ. (Japan)
Pennsylvania State Univ., University Park, PA (United States); Montana State Univ., Bozeman, MT (United States)

In this study we present evidence for a novel, thermophilic bacterium with dissimilatory sulfur metabolism, tentatively named “Candidatus Thermonerobacter thiotrophicus,” which is affiliated with the Bacteroides/Ignavibacteria/Chlorobi and which we predict to be a sulfate reducer. Dissimilatory sulfate reduction (DSR) is an important and ancient metabolic process for energy conservation with global importance for geochemical sulfur and carbon cycling. Characterized sulfate-reducing microorganisms (SRM) are found in a limited number of bacterial and archaeal phyla. However, based on highly diverse environmental dsrAB sequences, a variety of uncultivated and unidentified SRM must exist. The recent development of high-throughput sequencing methods allows the phylogenetic identification of some of these uncultured SRM. In this study, we identified a novel putative SRM inhabiting hot spring microbial mats that is a member of the OPB56 clade (“Ca. Kapabacteria”) within the Bacteroidetes/Chlorobi superphylum. Partial genomes for this new organism were retrieved from metagenomes from three different hot springs in Yellowstone National Park, United States, and Japan. Supporting the prediction of a sulfate-reducing metabolism for this organism during period of anoxia, diel metatranscriptomic analyses indicate highest relative transcript levels in situ for all DSR-related genes at night. The presence of terminal oxidases, which are transcribed during the day, further suggests that these organisms might also perform aerobic respiration. The relative phylogenetic proximity to the sulfur-oxidizing, chlorophototrophic Chlorobi further raises new questions about the evolution of dissimilatory sulfur metabolism.

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Research Organization:: Pennsylvania State Univ., University Park, PA (United States); Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-94ER20137; AC02-05CH11231

OSTI ID:: 1610260

Journal Information:: Frontiers in Microbiology, Vol. 9; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 29 works

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A Deeply Branching Thermophilic Bacterium with an Ancient Acetyl-CoA Pathway Dominates a Subsurface Ecosystem Takami, Hideto; Noguchi, Hideki; Takaki, Yoshihiro PLoS ONE, Vol. 7, Issue 1 https://doi.org/10.1371/journal.pone.0030559	journal	January 2012
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Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria , and a proposal of a novel bacterial phylum I gnavibacteriae Podosokorskaya, Olga A.; Kadnikov, Vitaly V.; Gavrilov, Sergey N. Environmental Microbiology, Vol. 15, Issue 6 https://doi.org/10.1111/1462-2920.12067	journal	January 2013
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