skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure

Journal Article · · The Plant Cell
DOI:https://doi.org/10.1105/tpc.15.00373· OSTI ID:1597748
ORCiD logo [1];  [2];  [3]; ORCiD logo [4];  [5]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [4]
  1. Purdue Univ., West Lafayette, IN (United States); Heartland Plant Innovations, Manhattan, KS (United States)
  2. Univ. of Wisconsin, Madison, WI (United States); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC); Kyoto Univ. (Japan)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  4. Purdue Univ., West Lafayette, IN (United States)
  5. Univ. of Wisconsin, Madison, WI (United States); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC)
+ Show Author Affiliations

Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding both cinnamyl alcohol dehydrogenases (CADs), including CADC and CADD, in Arabidopsis thaliana results in the atypical incorporation of hydroxycinnamaldehydes into lignin. Another strategy to change lignin composition is downregulation or overexpression of ferulate 5-hydroxylase (F5H), which results in lignins enriched in guaiacyl or syringyl units, respectively. In this work, we combined these approaches to generate plants enriched in coniferaldehyde-derived lignin units or lignins derived primarily from sinapaldehyde. The cadc cadd and ferulic acid hydroxylase1 (fah1) cadc cadd plants are similar in growth to wild-type plants even though their lignin compositions are drastically altered. In contrast, disruption of CAD in the F5H-overexpressing background results in dwarfism. The dwarfed phenotype observed in these plants does not appear to be related to collapsed xylem, a hallmark of many other lignin-deficient dwarf mutants. cadc cadd, fah1 cadc cadd, and cadd F5H-overexpressing plants have increased enzyme-catalyzed cell wall digestibility. Given that these CAD-deficient plants have similar total lignin contents and only differ in the amounts of hydroxycinnamaldehyde monomer incorporation, these results suggest that hydroxycinnamaldehyde content is a more important determinant of digestibility than lignin content.

Research Organization:
Purdue Univ., West Lafayette, IN (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; Ministry of Education, Culture, Sports, Science and Technology (MEXT)
Grant/Contract Number:
FG02-07ER15905; SC0000997; FC02-07ER64494; 26892014; FG02-06ER64301
OSTI ID:
1597748
Journal Information:
The Plant Cell, Vol. 27, Issue 8; ISSN 1040-4651
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 107 works
Citation information provided by
Web of Science

Cited By (31)

Wege zur Verwertung von Lignin: Fortschritte in der Biotechnik, der Bioraffination und der Katalyse journal June 2016
Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis journal June 2016
Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice journal April 2017
Lignin modification in planta for valorization journal January 2018
The lignin toolbox of the model grass Setaria viridis journal June 2019
Complementary iTRAQ-based proteomic and RNA sequencing-based transcriptomic analyses reveal a complex network regulating pomegranate (Punica granatum L.) fruit peel colour journal August 2018
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice journal November 2019
Manipulation of lignin metabolism by plant densities and its relationship with lodging resistance in wheat journal February 2017
Unlocking the potential of lignocellulosic biomass through plant science journal October 2015
Host lignin composition affects haustorium induction in the parasitic plants Phtheirospermum japonicum and Striga hermonthica journal March 2018
Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus journal January 2020
Simultaneous regulation of F5H in COMT ‐ RNA i transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis journal October 2018
Lignin characterization of rice CONIFERALDEHYDE 5-HYDROXYLASE loss-of-function mutants generated with the CRISPR/Cas9 system journal December 2018
Os MYB 108 loss‐of‐function enriches p ‐coumaroylated and tricin lignin units in rice cell walls journal April 2019
Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition journal February 2019
Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis journal October 2019
Comparative transcriptome analysis indicates that a core transcriptional network mediates isonuclear alloplasmic male sterility in wheat (Triticum aestivum L.) journal January 2020
The impact of alterations in lignin deposition on cellulose organization of the plant cell wall journal June 2016
RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production journal December 2016
Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana journal September 2018
Hydrolysis of untreated lignocellulosic feedstock is independent of S-lignin composition in newly classified anaerobic fungal isolate, Piromyces sp. UH3-1 journal October 2018
Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana journal May 2019
Plant-based biofuels journal January 2016
Comprehensive genome-wide analysis of the pear (Pyrus bretschneideri) laccase gene (PbLAC) family and functional identification of PbLAC1 involved in lignin biosynthesis journal February 2019
In Silico Genome-Wide Analysis of the Pear (Pyrus bretschneideri) KNOX Family and the Functional Characterization of PbKNOX1, an Arabidopsis BREVIPEDICELLUS Orthologue Gene, Involved in Cell Wall and Lignin Biosynthesis journal July 2019
Grass Cell Walls: A Story of Cross-Linking journal January 2017
Mutant Transcriptome Sequencing Provides Insights into Pod Development in Peanut (Arachis hypogaea L.) journal November 2017
Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear journal August 2018
Expression of brown-midrib in a spontaneous sorghum mutant is linked to a 5′-UTR deletion in lignin biosynthesis gene SbCAD2 journal September 2017
A Wheat Cinnamyl Alcohol Dehydrogenase TaCAD12 Contributes to Host Resistance to the Sharp Eyespot Disease journal November 2016
High-Resolution Linkage Map With Allele Dosage Allows the Identification of Regions Governing Complex Traits and Apospory in Guinea Grass (Megathyrsus maximus) journal February 2020

Similar Records

Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure
Journal Article · Sat Aug 01 00:00:00 EDT 2015 · The Plant Cell · OSTI ID:1597748
+5 more
H-lignin can be deposited independently of CINNAMYL ALCOHOL DEHYDROGENASE C and D in Arabidopsis
Journal Article · Fri May 06 00:00:00 EDT 2022 · Plant Physiology (Bethesda) · OSTI ID:1597748
+1 more
Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressing FERULATE 5-HYDROXYLASE
Journal Article · Mon Jul 24 00:00:00 EDT 2023 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1597748
+8 more

Related Subjects

09 BIOMASS FUELS