Chemical Profiling of Volatile Organic Compounds in the Headspace of Algal Cultures as Early Biomarkers of Algal Pond Crashes

Reese, Kristen L.; Fisher, Carolyn L.; Lane, Pamela D.; Jaryenneh, James D.; Moorman, Matthew W.; Jones, A. Daniel; Frank, Matthias; Lane, Todd W.

doi:10.1038/s41598-019-50125-z

Title: Chemical Profiling of Volatile Organic Compounds in the Headspace of Algal Cultures as Early Biomarkers of Algal Pond Crashes

Journal Article · Wed Sep 25 00:00:00 EDT 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-019-50125-z· OSTI ID:1570268

Reese, Kristen L. ^[1]; Fisher, Carolyn L. ^[2]; Lane, Pamela D. ^[2]; Jaryenneh, James D. ^[2]; Moorman, Matthew W. ^[3];

^[4];

^[5]; Lane, Todd W. ^[2]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Michigan State Univ., East Lansing, MI (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Michigan State Univ., East Lansing, MI (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Algae ponds used in industrial biomass production are susceptible to pathogen or grazer infestation, resulting in pond crashes with high economic costs. Current methods to monitor and mitigate unhealthy ponds are hindered by a lack of early indicators that precede culture crash. We used solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) to identify volatiles emitted from healthy and rotifer infested cultures of Microchloropsis salina. After 48 hours of algal growth, marine rotifers, Brachionus plicatilis, were added to the algae cultures and volatile organic compounds (VOC) were sampled from the headspace using SPME fibers. A GC-MS approach was used in an untargeted analysis of VOCs, followed by preliminary identification. The addition of B. plicatilis to healthy cultures of M. salina resulted in decreased algal cell numbers, relative to uninfected controls, and generated trans-β-ionone and β-cyclocitral, which were attributed to carotenoid degradation. The abundances of the carotenoid-derived VOCs increased with rotifer consumption of algae. Our results indicate that specific VOCs released by infected algae cultures may be early indicators for impending pond crashes, providing a useful tool to monitor algal biomass production and pond crash prevention.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000; AC52-07NA27344

OSTI ID:: 1570268

Alternate ID(s):: OSTI ID: 1614967

Report Number(s):: SAND-2019-10839J; LLNL-JRNL-775406; 679369

Journal Information:: Scientific Reports, Vol. 9, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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Title: Chemical Profiling of Volatile Organic Compounds in the Headspace of Algal Cultures as Early Biomarkers of Algal Pond Crashes

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