Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy
- White Dog Labs, Inc., New Castle, DE (United States)
- CelDezyner, Rehovot (Israel)
Biological CO2 fixation is an important technology that can assist in combating climate change. Here, we show an approach called anaerobic, non-photosynthetic mixotrophy can result in net CO2 fixation when using a reduced feedstock. This approach uses microbes called acetogens that are capable of concurrent utilization of both organic and inorganic substrates. In this study, we investigated the substrate utilization of 17 different acetogens, both mesophilic and thermophilic, on a variety of different carbohydrates and gases. Compared to most model acetogen strains, several non-model mesophilic strains displayed greater substrate flexibility, including the ability to utilize disaccharides, glycerol and an oligosaccharide, and growth rates. Three of these non-model strains (Blautia producta, Clostridium scatologenes and Thermoanaerobacter kivui) were chosen for further characterization, under a variety of conditions including H2- or syngas-fed sugar fermentations and a CO2-fed glycerol fermentation. In all cases, CO2 was fixed and carbon yields approached 100%. Finally, the model acetogen C. ljungdahlii was engineered to utilize glucose, a non-preferred sugar, while maintaining mixotrophic behavior. This work demonstrates the flexibility and robustness of anaerobic, non-photosynthetic mixotrophy as a technology to help reduce CO2 emissions.
- Research Organization:
- White Dog Labs, Inc., New Castle, DE (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- Grant/Contract Number:
- EE0007564
- OSTI ID:
- 1569044
- Journal Information:
- FEMS Microbiology Letters (Online), Vol. 365, Issue 8; ISSN 1574-6968
- Publisher:
- Federation of European Microbiological SocietiesCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Carbon dioxide to bio‐fuels by mixed and pure microbial cultures isolated from activated sludge: relative evaluation of CO 2 fixation, biodiesel production, and thermodynamic analysis
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journal | August 2019 |
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