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Title: Compositions and methods for making selenocysteine containing polypeptides

Patent ·
OSTI ID:1568108
; ; ;

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

Research Organization:
Yale Univ., New Haven, CT (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-98ER20311
Assignee:
Yale University (New Haven, CT)
Patent Number(s):
10,240,158
Application Number:
15/202,194
OSTI ID:
1568108
Resource Relation:
Patent File Date: 07/05/2016
Country of Publication:
United States
Language:
English

References (7)

Positional control of selenium insertion in polypeptides for X-ray crystallography patent December 1997
Selected polynucleotide and polypeptide sequences of the methanogenic archaeon, methanococcus jannashii patent January 2003
In vivo incorporation of unnatural amino acids patent-application May 2003
Compositions and Methods for Making Selenocysteine Containing Polypeptides patent-application June 2014
Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-tRNA synthetase patent May 2010
Compositions and methods for making selenocysteine containing polypeptides patent October 2016
Site-specific incorporation of phosphoserine into proteins in Escherichia coli patent July 2015

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