Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.

Booker, Anne E.; Hoyt, David W.; Meulia, Tea; Eder, Elizabeth; Nicora, Carrie D.; Purvine, Samuel O.; Daly, Rebecca A.; Moore, Joseph D.; Wunch, Kenneth; Pfiffner, Susan M.; Lipton, Mary S.; Mouser, Paula J.; Wrighton, Kelly C.; Wilkins, Michael J.; Liu, Shuang-Jiang

doi:10.1128/AEM.00018-19

Title: Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.

Journal Article · Fri Apr 12 00:00:00 EDT 2019 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.00018-19· OSTI ID:1567051

Booker, Anne E. ^[1]; Hoyt, David W. ^[2]; Meulia, Tea ^[1]; Eder, Elizabeth ^[2]; Nicora, Carrie D. ^[2]; Purvine, Samuel O. ^[2]; Daly, Rebecca A. ^[1]; Moore, Joseph D. ^[3]; Wunch, Kenneth ^[3]; Pfiffner, Susan M. ^[4]; Lipton, Mary S. ^[2];

^[5]; Wrighton, Kelly C. ^[6]; Wilkins, Michael J. ^[6]; Liu, Shuang-Jiang ^[7]

The Ohio State Univ., Columbus, OH (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
DowDuPont Industrial Biosciences, Wilmington, DE (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of New Hampshire, Durham, NH (United States)
Colorado State Univ., Fort Collins, CO (United States)
Chinese Academy of Sciences (CAS), Beijing (China)

Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolerate large changes in pressure, temperature, and salinity. Here, we used a Halanaerobium strain isolated from a natural gas well in the Utica Point Pleasant formation to investigate metabolic and physiological responses to growth under high-pressure subsurface conditions. Laboratory incubations confirmed the ability of Halanaerobium congolense strain WG8 to grow under pressures representative of deep shale formations (21 to 48 MPa). Under these conditions, broad metabolic and physiological shifts were identified, including higher abundances of proteins associated with the production of extracellular polymeric substances. Confocal laser scanning microscopy indicated that extracellular polymeric substance (EPS) production was associated with greater cell aggregation when biomass was cultured at high pressure.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1567051

Report Number(s):: PNNL-SA-144542

Journal Information:: Applied and Environmental Microbiology, Vol. 85, Issue 12; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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59 BASIC BIOLOGICAL SCIENCES
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