Genomes to ecosystem function: Targeting critical knowledge gaps in methanogenesis and translation to updated global biogeochemical models (Wrighton Ohio State Report -- Early Career Department of Energy)
- The Ohio State Univ., Columbus, OH (United States); Colorado State Univ., Fort Collins, CO (United States)
In 2018 our key deliverable was a temporally and spatially resolved investigation of the microbial community across wetland soils. As part of this experimental design we sampled 3 land coverage types at a total of 10 sites: Mud (Mud-1, Mud-2, Mud-3), Plant (Nelumbo-1, Nelumbo-2, Nelumbo-3, Typha-1), Open water (Open Water-1, Open Water-2, Open Water-3). Within each site we collected 3-5 soil cores, sub-sampled at the surface (0-5 cm), middle (10- 15 cm), and deep (25-30 cm) depths. Additionally in August, during peak methane emission, we conducted a higher-resolved depth sampling at 5 cm intervals: 0-5, 5-10, 10-15, 15-20, 20-25 and 25-30 cm depths. In total, from a single field season, we collected over 950 samples for paired microbial, geochemical, and metabolite data. Additionally, all measurements were paired to in situ soil greenhouse gas measurements, and emission chamber measurements.
- Research Organization:
- The Ohio State Univ., Columbus, OH (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Contributing Organization:
- Joint Genome Institute; Environmental Molecular Sciences Laboratory
- DOE Contract Number:
- SC0018022
- OSTI ID:
- 1562931
- Report Number(s):
- DOE-OSU-SC0018022
- Country of Publication:
- United States
- Language:
- English
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