Nucleotide Dependence of Subunit Rearrangements in Short-Form Rubisco Activase from Spinach

Peterson-Forbrook, Dayna S.; Hilton, Matthew T.; Tichacek, Laura; Henderson, J. Nathan; Bui, Hoang Q.; Wachter, Rebekka M.

doi:10.1021/acs.biochem.7b00574

Title: Nucleotide Dependence of Subunit Rearrangements in Short-Form Rubisco Activase from Spinach

Journal Article · Thu Aug 10 00:00:00 EDT 2017 · Biochemistry

DOI:https://doi.org/10.1021/acs.biochem.7b00574· OSTI ID:1534425

Peterson-Forbrook, Dayna S. ^[1]; Hilton, Matthew T. ^[1]; Tichacek, Laura ^[1]; Henderson, J. Nathan ^[1]; Bui, Hoang Q. ^[1];

^[1]

Arizona State Univ., Mesa, AZ (United States)

Higher-plant Rubisco activase (Rca) plays a critical role in regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In vitro, Rca is known to undergo dynamic assembly–disassembly processes, with several oligomer stoichiometries coexisting over a broad concentration range. Although the hexamer appears to be the active form, changes in quaternary structure could play a role in Rubisco regulation. Therefore, in this work, fluorescent labels were attached to the C-termini of spinach β-Rca, and the rate of subunit mixing was monitored by measuring energy transfer as a function of nucleotide and divalent cation. Only dimeric units appeared to exchange. Poorly hydrolyzable substrate analogues provided locked complexes with high thermal stabilities (apparent T_m = 60 °C) and an estimated t_1/2 of at least 7 h, whereas ATP-Mg provided tight assemblies with t_1/2 values of 30–40 min and ADP-Mg loose assemblies with t_1/2 values of <15 min. Accumulation of ADP to 20% of the total level of adenine nucleotide substantially accelerated equilibration. An initial lag period was observed with ATP·Mg, indicating inhibition of subunit exchange at low ADP concentrations. The ADP K_i value was estimated to exceed the K_m for ATP (0.772 ± 96 mM), suggesting that the equilibration rate is a function of the relative contributions of high- and low-affinity states. C-Terminal cross-linking generated covalent dimers, required the N-terminal extension to the AAA+ domain, and provided evidence of different classes of sites. We propose that oligomer reorganization may be stalled during periods of high Rubisco reactivation activity, whereas changes in quaternary structure are stimulated by the accumulation of ADP at low light levels.

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Research Organization:: Arizona State Univ., Tempe, AZ (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0002423; FG02-09-ER16123

OSTI ID:: 1534425

Journal Information:: Biochemistry, Vol. 56, Issue 36; ISSN 0006-2960

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 4 works

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Single-molecule diffusometry reveals the nucleotide-dependent oligomerization pathways of Nicotiana tabacum Rubisco activase Wang, Quan; Serban, Andrew J.; Wachter, Rebekka M. The Journal of Chemical Physics, Vol. 148, Issue 12 https://doi.org/10.1063/1.5005930	journal	March 2018
Cyanobacterial carboxysomes contain an unique rubisco‐activase‐like protein Lechno‐Yossef, Sigal; Rohnke, Brandon A.; Belza, Ana C. O. New Phytologist, Vol. 225, Issue 2 https://doi.org/10.1111/nph.16195	journal	October 2019
Probing the rice Rubisco–Rubisco activase interaction via subunit heterooligomerization Shivhare, Devendra; Ng, Jediael; Tsai, Yi-Chin Candace Proceedings of the National Academy of Sciences, Vol. 116, Issue 48 https://doi.org/10.1073/pnas.1914245116	journal	November 2019

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