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Title: Method for labeling DNA and RNA

Patent ·
OSTI ID:1531528
;

A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.

Research Organization:
U Chicago Argonne LLC, Chicago, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-31-109-ENG-38
Assignee:
U Chicago Argonne LLC (Chicago, IL)
Patent Number(s):
7,208,269
Application Number:
10/057,753
OSTI ID:
1531528
Resource Relation:
Patent File Date: 2002-01-23
Country of Publication:
United States
Language:
English

References (5)

Process for labeling nucleic acids and hybridization probes patent October 1986
Methods for immobilizing nucleic acids on a gel substrate patent November 1999
Column device for isolation and labeling of nucleic acids patent-application May 2003
Automated sequencing of fluorescently labelled DNA by chemical degradation journal January 1990
Column device for isolation and labeling of nucleic acids patent November 2004

Cited By (6)

Nucleic acid purification with a binding matrix patent February 2014
Methods of purifying a nucleic acid and formulation and kit for use in performing such methods patent October 2011
Nucleic acid purification with a binding matrix patent October 2011
Methods of optimal purification of nucleic acids and kit for use in performing such methods patent July 2012
Universal nucleic acids sample preparation method for cells, spores and their mixture patent January 2011
Methods of purifying a nucleic acid and formulation and kit for use in performing such methods patent August 2013

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59 BASIC BIOLOGICAL SCIENCES
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