Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes

Gilbert, Sarah; Xu, Jenny; Acosta, Kenneth; Poulev, Alexander; Lebeis, Sarah; Lam, Eric

doi:10.3389/fchem.2018.00265

Title: Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes

Journal Article · Thu Jul 12 00:00:00 EDT 2018 · Frontiers in Chemistry

DOI:https://doi.org/10.3389/fchem.2018.00265· OSTI ID:1510429

Gilbert, Sarah ^[1]; Xu, Jenny ^[1]; Acosta, Kenneth ^[1]; Poulev, Alexander ^[1]; Lebeis, Sarah ^[2]; Lam, Eric ^[1]

Rutgers Univ., New Brunswick, NJ (United States). Dept. of Plant Biology
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology

Duckweed farming can be a sustainable practice for biofuel production, animal feed supplement, and wastewater treatment, although large scale production remains a challenge. Plant growth promoting bacteria (PGPB) have been shown to improve plant health by producing phytohormones such as auxin. While some of the mechanisms for plant growth promotion have been characterized in soil epiphytes, more work is necessary to understand how plants may select for bacterial endophytes that have the ability to provide an exogenous source of phytohormones such as auxin. We have isolated and characterized forty-seven potentially endophytic bacteria from surface-sterilized duckweed tissues and screened these bacterial strains for production of indole related compounds using the Salkowski colorimetric assay. Indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indole produced by various bacterial isolates were verified by mass spectrometry. Using the Salkowski reagent, we found that 79% of the isolated bacterial strains from our collection may be capable of producing indole related compounds to various extents during in vitro growth. Of these bacteria that are producing indole related compounds, 19% are additionally producing indole. There is an apparent correlation between the type of indole related compound produced by a particular bacteria and the duckweed genus from which the bacterial strain is derived. These results suggest the possible association between different duckweed genera and endophytes that are producing distinct types of secondary metabolites. Understanding the role of indole related compounds during interaction between endophytes and the plant host may be useful to help design synthetic bacterial communities that could target specific or multiple species of duckweed in the future to sustainably enhance plant growth.

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Research Organization:: Rutgers Univ., New Brunswick, NJ (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018244

OSTI ID:: 1510429

Journal Information:: Frontiers in Chemistry, Vol. 6; ISSN 2296-2646

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 45 works

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Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants Sakurai, Takuma; Odamaki, Toshitaka; Xiao, Jin-zhong Microorganisms, Vol. 7, Issue 9 https://doi.org/10.3390/microorganisms7090340	journal	September 2019

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59 BASIC BIOLOGICAL SCIENCES
auxin
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duckweeds
indoles
Salkowski assay
endophytes

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