Strain Distributions and Structural Changes in Motor Driven Gels (Final Report)
Abstract
The goal of this project was to study the effects of DNA-based, force-generating motor proteins on the structure and dynamics of a DNA hydrogel. Motor proteins are nanoscale transducers, converting chemical energy embedded in the solution into local mechanical work on hydrogel strands, and thus potentially driving structural changes and/or non-equilibrium dynamics within the gel material. To explore this, we used self-assembly to create condensed DNA phases, and activated the phases with proteins. The specific aim was to study the deformations (strain fields) generated within a DNA gel by motor forces. We succeeded in this aim, developing methods to experimentally create gel/motor systems, and measure strain with high spatio-temporal resolution. A key finding was that simple continuum strain-field models fail to describe the data. A second major outcome was the development of novel models of hydrogel elasticity incorporating solvent effects that can be used to model dynamic motor-driven strains. A third major outcome was to learn how to control the phase and structure of condensed DNA particles, including both liquid-crystalline behavior, and the formation of DNA liquids.
- Authors:
-
- Univ. of California, Santa Barbara, CA (United States)
- Publication Date:
- Research Org.:
- Univ. of California, Santa Barbara, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1509714
- Report Number(s):
- DOE-UCSB-0014427
- DOE Contract Number:
- SC0014427
- Resource Type:
- Technical Report
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Saleh, Omar, Fygenson, Deborah, and McMeeking, Robert. Strain Distributions and Structural Changes in Motor Driven Gels (Final Report). United States: N. p., 2019.
Web. doi:10.2172/1509714.
Saleh, Omar, Fygenson, Deborah, & McMeeking, Robert. Strain Distributions and Structural Changes in Motor Driven Gels (Final Report). United States. https://doi.org/10.2172/1509714
Saleh, Omar, Fygenson, Deborah, and McMeeking, Robert. 2019.
"Strain Distributions and Structural Changes in Motor Driven Gels (Final Report)". United States. https://doi.org/10.2172/1509714. https://www.osti.gov/servlets/purl/1509714.
@article{osti_1509714,
title = {Strain Distributions and Structural Changes in Motor Driven Gels (Final Report)},
author = {Saleh, Omar and Fygenson, Deborah and McMeeking, Robert},
abstractNote = {The goal of this project was to study the effects of DNA-based, force-generating motor proteins on the structure and dynamics of a DNA hydrogel. Motor proteins are nanoscale transducers, converting chemical energy embedded in the solution into local mechanical work on hydrogel strands, and thus potentially driving structural changes and/or non-equilibrium dynamics within the gel material. To explore this, we used self-assembly to create condensed DNA phases, and activated the phases with proteins. The specific aim was to study the deformations (strain fields) generated within a DNA gel by motor forces. We succeeded in this aim, developing methods to experimentally create gel/motor systems, and measure strain with high spatio-temporal resolution. A key finding was that simple continuum strain-field models fail to describe the data. A second major outcome was the development of novel models of hydrogel elasticity incorporating solvent effects that can be used to model dynamic motor-driven strains. A third major outcome was to learn how to control the phase and structure of condensed DNA particles, including both liquid-crystalline behavior, and the formation of DNA liquids.},
doi = {10.2172/1509714},
url = {https://www.osti.gov/biblio/1509714},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Apr 29 00:00:00 EDT 2019},
month = {Mon Apr 29 00:00:00 EDT 2019}
}
Works referenced in this record:
Poroelastic toughening in polymer gels: A theoretical and numerical study
journal, September 2016
- Noselli, Giovanni; Lucantonio, Alessandro; McMeeking, Robert M.
- Journal of the Mechanics and Physics of Solids, Vol. 94
Tuning phase and aging of DNA hydrogels through molecular design
journal, January 2017
- Nguyen, Dan T.; Saleh, Omar A.
- Soft Matter, Vol. 13, Issue 32
A Model for the Mullins Effect in Multinetwork Elastomers
journal, October 2017
- Bacca, Mattia; Creton, Costantino; McMeeking, Robert M.
- Journal of Applied Mechanics, Vol. 84, Issue 12
Electrostatics and depletion determine competition between 2D nematic and 3D bundled phases of rod-like DNA nanotubes
journal, January 2016
- Park, Chang-Young; Fygenson, Deborah K.; Saleh, Omar A.
- Soft Matter, Vol. 12, Issue 23
Engineering the Mechanical Behavior of Polymer Networks with Flexible Self-Assembled V-Shaped Monomers
journal, April 2018
- Cohen, Noy; Saleh, Omar A.; McMeeking, Robert M.
- Macromolecules, Vol. 51, Issue 8
A viscoelastic constitutive law for hydrogels
journal, February 2017
- Bacca, Mattia; McMeeking, Robert M.
- Meccanica, Vol. 52, Issue 14
Salt-dependent properties of a coacervate-like, self-assembled DNA liquid
journal, January 2018
- Jeon, Byoung-jin; Nguyen, Dan T.; Abraham, Gabrielle R.
- Soft Matter, Vol. 14, Issue 34