Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells

Kijima, Saku T.; Staiger, Christopher J.; Katoh, Kaoru; Nagasaki, Akira; Ito, Kohji; Uyeda, Taro Q.  P.

doi:10.1038/s41598-018-22707-w

Title: Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells

Journal Article · Mon Mar 12 00:00:00 EDT 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-22707-w· OSTI ID:1506650

Kijima, Saku T. ^[1]; Staiger, Christopher J. ^[2]; Katoh, Kaoru ^[1]; Nagasaki, Akira ^[3]; Ito, Kohji ^[4]; Uyeda, Taro Q. P. ^[5]

National Institute of Advanced Industrial Science and Technology, Ibaraki (Japan); University of Tsukuba, Ibaraki (Japan)
Purdue Univ., West Lafayette, IN (United States)
National Institute of Advanced Industrial Science and Technology, Ibaraki (Japan)
Chiba University (Japan)
National Institute of Advanced Industrial Science and Technology, Ibaraki (Japan); Waseda Univ., Tokyo (Japan)

Flowering plants express multiple actin isoforms. Previous studies suggest that individual actin isoforms have specific functions; however, the subcellular localization of actin isoforms in plant cells remains obscure. Here, we transiently expressed and observed major Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, as fluorescent-fusion proteins. By optimizing the linker sequence between fluorescent protein and actin, we succeeded in observing filaments that contained these expressed actin isoforms fused with green fluorescent protein (GFP) in Arabidopsis protoplasts. Different colored fluorescent proteins fused with AtACT2 and AtACT7 and co-expressed in Nicotiana benthamiana mesophyll cells co-polymerized in a segregated manner along filaments. In epidermal cells, surprisingly, AtACT2 and AtACT7 tended to polymerize into different types of filaments. AtACT2 was incorporated into thinner filaments, whereas AtACT7 was incorporated into thick bundles. We conclude that different actin isoforms are capable of constructing unique filament arrays, depending on the cell type or tissue. Interestingly, staining patterns induced by two indirect actin filament probes, Lifeact and mTalin1, were different between filaments containing AtACT2 and those containing AtACT7. We suggest that filaments containing different actin isoforms bind specific actin-binding proteins in vivo, since the two probes comprise actin-binding domains from different actin-binding proteins.

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Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-04ER15526

OSTI ID:: 1506650

Journal Information:: Scientific Reports, Vol. 8, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 15 works

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