Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production

Williams, Daniel L.; Ong, Rebecca G.; Mullet, John E.; Hodge, David B.

doi:10.3389/fenrg.2018.00133

Title: Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production

Journal Article · Mon Jan 14 00:00:00 EST 2019 · Frontiers in Energy Research

DOI:https://doi.org/10.3389/fenrg.2018.00133· OSTI ID:1506483

Williams, Daniel L. ^[1]; Ong, Rebecca G. ^[2]; Mullet, John E. ^[3]; Hodge, David B. ^[4]

Michigan State Univ., East Lansing, MI (United States); Michigan State University, Madison, MI (United States)
Michigan State University, Madison, MI (United States); Michigan Technological University, Houghton, MI (United States)
Michigan State University, Madison, MI (United States); Texas A & M Univ., College Station, TX (United States)
Montana State Univ., Bozeman, MT (United States); Luleå University of Technology (Sweden)

Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed by mild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., <90°C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (>8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolved to ferment xylose.

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Research Organization:: Great Lakes Bioenergy Research Center, Madison, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409; FC02-07ER64494

OSTI ID:: 1506483

Journal Information:: Frontiers in Energy Research, Vol. 6; ISSN 2296-598X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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