Assembly of nucleic acid sequences in emulsions

Title: Assembly of nucleic acid sequences in emulsions

Patent · Tue Feb 12 00:00:00 EST 2019

OSTI ID:1502836

Church, George M.; Terry, Richard C.; Kosuri, Sriram; Zhang, Di

Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided.

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High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides Borovkov, Alex Y.; Loskutov, Andrey V.; Robida, Mark D. Nucleic Acids Research, Vol. 38, Issue 19 https://doi.org/10.1093/nar/gkq677	journal	August 2010
Methods and devices for high fidelity polynucleotide synthesis Jacobson, Joseph M.; Church, George M.; Chu, Larry Li-Yang https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8808986 US Patent Document 8,808,986	patent	August 2014
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips Kosuri, Sriram; Eroshenko, Nikolai; LeProust, Emily M. Nature Biotechnology, Vol. 28, Issue 12, p. 1295-1299 https://doi.org/10.1038/nbt.1716	journal	November 2010
Next-generation DNA sequencing Shendure, Jay; Ji, Hanlee Nature Biotechnology, Vol. 26, Issue 10 https://doi.org/10.1038/nbt1486	journal	October 2008

The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY