Development of a Chemoenzymatic-like and Photoswitchable Method for the Ordered Attachment of Proteins to Surfaces
Protein arrays are the best tool for the rapid analysis of a whole proteome thus helping to identify all the protein/protein interactions in a living cell and they can also be used as powerful biosensors. The objective of this proposal is to develop a new entropically activated ligation method based in the naturally occurring protein trans-splicing process. This method will be used for the generation of spatially addressable arrays of multiple protein components by standard photolithographic techniques. Key to our approach is the use of the protein trans-splicing process. This naturally occurring process will allow us to create a truly generic and highly efficient method for the covalent attachment of proteins through its C-terminus to any solid support.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 15014684
- Report Number(s):
- UCRL-TR-209731; TRN: US200807%%701
- Resource Relation:
- Other Information: PBD: 11 Feb 2005
- Country of Publication:
- United States
- Language:
- English
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Photoswitchable method for the ordered attachment of proteins to surfaces
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