skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images

Conference ·
; ; ;

The objectives of this work include automatic recovery and visualization of a 3D chromosome structure from a sequence of 2D tomographic reconstruction images taken through the nucleus of a cell. Structure is very important for biologists as it affects chromosome functions, behavior of the cell and its state. Chromosome analysis is significant in the detection of deceases and in monitoring environmental gene mutations. The algorithm incorporates thresholding based on a histogram analysis with a polyline splitting algorithm, contour extraction via active contours, and detection of the 3D chromosome structure by establishing corresponding regions throughout the slices. Visualization using point cloud meshing generates a 3D surface. The 3D triangular mesh of the chromosomes provides surface detail and allows a user to interactively analyze chromosomes using visualization software.

Research Organization:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
OSTI ID:
15014143
Report Number(s):
UCRL-PROC-203893; TRN: US200805%%308
Resource Relation:
Journal Volume: 2006; Journal Issue: 1; Conference: Presented at: IEEE Workshop on Articulated and Nonrigid, Washington, DC, United States, Jun 27 - Jun 27, 2004
Country of Publication:
United States
Language:
English

References (17)

Automated segmentation of necrotic femoral head from 3D MR data journal July 2004
Accurate 3D reconstruction of complex blood vessel geometries from intravascular ultrasound images: in vitro study journal January 2000
Semi-automated color segmentation of anatomical tissue journal May 2000
Individual Comparisons by Ranking Methods journal December 1945
An experimental comparison of range image segmentation algorithms journal July 1996
Deformable models in medical image analysis: a survey journal June 1996
Registration, segmentation, and visualization of multimodal brain images journal March 2001
Lymph node segmentation from CT images using fast marching method journal January 2004
A classification-driven partially occluded object segmentation (CPOOS) method with application to chromosome analysis journal January 1998
Detection and Characterization of Isolated and Overlapping Spots journal April 1998
A system for brain tumor volume estimation via MR imaging and fuzzy connectedness journal January 2005
Segmentation and visualization of brain lesions in multispectral magnetic resonance images journal March 1995
Using the Hilbert transform for 3D visualization of differential interference contrast microscope images journal July 2000
A Fast algorithm for active contours and curvature estimation journal January 1992
Techniques for efficient, real-time, 3D visualization of multi-modality cardiac data using consumer graphics hardware journal September 2005
A fast snake model based on non-linear diffusion for medical image segmentation journal April 2004
Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei journal January 1989

Similar Records

Related Subjects

42 ENGINEERING
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
59 BASIC BIOLOGICAL SCIENCES
ALGORITHMS
CHROMOSOMES
DETECTION
GENE MUTATIONS
MONITORING