Assembly of Oriented Virus Arrays by Chemo-Selective Ligation Methods and Nanolithography Techniques
The present work describes our ongoing efforts towards the creation of nano-scaled ordered arrays of protein/virus covalently attached to site-specific chemical linkers patterned by different nanolithograpy techniques. We will present a new and efficient solid-phase approach for the synthesis of chemically modified long alkyl-thiols. These compounds can be used to introduce chemoselective reacting groups onto gold and silicon-based surfaces. Furthermore, these modified thiols have been used to create nanometric patterns by using different nanolithography techniques. We will show that these patterns can react chemoselectively with proteins and/or virus which have been chemically or recombinantly modified to contain complementary chemical groups at specific positions thus resulting in the oriented attachment of the protein or virus to the surface.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 15003341
- Report Number(s):
- UCRL-JC-151507; TRN: US200431%%43
- Resource Relation:
- Conference: 2003 Nanotechnology Conference and Trade Show, San Francisco, CA (US), 02/23/2003--02/27/2003; Other Information: PBD: 2 Dec 2002
- Country of Publication:
- United States
- Language:
- English
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