The effect of alkali-soluble lignin on purified core cellulase and hemicellulase activities during hydrolysis of extractive ammonia-pretreated lignocellulosic biomass
- Guangxi Univ., Nanning (China). State Key Lab. for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center (GLBRC), Biomass Conversion Research Laboratory (BCRL), Dept. of Chemical Engineering and Materials Science
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center (GLBRC), Biomass Conversion Research Laboratory (BCRL), Dept. of Chemical Engineering and Materials Science
- Guangxi Univ., Nanning (China). State Key Lab. for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology
- Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center (GLBRC), Biomass Conversion Research Laboratory (BCRL), Dept. of Chemical Engineering and Materials Science; Univ. of Houston, Houston, TX (United State). Dept. of Engineering Technology, Biotechnology Division, School of Technology
Removing alkali-soluble lignin using extractive ammonia (EA) pretreatment of corn stover (CS) is known to improve biomass conversion efficiency during enzymatic hydrolysis. In this study, we investigated the effect of alkali-soluble lignin on six purified core glycosyl hydrolases and their enzyme synergies, adopting 31 enzyme combinations derived by a five-component simplex centroid model, during EA-CS hydrolysis. Hydrolysis experiment was carried out using EA-CS(-) (approx. 40% lignin removed during EA pretreatment) and EA-CS(+) (where no lignin was extracted). Enzymatic hydrolysis experiments were done at three different enzyme mass loadings (7.5, 15 and 30 mg protein g-1 glucan), using a previously developed high-throughput microplate-based protocol, and the sugar yields of glucose and xylose were detected. The optimal enzyme combinations (based on % protein mass loading) of six core glycosyl hydrolases for EA-CS(-) and EA-CS(+) were determined that gave high sugar conversion. The inhibition of lignin on optimal enzyme ratios was studied, by adding fixed amount of alkali-soluble lignin fractions to EA-CS(-), and pure Avicel, beechwood xylan and evaluating their sugar conversion. The optimal enzyme ratios that gave higher sugar conversion for EA-CS(-) were CBH I: 27.2–28.2%, CBH II: 18.2–22.2%, EG I: 29.2–34.3%, EX: 9.0–14.1%, βX: 7.2–10.2%, βG: 1.0–5.0% (at 7.5–30 mg g-1 protein mass loading). Endoglucanase was inhibited to a greater extent than other core cellulases and xylanases by lignin during enzyme hydrolysis. We also found that alkali-soluble lignin inhibits cellulase more strongly than hemicellulase during the course of enzyme hydrolysis.
- Research Organization:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- FC02-07ER64494
- OSTI ID:
- 1499908
- Journal Information:
- Royal Society Open Science, Vol. 5, Issue 6; ISSN 2054-5703
- Publisher:
- The Royal Society PublishingCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Aqueous Ammonia Pre-treatment of Wheat Straw: Process Optimization and Broad Spectrum Dye Adsorption on Nitrogen-Containing Lignin
|
journal | August 2019 |
Similar Records
Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass
Enzymatic Hydrolysis of Cellulosic Biomass