Genome analysis of the ubiquitous boxwood pathogen Pseudonectria foliicola

Rivera, Yazmín; Salgado-Salazar, Catalina; Veltri, Daniel; Malapi-Wight, Martha; Crouch, Jo Anne

doi:10.7717/peerj.5401

Title: Genome analysis of the ubiquitous boxwood pathogen Pseudonectria foliicola

Journal Article · Fri Aug 24 00:00:00 EDT 2018 · PeerJ

DOI:https://doi.org/10.7717/peerj.5401· OSTI ID:1494690

Rivera, Yazmín ^[1]; Salgado-Salazar, Catalina ^[2]; Veltri, Daniel ^[3]; Malapi-Wight, Martha ^[4]; Crouch, Jo Anne ^[5]

Mycology and Nematology Genetic Diversity and Biology Laboratory, US Department of Agriculture, Agriculture Research Service (USDA-ARS), Beltsville, MD (United States); The State University of New Jersey, New Brunswick, NJ (United States); Center for Plant Health, Science and Technology, USDA, Animal and Plant Health Inspection Service, Beltsville, MD (United States)
Mycology and Nematology Genetic Diversity and Biology Laboratory, US Department of Agriculture, Agriculture Research Service (USDA-ARS), Beltsville, MD (United States); The State University of New Jersey, New Brunswick, NJ (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Mycology and Nematology Genetic Diversity and Biology Laboratory, US Department of Agriculture, Agriculture Research Service (USDA-ARS), Beltsville, MD (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States); National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD (United States)
Mycology and Nematology Genetic Diversity and Biology Laboratory, US Department of Agriculture, Agriculture Research Service (USDA-ARS), Beltsville, MD (United States); Plant Germplasm Quarantine Program, USDA, Animal and Plant Health Inspection Service, Beltsville, MD (United States)
Mycology and Nematology Genetic Diversity and Biology Laboratory, US Department of Agriculture, Agriculture Research Service (USDA-ARS), Beltsville, MD (United States)

Boxwood (Buxusspp.) are broad-leaved, evergreen landscape plants valued for their longevity and ornamental qualities. Volutella leaf and stem blight, caused by the ascomycete fungi Pseudonectria foliicola and P. buxi, is one of the major diseases affecting the health and ornamental qualities of boxwood. Although this disease is less severe than boxwood blight caused by Calonectria pseudonaviculata and C. henricotiae, its widespread occurrence and disfiguring symptoms have caused substantial economic losses to the ornamental industry. In this study, we sequenced the genome of P. foliicola isolate ATCC13545 using Illumina technology and compared it to other publicly available fungal pathogen genomes to better understand the biology of this organism. A de novo assembly estimated the genome size of P. foliicola at 28.7 Mb (425 contigs; N50 = 184,987 bp; avg. coverage 188×), with just 9,272 protein-coding genes. To our knowledge,P. foliicola has the smallest known genome within the Nectriaceae. Consistent with the small size of the genome, the secretome, CAzyme and secondary metabolite profiles of this fungus are reduced relative to two other surveyed Nectriaceae fungal genomes:Dactylonectria macrodidyma JAC15-245 and Fusarium graminearum Ph-1. Interestingly, a large cohort of genes associated with reduced virulence and loss of pathogenicity was identified from the P. foliicoladataset. These data are consistent with the latest observations by plant pathologists that P. buxi and most likely P. foliicola, are opportunistic, latent pathogens that prey upon weak and stressed boxwood plants.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-06OR23100

OSTI ID:: 1494690

Journal Information:: PeerJ, Vol. 6; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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References (82)

The Pathogen-Host Interactions database (PHI-base): additions and future developments Urban, Martin; Pant, Rashmi; Raghunath, Arathi Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1165	journal	November 2014
QUAST: quality assessment tool for genome assemblies Gurevich, Alexey; Saveliev, Vladislav; Vyahhi, Nikolay Bioinformatics, Vol. 29, Issue 8 https://doi.org/10.1093/bioinformatics/btt086	journal	February 2013
raxmlGUI: a graphical front-end for RAxML Silvestro, Daniele; Michalak, Ingo Organisms Diversity & Evolution, Vol. 12, Issue 4 https://doi.org/10.1007/s13127-011-0056-0	journal	September 2011
A Phylogenetic Estimation of Trophic Transition Networks for Ascomycetous Fungi: Are Lichens Cradles of Symbiotrophic Fungal Diversification? Arnold, A. Elizabeth; Miadlikowska, Jolanta; Higgins, K. Lindsay Systematic Biology, Vol. 58, Issue 3 https://doi.org/10.1093/sysbio/syp001	journal	June 2009
Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection Ye, Xiaozhen; Liu, Hongyi; Jin, Yajie PLOS ONE, Vol. 12, Issue 1 https://doi.org/10.1371/journal.pone.0169598	journal	January 2017
Draft Genome Sequence of Dactylonectria macrodidyma , a Plant-Pathogenic Fungus in the Nectriaceae Malapi-Wight, Martha; Salgado-Salazar, Catalina; Demers, Jill Genome Announcements, Vol. 3, Issue 2 https://doi.org/10.1128/genomeA.00278-15	journal	April 2015
A Methylated Neurospora 5S rRNA Pseudogene Contains a Transposable Element Inactivated by Repeat-Induced Point Mutation Margolin, Brian S.; Garrett-Engele, Phillip W.; Stevens, Judith N. Genetics, Vol. 149, Issue 4 https://doi.org/10.1093/genetics/149.4.1787	journal	August 1998
Genomic and Secretomic Analyses Reveal Unique Features of the Lignocellulolytic Enzyme System of Penicillium decumbens Liu, Guodong; Zhang, Lei; Wei, Xiaomin PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0055185	journal	February 2013
Draft Genome Sequence of Neurospora crassa Strain FGSC 73 Baker, Scott E.; Schackwitz, Wendy; Lipzen, Anna Genome Announcements, Vol. 3, Issue 2 https://doi.org/10.1128/genomea.00074-15	journal	March 2015
Comparative analysis of Fusarium mitochondrial genomes reveals a highly variable region that encodes an exceptionally large open reading frame Al-Reedy, Rasha M.; Malireddy, Rahul; Dillman, Casey B. Fungal Genetics and Biology, Vol. 49, Issue 1 https://doi.org/10.1016/j.fgb.2011.11.008	journal	January 2012
Estimating divergence times in large molecular phylogenies Tamura, K.; Battistuzzi, F. U.; Billing-Ross, P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 47 https://doi.org/10.1073/pnas.1213199109	journal	November 2012
Diverse Lifestyles and Strategies of Plant Pathogenesis Encoded in the Genomes of Eighteen Dothideomycetes Fungi Ohm, Robin A.; Feau, Nicolas; Henrissat, Bernard PLoS Pathogens, Vol. 8, Issue 12 https://doi.org/10.1371/journal.ppat.1003037	journal	December 2012
Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae Galagan, James E.; Calvo, Sarah E.; Cuomo, Christina Nature, Vol. 438, Issue 7071 https://doi.org/10.1038/nature04341	journal	December 2005
The oldest fossil evidence of animal parasitism by fungi supports a Cretaceous diversification of fungal–arthropod symbioses Sung, Gi-Ho; Poinar, George O.; Spatafora, Joseph W. Molecular Phylogenetics and Evolution, Vol. 49, Issue 2 https://doi.org/10.1016/j.ympev.2008.08.028	journal	November 2008
MITOS: Improved de novo metazoan mitochondrial genome annotation Bernt, Matthias; Donath, Alexander; Jühling, Frank Molecular Phylogenetics and Evolution, Vol. 69, Issue 2 https://doi.org/10.1016/j.ympev.2012.08.023	journal	November 2013
Comparative Genomics of a Plant-Pathogenic Fungus, Pyrenophora tritici-repentis , Reveals Transduplication and the Impact of Repeat Elements on Pathogenicity and Population Divergence Manning, Viola A.; Pandelova, Iovanna; Dhillon, Braham G3: Genes\|Genomes\|Genetics, Vol. 3, Issue 1 https://doi.org/10.1534/g3.112.004044	journal	January 2013
Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea Amselem, Joelle; Cuomo, Christina A.; van Kan, Jan A. L. PLoS Genetics, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pgen.1002230	journal	August 2011
PHI-base update: additions to the pathogen host interaction database Winnenburg, R.; Urban, M.; Beacham, A. Nucleic Acids Research, Vol. 36, Issue Database https://doi.org/10.1093/nar/gkm858	journal	December 2007
Considering Transposable Element Diversification in De Novo Annotation Approaches Flutre, Timothée; Duprat, Elodie; Feuillet, Catherine PLoS ONE, Vol. 6, Issue 1 https://doi.org/10.1371/journal.pone.0016526	journal	January 2011
Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi Zhao, Zhongtao; Liu, Huiquan; Wang, Chenfang BMC Genomics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2164-14-274	journal	January 2013
Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma Kubicek, Christian P.; Herrera-Estrella, Alfredo; Seidl-Seiboth, Verena Genome Biology, Vol. 12, Issue 4 https://doi.org/10.1186/gb-2011-12-4-r40	journal	April 2011
Repbase Update, a database of repetitive elements in eukaryotic genomes Bao, Weidong; Kojima, Kenji K.; Kohany, Oleksiy Mobile DNA, Vol. 6, Issue 1 https://doi.org/10.1186/s13100-015-0041-9	journal	June 2015
MUSCLE: multiple sequence alignment with high accuracy and high throughput Edgar, R. C. Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797 https://doi.org/10.1093/nar/gkh340	journal	March 2004
Improvement of Phylogenies after Removing Divergent and Ambiguously Aligned Blocks from Protein Sequence Alignments Talavera, Gerard; Castresana, Jose Systematic Biology, Vol. 56, Issue 4 https://doi.org/10.1080/10635150701472164	journal	August 2007
Fungal Infection of Plants Knogge, Wolfgang The Plant Cell, Vol. 8, Issue 10 https://doi.org/10.2307/3870224	journal	October 1996
Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina Islam, Md; Haque, Md; Islam, Mohammad BMC Genomics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2164-13-493	journal	January 2012
The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics Cantarel, B. L.; Coutinho, P. M.; Rancurel, C. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn663	journal	January 2009
Fungal Genomics Challenges the Dogma of Name-Based Biosecurity McTaggart, Alistair R.; van der Nest, Magriet A.; Steenkamp, Emma T. PLOS Pathogens, Vol. 12, Issue 5 https://doi.org/10.1371/journal.ppat.1005475	journal	May 2016
antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences Medema, Marnix H.; Blin, Kai; Cimermancic, Peter Nucleic Acids Research, Vol. 39, Issue suppl_2 https://doi.org/10.1093/nar/gkr466	journal	June 2011
Characterization and taxonomic reassessment of the box blight pathogen Calonectria pseudonaviculata , introducing Calonectria henricotiae sp. nov. Gehesquière, B.; Crouch, J. A.; Marra, R. E. Plant Pathology, Vol. 65, Issue 1 https://doi.org/10.1111/ppa.12401	journal	May 2015
Genome Expansion and Gene Loss in Powdery Mildew Fungi Reveal Tradeoffs in Extreme Parasitism Spanu, Pietro D.; Abbott, James C.; Amselem, Joelle Science, Vol. 330, Issue 6010 https://doi.org/10.1126/science.1194573	journal	December 2010
RIPCAL: a tool for alignment-based analysis of repeat-induced point mutations in fungal genomic sequences Hane, James K.; Oliver, Richard P. BMC Bioinformatics, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2105-9-478	journal	November 2008
Clustal W and Clustal X version 2.0 Larkin, M. A.; Blackshields, G.; Brown, N. P. Bioinformatics, Vol. 23, Issue 21, p. 2947-2948 https://doi.org/10.1093/bioinformatics/btm404	journal	September 2007
Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) Martinez, Diego; Berka, Randy M.; Henrissat, Bernard Nature Biotechnology, Vol. 26, Issue 5 https://doi.org/10.1038/nbt1403	journal	May 2008
Tree of Life Reveals Clock-Like Speciation and Diversification Hedges, S. Blair; Marin, Julie; Suleski, Michael Molecular Biology and Evolution, Vol. 32, Issue 4 https://doi.org/10.1093/molbev/msv037	journal	March 2015
MAKER2: an annotation pipeline and genome-database management tool for second-generation genome projects Holt, Carson; Yandell, Mark BMC Bioinformatics, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2105-12-491	journal	December 2011
Fungal Effectors and Plant Susceptibility Lo Presti, Libera; Lanver, Daniel; Schweizer, Gabriel Annual Review of Plant Biology, Vol. 66, Issue 1 https://doi.org/10.1146/annurev-arplant-043014-114623	journal	April 2015
Pseudonectria buxi causing leaf and stem blight on Buxus in Canada Shi, Fang; Hsiang, Tom European Journal of Plant Pathology, Vol. 138, Issue 4 https://doi.org/10.1007/s10658-013-0348-7	journal	December 2013
Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis Kämper, Jörg; Kahmann, Regine; Bölker, Michael Nature, Vol. 444, Issue 7115 https://doi.org/10.1038/nature05248	journal	November 2006
AUGUSTUS: a web server for gene finding in eukaryotes Stanke, M.; Steinkamp, R.; Waack, S. Nucleic Acids Research, Vol. 32, Issue Web Server https://doi.org/10.1093/nar/gkh379	journal	July 2004
Microarrays for identifying binding sites and probing structure of RNAs Kierzek, Ryszard; Turner, Douglas H.; Kierzek, Elzbieta Nucleic Acids Research, Vol. 43, Issue 1 https://doi.org/10.1093/nar/gku1303	journal	December 2014
Regulation of Secondary Metabolism in Filamentous Fungi Yu, Jae-Hyuk; Keller, Nancy Annual Review of Phytopathology, Vol. 43, Issue 1 https://doi.org/10.1146/annurev.phyto.43.040204.140214	journal	September 2005
Plant pathogens as a source of diverse enzymes for lignocellulose digestion Gibson, Donna M.; King, Brian C.; Hayes, Marshall L. Current Opinion in Microbiology, Vol. 14, Issue 3 https://doi.org/10.1016/j.mib.2011.04.002	journal	June 2011
OrthoVenn: a web server for genome wide comparison and annotation of orthologous clusters across multiple species Wang, Yi; Coleman-Derr, Devin; Chen, Guoping Nucleic Acids Research, Vol. 43, Issue W1 https://doi.org/10.1093/nar/gkv487	journal	May 2015
Genome evolution in yeasts Dujon, Bernard; Sherman, David; Fischer, Gilles Nature, Vol. 430, Issue 6995 https://doi.org/10.1038/nature02579	journal	July 2004
SimpleSynteny: a web-based tool for visualization of microsynteny across multiple species Veltri, Daniel; Wight, Martha Malapi; Crouch, Jo Anne Nucleic Acids Research, Vol. 44, Issue W1 https://doi.org/10.1093/nar/gkw330	journal	May 2016
Draft Genome Sequence of a European Isolate of the Apple Canker Pathogen Neonectria ditissima Gómez-Cortecero, Antonio; Harrison, Richard J.; Armitage, Andrew D. Genome Announcements, Vol. 3, Issue 6 https://doi.org/10.1128/genomeA.01243-15	journal	November 2015
The Rhinella arenarum transcriptome: de novo assembly, annotation and gene prediction Ceschin, Danilo Guillermo; Pires, Natalia Susana; Mardirosian, Mariana Noelia Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-57961-4	journal	January 2020
Small genome of the fungus Escovopsis weberi , a specialized disease agent of ant agriculture de Man, Tom J. B.; Stajich, Jason E.; Kubicek, Christian P. Proceedings of the National Academy of Sciences, Vol. 113, Issue 13 https://doi.org/10.1073/pnas.1518501113	journal	March 2016
Phylogeny and taxonomic revision of Thelonectria discophora (Ascomycota, Hypocreales, Nectriaceae) species complex Salgado-Salazar, Catalina; Rossman, Amy Y.; Samuels, Gary J. Fungal Diversity, Vol. 70, Issue 1 https://doi.org/10.1007/s13225-014-0280-y	journal	February 2014
Heading for disaster: Fusarium graminearum on cereal crops: Fusarium graminearum on cereal crops Goswami, Rubella S.; Kistler, H. Corby Molecular Plant Pathology, Vol. 5, Issue 6 https://doi.org/10.1111/j.1364-3703.2004.00252.x	journal	November 2004
A universal classification of eukaryotic transposable elements implemented in Repbase Kapitonov, Vladimir V.; Jurka, Jerzy Nature Reviews Genetics, Vol. 9, Issue 5 https://doi.org/10.1038/nrg2165-c1	journal	May 2008
Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing de Jonge, R.; Peter van Esse, H.; Maruthachalam, K. Proceedings of the National Academy of Sciences, Vol. 109, Issue 13 https://doi.org/10.1073/pnas.1119623109	journal	March 2012
LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach Malapi-Wight, Martha; Demers, Jill E.; Veltri, Daniel Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep26140	journal	May 2016
Draft Genome Sequence of Botrytis cinerea BcDW1, Inoculum for Noble Rot of Grape Berries Blanco-Ulate, B.; Allen, G.; Powell, A. L. T. Genome Announcements, Vol. 1, Issue 3 https://doi.org/10.1128/genomeA.00252-13	journal	May 2013
The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation Mavromatis, Konstantinos; Land, Miriam L.; Brettin, Thomas S. PLoS ONE, Vol. 7, Issue 12 https://doi.org/10.1371/journal.pone.0048837	journal	December 2012
The complete mitochondrial genome of Fusarium oxysporum: Insights into fungal mitochondrial evolution Pantou, Malena P.; Kouvelis, Vassili N.; Typas, Milton A. Gene, Vol. 419, Issue 1-2 https://doi.org/10.1016/j.gene.2008.04.009	journal	August 2008
SECRETOOL: integrated secretome analysis tool for fungi Cortázar, Ana R.; Aransay, Ana M.; Alfaro, Manuel Amino Acids, Vol. 46, Issue 2 https://doi.org/10.1007/s00726-013-1649-z	journal	December 2013
Repeated, Selection-Driven Genome Reduction of Accessory Genes in Experimental Populations Lee, Ming-Chun; Marx, Christopher J. PLoS Genetics, Vol. 8, Issue 5 https://doi.org/10.1371/journal.pgen.1002651	journal	May 2012
Characterization and phylogenetic analysis of the complete mitochondrial genome of the pathogenic fungus Ilyonectria destructans Androsiuk, Piotr; Okorski, Adam; Paukszto, Łukasz Scientific Reports, Vol. 12, Issue 1 https://doi.org/10.1038/s41598-022-05428-z	journal	February 2022
Economic and Social Impacts of Fusarium Head Blight: Changing Farms and Rural Communities in the Northern Great Plains Windels, Carol E. Phytopathology, Vol. 90, Issue 1 https://doi.org/10.1094/PHYTO.2000.90.1.17	journal	January 2000
The Fusarium graminearum Genome Reveals a Link Between Localized Polymorphism and Pathogen Specialization Cuomo, C. A.; Guldener, U.; Xu, J. -R. Science, Vol. 317, Issue 5843 https://doi.org/10.1126/science.1143708	journal	September 2007
Comparative Genomics Analysis of Trichoderma reesei Strains Koike, Hideaki; Aerts, Andrea; LaButti, Kurt Industrial Biotechnology, Vol. 9, Issue 6 https://doi.org/10.1089/ind.2013.0015	journal	December 2013
BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs Simão, Felipe A.; Waterhouse, Robert M.; Ioannidis, Panagiotis Bioinformatics, Vol. 31, Issue 19 https://doi.org/10.1093/bioinformatics/btv351	journal	June 2015
dbCAN: a web resource for automated carbohydrate-active enzyme annotation Yin, Yanbin; Mao, Xizeng; Yang, Jincai Nucleic Acids Research, Vol. 40, Issue W1 https://doi.org/10.1093/nar/gks479	journal	May 2012
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models Stamatakis, Alexandros Bioinformatics, Vol. 22, Issue 21 https://doi.org/10.1093/bioinformatics/btl446	journal	August 2006
Identification and analysis of in planta expressed genes of Magnaporthe oryzae Kim, Soonok; Park, Jongsun; Park, Sook-Young BMC Genomics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2164-11-104	journal	January 2010
Generic concepts in Nectriaceae Lombard, L.; van der Merwe, N. A.; Groenewald, J. Z. Studies in Mycology, Vol. 80 https://doi.org/10.1016/j.simyco.2014.12.002	journal	March 2015
BLAST+: architecture and applications Camacho, Christiam; Coulouris, George; Avagyan, Vahram BMC Bioinformatics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2105-10-421	journal	January 2009
First Report of Volutella Blight caused by Pseudonectria buxi on Japanese Boxwood ( Buxus microphylla ) in Italy Garibaldi, A.; Bertetti, D.; Ortu, G. Plant Disease, Vol. 100, Issue 7 https://doi.org/10.1094/PDIS-09-15-1027-PDN	journal	July 2016
Indifferent, Affectionate, or Deceitful: Lifestyles and Secretomes of Fungi Lowe, Rohan G. T.; Howlett, Barbara J. PLoS Pathogens, Vol. 8, Issue 3 https://doi.org/10.1371/journal.ppat.1002515	journal	March 2012
Model of Cation Transportation Mediated by High-Affinity Potassium Transporters (HKTs) in Higher Plants Su, Yi; Luo, Weigui; Lin, Wanhuang Biological Procedures Online, Vol. 17, Issue 1 https://doi.org/10.1186/s12575-014-0013-3	journal	January 2015
Cladosporium fulvum Avr4 Protects Fungal Cell Walls Against Hydrolysis by Plant Chitinases Accumulating During Infection van den Burg, Harrold A.; Harrison, Stuart J.; Joosten, Matthieu H. A. J. Molecular Plant-Microbe Interactions, Vol. 19, Issue 12 https://doi.org/10.1094/mpmi-19-1420	journal	December 2006
The rapid generation of mutation data matrices from protein sequences Jones, David T.; Taylor, William R.; Thornton, Janet M. Bioinformatics, Vol. 8, Issue 3 https://doi.org/10.1093/bioinformatics/8.3.275	journal	January 1992
Genome sequence of Valsa canker pathogens uncovers a potential adaptation of colonization of woody bark Yin, Zhiyuan; Liu, Huiquan; Li, Zhengpeng New Phytologist, Vol. 208, Issue 4 https://doi.org/10.1111/nph.13544	journal	July 2015
A unified classification system for eukaryotic transposable elements Wicker, Thomas; Sabot, François; Hua-Van, Aurélie Nature Reviews Genetics, Vol. 8, Issue 12 https://doi.org/10.1038/nrg2165	journal	December 2007
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro Molecular Biology and Evolution, Vol. 33, Issue 7 https://doi.org/10.1093/molbev/msw054	journal	March 2016
Fungal evolution: the case of the vanishing mitochondrion Bullerwell, Charles E.; Lang, B. Franz Current Opinion in Microbiology, Vol. 8, Issue 4 https://doi.org/10.1016/j.mib.2005.06.009	journal	August 2005
First Report of Ilyonectria macrodidyma Causing Root Rot of Olive Trees ( Olea europaea ) in California Úrbez-Torres, J. R.; Peduto, F.; Gubler, W. D. Plant Disease, Vol. 96, Issue 9 https://doi.org/10.1094/PDIS-04-12-0330-PDN	journal	September 2012
Genetic basis for the establishment of endosymbiosis in Paramecium He, Ming; Wang, Jinfeng; Fan, Xinpeng The ISME Journal, Vol. 13, Issue 5 https://doi.org/10.1038/s41396-018-0341-4	journal	January 2019
Sarcococca Blight: Use of Whole-Genome Sequencing for Fungal Plant Disease Diagnosis Malapi-Wight, Martha; Salgado-Salazar, Catalina; Demers, Jill E. Plant Disease, Vol. 100, Issue 6 https://doi.org/10.1094/PDIS-10-15-1159-RE	journal	June 2016
First Report of Root Rot Caused by Ilyonectria (=Neonectria) macrodidyma on Avocado (Persea americana) in Italy: Neonectria Root Rot on Avocado in Italy Vitale, Alessandro; Aiello, Dalia; Guarnaccia, Vladimiro Journal of Phytopathology, Vol. 160, Issue 3 https://doi.org/10.1111/j.1439-0434.2011.01869.x	journal	December 2011

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