The Chloroplast Tat Pathway Utilizes the Transmembrane Electric Potential as an Energy Source
Abstract
Here, the thylakoid membrane, located inside the chloroplast, requires proteins transported across it for plastid biogenesis and functional photosynthetic electron transport. The chloroplast Tat translocator found on thylakoids transports proteins from the plastid stroma to the thylakoid lumen. Previous studies have shown that the chloroplast Tat pathway is independent of NTP hydrolysis as an energy source and instead depends on the thylakoid transmembrane proton gradient to power protein translocation. Because of its localization on the same membrane as the proton motive force-dependent F0F1 ATPase, we believed that the chloroplast Tat pathway also made use of the thylakoid electric potential for transporting substrates. By adjusting the rate of photosynthetic proton pumping and by utilizing ionophores, we show that the chloroplast Tat pathway can also utilize the transmembrane electric potential for protein transport. Our findings indicate that the chloroplast Tat pathway is likely dependent on the total protonmotive force (PMF) as an energy source. As a protonmotive-dependent device, certain predictions can be made about structural features expected to be found in the Tat translocon, specifically, the presence of a proton well, a device in the membrane that converts electrical potential into chemical potential.
- Authors:
-
- Univ. of California, Davis, CA (United States). Dept. of Plant Biology
- Publication Date:
- Research Org.:
- Univ. of California, Davis, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
- OSTI Identifier:
- 1490449
- Grant/Contract Number:
- FG02-03ER15405
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Biophysical Journal
- Additional Journal Information:
- Journal Volume: 93; Journal Issue: 6; Journal ID: ISSN 0006-3495
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Braun, Nikolai A., Davis, Andrew W., and Theg, Steven M. The Chloroplast Tat Pathway Utilizes the Transmembrane Electric Potential as an Energy Source. United States: N. p., 2009.
Web. doi:10.1529/biophysj.106.098731.
Braun, Nikolai A., Davis, Andrew W., & Theg, Steven M. The Chloroplast Tat Pathway Utilizes the Transmembrane Electric Potential as an Energy Source. United States. https://doi.org/10.1529/biophysj.106.098731
Braun, Nikolai A., Davis, Andrew W., and Theg, Steven M. 2009.
"The Chloroplast Tat Pathway Utilizes the Transmembrane Electric Potential as an Energy Source". United States. https://doi.org/10.1529/biophysj.106.098731. https://www.osti.gov/servlets/purl/1490449.
@article{osti_1490449,
title = {The Chloroplast Tat Pathway Utilizes the Transmembrane Electric Potential as an Energy Source},
author = {Braun, Nikolai A. and Davis, Andrew W. and Theg, Steven M.},
abstractNote = {Here, the thylakoid membrane, located inside the chloroplast, requires proteins transported across it for plastid biogenesis and functional photosynthetic electron transport. The chloroplast Tat translocator found on thylakoids transports proteins from the plastid stroma to the thylakoid lumen. Previous studies have shown that the chloroplast Tat pathway is independent of NTP hydrolysis as an energy source and instead depends on the thylakoid transmembrane proton gradient to power protein translocation. Because of its localization on the same membrane as the proton motive force-dependent F0F1 ATPase, we believed that the chloroplast Tat pathway also made use of the thylakoid electric potential for transporting substrates. By adjusting the rate of photosynthetic proton pumping and by utilizing ionophores, we show that the chloroplast Tat pathway can also utilize the transmembrane electric potential for protein transport. Our findings indicate that the chloroplast Tat pathway is likely dependent on the total protonmotive force (PMF) as an energy source. As a protonmotive-dependent device, certain predictions can be made about structural features expected to be found in the Tat translocon, specifically, the presence of a proton well, a device in the membrane that converts electrical potential into chemical potential.},
doi = {10.1529/biophysj.106.098731},
url = {https://www.osti.gov/biblio/1490449},
journal = {Biophysical Journal},
issn = {0006-3495},
number = 6,
volume = 93,
place = {United States},
year = {Tue Jan 06 00:00:00 EST 2009},
month = {Tue Jan 06 00:00:00 EST 2009}
}
Web of Science
Figures / Tables:
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Figures / Tables found in this record: