Camelina sativa, an oilseed at the nexus between model system and commercial crop

Malik, Meghna R.; Tang, Jihong; Sharma, Nirmala; Burkitt, Claire; Ji, Yuanyuan; Mykytyshyn, Marie; Bohmert-Tatarev, Karen; Peoples, Oliver; Snell, Kristi D.

doi:10.1007/s00299-018-2308-3

Title: Camelina sativa, an oilseed at the nexus between model system and commercial crop

Journal Article · Thu Jun 07 00:00:00 EDT 2018 · Plant Cell Reports

DOI:https://doi.org/10.1007/s00299-018-2308-3· OSTI ID:1489127

Malik, Meghna R. ^[1]; Tang, Jihong ^[2]; Sharma, Nirmala ^[1]; Burkitt, Claire ^[1]; Ji, Yuanyuan ^[1]; Mykytyshyn, Marie ^[1]; Bohmert-Tatarev, Karen ^[2]; Peoples, Oliver ^[2]; Snell, Kristi D. ^[2]

Metabolix Oilseeds, Inc., Saskatoon (Canada)
Yield10 Bioscience, Inc., Woburn, MA (United States)

The rapid assessment of metabolic engineering strategies in plants is aided by crops that provide simple, high throughput transformation systems, a sequenced genome, and the ability to evaluate the resulting plants in field trials. Camelina sativa provides all of these attributes in a robust oilseed platform. The ability to perform field evaluation of Camelina is a useful, and in some studies essential benefit that allows researchers to evaluate how traits perform outside the strictly controlled conditions of a greenhouse. In the field the plants are subjected to higher light intensities, seasonal diurnal variations in temperature and light, competition for nutrients, and watering regimes dictated by natural weather patterns, all which may affect trait performance. There are difficulties associated with the use of Camelina. The current genetic resources available for Camelina pale in comparison to those developed for the model plant Arabidopsis thaliana; however, the sequence similarity of the Arabidopsis and Camelina genomes often allows the use of Arabidopsis as a reference when additional information is needed. Camelina’s genome, an allohexaploid, is more complex than other model crops, but the diploid inheritance of its three subgenomes is straightforward. The need to navigate three copies of each gene in genome editing or mutagenesis experiments adds some complexity but also provides advantages for gene dosage experiments. In conclusion, the ability to quickly engineer Camelina with novel traits, advance generations, and bulk up homozygous lines for small-scale field tests in less than a year, in our opinion, far outweighs the complexities associated with the crop.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Yield10 Bioscience, Inc., Woburn, MA (United States)

Sponsoring Organization:: USDOE Advanced Research Projects Agency - Energy (ARPA-E)

Grant/Contract Number:: EE0007003

OSTI ID:: 1489127

Journal Information:: Plant Cell Reports, Vol. 37, Issue 10; ISSN 0721-7714

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 32 works

Citation information provided by
Web of Science

References (74)

Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds Nguyen, Huu Tam; Park, Hyunwoo; Koster, Karen L. Plant Biotechnology Journal, Vol. 13, Issue 1 https://doi.org/10.1111/pbi.12233	journal	July 2014
Camelina uses, genetics, genomics, production, and management Berti, Marisol; Gesch, Russ; Eynck, Christina Industrial Crops and Products, Vol. 94 https://doi.org/10.1016/j.indcrop.2016.09.034	journal	December 2016
Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa Morineau, Céline; Bellec, Yannick; Tellier, Frédérique Plant Biotechnology Journal, Vol. 15, Issue 6 https://doi.org/10.1111/pbi.12671	journal	April 2017
De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing Liang, Chao; Liu, Xuan; Yiu, Siu-Ming BMC Genomics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2164-14-146	journal	January 2013
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein Shaner, Nathan C.; Campbell, Robert E.; Steinbach, Paul A. Nature Biotechnology, Vol. 22, Issue 12 https://doi.org/10.1038/nbt1037	journal	November 2004
Camelina as a sustainable oilseed crop: Contributions of plant breeding and genetic engineering Vollmann, Johann; Eynck, Christina Biotechnology Journal, Vol. 10, Issue 4 https://doi.org/10.1002/biot.201400200	journal	February 2015
Camelina: An Emerging Oilseed Platform for Advanced Biofuels and Bio-Based Materials Iskandarov, Umidjon; Kim, Hae Jin; Cahoon, Edgar B. Plants and BioEnergy, p. 131-140 https://doi.org/10.1007/978-1-4614-9329-7_8	book	November 2013
Camelina sativa: An ideal platform for the metabolic engineering and field production of industrial lipids Bansal, Sunil; Durrett, Timothy P. Biochimie, Vol. 120 https://doi.org/10.1016/j.biochi.2015.06.009	journal	January 2016
The developmental transcriptome atlas of the biofuel crop Camelina sativa Kagale, Sateesh; Nixon, John; Khedikar, Yogendra The Plant Journal, Vol. 88, Issue 5 https://doi.org/10.1111/tpj.13302	journal	December 2016
Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop Ruiz-Lopez, Noemi; Haslam, Richard P.; Napier, Johnathan A. The Plant Journal, Vol. 77, Issue 2 https://doi.org/10.1111/tpj.12378	journal	December 2013
Tissue-specific production of limonene in Camelina sativa with the Arabidopsis promoters of genes BANYULS and FRUITFULL Borghi, Monica; Xie, De-Yu Planta, Vol. 243, Issue 2 https://doi.org/10.1007/s00425-015-2425-y	journal	November 2015
Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa Ozseyhan, Mehmet E.; Kang, Jinling; Mu, Xiaopeng Plant Physiology and Biochemistry, Vol. 123 https://doi.org/10.1016/j.plaphy.2017.11.021	journal	February 2018
Engineered GFP as a vital reporter in plants Chiu, Wan-ling; Niwa, Yasuo; Zeng, Weike Current Biology, Vol. 6, Issue 3 https://doi.org/10.1016/S0960-9822(02)00483-9	journal	March 1996
The biology of Canadian weeds. 142. Camelina alyssum (Mill.) Thell.; C. microcarpa Andrz. ex DC.; C. sativa (L.) Crantz. Francis, A.; Warwick, S. I. Canadian Journal of Plant Science, Vol. 89, Issue 4 https://doi.org/10.4141/CJPS08185	journal	July 2009
Constitutive or seed-specific overexpression of Arabidopsis G-protein γ subunit 3 ( AGG3 ) results in increased seed and oil production and improved stress tolerance in Camelina sativa Roy Choudhury, Swarup; Riesselman, Adam J.; Pandey, Sona Plant Biotechnology Journal, Vol. 12, Issue 1 https://doi.org/10.1111/pbi.12115	journal	September 2013
Sowing Date and Tillage Effects on Fall-Seeded Camelina in the Northern Corn Belt Gesch, R. W.; Cermak, S. C. Agronomy Journal, Vol. 103, Issue 4 https://doi.org/10.2134/agronj2010.0485	journal	January 2011
Fluorescent proteins from nonbioluminescent Anthozoa species Matz, Mikhail V.; Fradkov, Arkady F.; Labas, Yulii A. Nature Biotechnology, Vol. 17, Issue 10 https://doi.org/10.1038/13657	journal	October 1999
Tailoring the composition of novel wax esters in the seeds of transgenic Camelina sativa through systematic metabolic engineering Ruiz-Lopez, Noemi; Broughton, Richard; Usher, Sarah Plant Biotechnology Journal, Vol. 15, Issue 7 https://doi.org/10.1111/pbi.12679	journal	February 2017
With a free pass, CRISPR-edited plants reach market in record time Waltz, Emily Nature Biotechnology, Vol. 36, Issue 1 https://doi.org/10.1038/nbt0118-6b	journal	January 2018
Field production, purification and analysis of high-oleic acetyl-triacylglycerols from transgenic Camelina sativa Liu, Jinjie; Tjellström, Henrik; McGlew, Kathleen Industrial Crops and Products, Vol. 65 https://doi.org/10.1016/j.indcrop.2014.11.019	journal	March 2015
Yield Trends Are Insufficient to Double Global Crop Production by 2050 Ray, Deepak K.; Mueller, Nathaniel D.; West, Paul C. PLoS ONE, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pone.0066428	journal	June 2013
Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa ) Gehringer, A.; Friedt, W.; Lühs, W. Genome, Vol. 49, Issue 12 https://doi.org/10.1139/g06-117	journal	December 2006
Metabolic engineering to increase crop yield: From concept to execution Skraly, Frank A.; Ambavaram, Madana M. R.; Peoples, Oliver Plant Science, Vol. 273 https://doi.org/10.1016/j.plantsci.2018.03.011	journal	August 2018
Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism Vollmann, J.; Grausgruber, H.; Stift, G. Plant Breeding, Vol. 124, Issue 5 https://doi.org/10.1111/j.1439-0523.2005.01134.x	journal	October 2005
Over-expression of AtPAP2 in Camelina sativa leads to faster plant growth and higher seed yield Zhang, Youjun; Yu, Laura; Yung, Ka-Fu Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-19	journal	January 2012
Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil Marmon, Sofia; Sturtevant, Drew; Herrfurth, Cornelia Plant Physiology, Vol. 173, Issue 4 https://doi.org/10.1104/pp.16.01865	journal	February 2017
Field trial evaluation of the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Camelina sativa: Making fish oil substitutes in plants Usher, Sarah; Haslam, Richard P.; Ruiz-Lopez, Noemi Metabolic Engineering Communications, Vol. 2 https://doi.org/10.1016/j.meteno.2015.04.002	journal	December 2015
Toward production of jet fuel functionality in oilseeds: identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds Kim, Hae Jin; Silva, Jillian E.; Vu, Hieu Sy Journal of Experimental Botany, Vol. 66, Issue 14 https://doi.org/10.1093/jxb/erv225	journal	May 2015
De novo transcriptome analysis of an imminent biofuel crop, Camelina sativa L. using Illumina GAIIX sequencing platform and identification of SSR markers Mudalkar, Shalini; Golla, Ramesh; Ghatty, Sreenivas Plant Molecular Biology, Vol. 84, Issue 1-2 https://doi.org/10.1007/s11103-013-0125-1	journal	September 2013
Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa An, Dahee; Suh, Mi Chung Plant Biotechnology Reports, Vol. 9, Issue 3 https://doi.org/10.1007/s11816-015-0351-x	journal	May 2015
Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds Abdullah, Hesham M.; Akbari, Parisa; Paulose, Bibin Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0555-5	journal	July 2016
Editing plant genomes with CRISPR/Cas9 Belhaj, Khaoula; Chaparro-Garcia, Angela; Kamoun, Sophien Current Opinion in Biotechnology, Vol. 32 https://doi.org/10.1016/j.copbio.2014.11.007	journal	April 2015
Camelina (Camelina sativa L.) oil as a biofuels feedstock: Golden opportunity or false hope? Moser, Bryan R. Lipid Technology, Vol. 22, Issue 12 https://doi.org/10.1002/lite.201000068	journal	December 2010
Mining and identification of polyunsaturated fatty acid synthesis genes active during camelina seed development using 454 pyrosequencing Wang, Fawei; Chen, Huan; Li, Xiaowei BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0513-6	journal	June 2015
An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds Ruiz-Lopez, Noemi; Haslam, Richard P.; Usher, Sarah Plant Biotechnology Journal, Vol. 13, Issue 9 https://doi.org/10.1111/pbi.12328	journal	January 2015
Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression Chhikara, Sudesh; Abdullah, Hesham M.; Akbari, Parisa Plant Biotechnology Journal, Vol. 16, Issue 5 https://doi.org/10.1111/pbi.12847	journal	November 2017
Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa Aznar-Moreno, J. A.; Durrett, T. P. Plant and Cell Physiology, Vol. 58, Issue 7 https://doi.org/10.1093/pcp/pcx058	journal	April 2017
A reverse glyoxylate shunt to build a non-native route from C4 to C2 in Escherichia coli Mainguet, Samuel E.; Gronenberg, Luisa S.; Wong, Sio Si Metabolic Engineering, Vol. 19 https://doi.org/10.1016/j.ymben.2013.06.004	journal	September 2013
Identification of three genes encoding microsomal oleate desaturases (FAD2) from the oilseed crop Camelina sativa Kang, Jinling; Snapp, Anna R.; Lu, Chaofu Plant Physiology and Biochemistry, Vol. 49, Issue 2 https://doi.org/10.1016/j.plaphy.2010.12.004	journal	February 2011
A dual-site gateway cloning system for simultaneous cloning of two genes for plant transformation Aboulela, Mostafa; Tanaka, Yuji; Nishimura, Kohji Plasmid, Vol. 92 https://doi.org/10.1016/j.plasmid.2017.05.001	journal	July 2017
Advances in genetic improvement of Camelina sativa for biofuel and industrial bio-products Sainger, Manish; Jaiwal, Anjali; Sainger, Poonam Ahlawat Renewable and Sustainable Energy Reviews, Vol. 68 https://doi.org/10.1016/j.rser.2016.10.023	journal	February 2017
Production of mono- and sesquiterpenes in Camelina sativa oilseed Augustin, Jörg M.; Higashi, Yasuhiro; Feng, Xiaohong Planta, Vol. 242, Issue 3, p. 693-708 https://doi.org/10.1007/s00425-015-2367-4	journal	July 2015
Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds Malik, Meghna R.; Yang, Wenyu; Patterson, Nii Plant Biotechnology Journal, Vol. 13, Issue 5 https://doi.org/10.1111/pbi.12290	journal	November 2014
A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa Dalal, Jyoti; Lopez, Harry; Vasani, Naresh B. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0357-1	journal	October 2015
Haploids: Constraints and opportunities in plant breeding Dwivedi, Sangam L.; Britt, Anne B.; Tripathi, Leena Biotechnology Advances, Vol. 33, Issue 6 https://doi.org/10.1016/j.biotechadv.2015.07.001	journal	November 2015
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure Kagale, Sateesh; Koh, Chushin; Nixon, John Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4706	journal	April 2014
Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes Hutcheon, Carolyn; Ditt, Renata F.; Beilstein, Mark BMC Plant Biology, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2229-10-233	journal	January 2010
Short Communication: First report of outcrossing rates in camelina [ Camelina sativa (L.) Crantz], a potential platform for bioindustrial oils Walsh, Kimberly D.; Puttick, Debra M.; Hills, Melissa J. Canadian Journal of Plant Science, Vol. 92, Issue 4 https://doi.org/10.4141/cjps2011-182	journal	July 2012
Green fluorescent protein as a marker for gene expression Chalfie, M.; Tu, Y.; Euskirchen, G. Science, Vol. 263, Issue 5148 https://doi.org/10.1126/science.8303295	journal	February 1994
Combinatorial Effects of Fatty Acid Elongase Enzymes on Nervonic Acid Production in Camelina sativa Huai, Dongxin; Zhang, Yuanyuan; Zhang, Chunyu PLOS ONE, Vol. 10, Issue 6 https://doi.org/10.1371/journal.pone.0131755	journal	June 2015
Synthesis of oleyl oleate wax esters in Arabidopsis thaliana and Camelina sativa seed oil Iven, Tim; Hornung, Ellen; Heilmann, Mareike Plant Biotechnology Journal, Vol. 14, Issue 1 https://doi.org/10.1111/pbi.12379	journal	April 2015
Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape FitzJohn, Richard G.; Armstrong, Tristan T.; Newstrom-Lloyd, Linda E. Euphytica, Vol. 158, Issue 1-2 https://doi.org/10.1007/s10681-007-9444-0	journal	May 2007
Overexpression of Populus×canescens isoprene synthase gene in Camelina sativa leads to alterations in its growth and metabolism Rossi, Lorenzo; Borghi, Monica; Yang, Jinfen Journal of Plant Physiology, Vol. 215 https://doi.org/10.1016/j.jplph.2017.06.005	journal	August 2017
Camelina seed transcriptome: a tool for meal and oil improvement and translational research Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram Plant Biotechnology Journal, Vol. 11, Issue 6 https://doi.org/10.1111/pbi.12068	journal	April 2013
A microspore embryogenesis protocol for Camelina sativa, a multi-use crop Ferrie, A. M. R.; Bethune, T. D. Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 106, Issue 3 https://doi.org/10.1007/s11240-011-9948-0	journal	April 2011
Genome editing for targeted improvement of plants Khandagale, Kiran; Nadaf, Altafhusain Plant Biotechnology Reports, Vol. 10, Issue 6 https://doi.org/10.1007/s11816-016-0417-4	journal	November 2016
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing Jiang, Wen Zhi; Henry, Isabelle M.; Lynagh, Peter G. Plant Biotechnology Journal, Vol. 15, Issue 5 https://doi.org/10.1111/pbi.12663	journal	January 2017
A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis Kourtz, Lauralynn; Dillon, Kevin; Daughtry, Sean Plant Biotechnology Journal, Vol. 3, Issue 4 https://doi.org/10.1111/j.1467-7652.2005.00136.x	journal	July 2005
Overexpression of a synthetic insect–plant geranyl pyrophosphate synthase gene in Camelina sativa alters plant growth and terpene biosynthesis Xi, Jing; Rossi, Lorenzo; Lin, Xiuli Planta, Vol. 244, Issue 1 https://doi.org/10.1007/s00425-016-2504-8	journal	March 2016
Functional analysis of diacylglycerol acyltransferase1 genes from Camelina sativa and effects of CsDGAT1B overexpression on seed mass and storage oil content in C. sativa Kim, Hyojin; Park, Ji Hyun; Kim, Da Jung Plant Biotechnology Reports, Vol. 10, Issue 3 https://doi.org/10.1007/s11816-016-0394-7	journal	May 2016
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases Bae, S.; Park, J.; Kim, J. -S. Bioinformatics, Vol. 30, Issue 10 https://doi.org/10.1093/bioinformatics/btu048	journal	January 2014
Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA Petrie, James R.; Shrestha, Pushkar; Belide, Srinivas PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0085061	journal	January 2014
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation Lu, Chaofu; Kang, Jinling Plant Cell Reports, Vol. 27, Issue 2 https://doi.org/10.1007/s00299-007-0454-0	journal	September 2007
Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa Usher, Sarah; Han, Lihua; Haslam, Richard P. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-06838-0	journal	July 2017
A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa Snapp, Anna R.; Kang, Jinling; Qi, Xiaoli Planta, Vol. 240, Issue 3 https://doi.org/10.1007/s00425-014-2122-2	journal	July 2014
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage Soorni, Jahad; Kazemitabar, Seyed; Kahrizi, Danial Genetika, Vol. 49, Issue 1 https://doi.org/10.2298/GENSR1701173S	journal	January 2017
Transformation of the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple large-scale screening of transformants Liu, Xunjia; Brost, Jennifer; Hutcheon, Carolyn In Vitro Cellular & Developmental Biology - Plant, Vol. 48, Issue 5 https://doi.org/10.1007/s11627-012-9459-7	journal	September 2012
The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit Murugan, Karthik; Babu, Kesavan; Sundaresan, Ramya Molecular Cell, Vol. 68, Issue 1 https://doi.org/10.1016/j.molcel.2017.09.007	journal	October 2017
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR Haeussler, Maximilian; Schönig, Kai; Eckert, Hélène Genome Biology, Vol. 17, Issue 1 https://doi.org/10.1186/s13059-016-1012-2	journal	July 2016
Accumulation of medium-chain, saturated fatty acyl moieties in seed oils of transgenic Camelina sativa Hu, Zhaohui; Wu, Qian; Dalal, Jyoti PLOS ONE, Vol. 12, Issue 2 https://doi.org/10.1371/journal.pone.0172296	journal	February 2017
The upstream domain of soybean oleosin genes contains regulatory elements similar to those of legume storage proteins Rowley, Daniel L.; Herman, Eliot M. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 1345, Issue 1 https://doi.org/10.1016/S0005-2760(97)00004-0	journal	March 1997
Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina Li, Maoyin; Wei, Fang; Tawfall, Amanda Plant Biotechnology Journal, Vol. 13, Issue 6 https://doi.org/10.1111/pbi.12304	journal	December 2014
Efficient generation of mouse models with the prime editing system Liu, Yao; Li, Xiangyang; He, Siting Cell Discovery, Vol. 6, Issue 1 https://doi.org/10.1038/s41421-020-0165-z	journal	April 2020