Porous artificial bone scaffold synthesized from a facile in situ hydroxyapatite coating and crosslinking reaction of crystalline nanocellulose

Huang, Chen; Hao, Naijia; Bhagia, Samarthya; Li, Mi; Meng, Xianzhi; Pu, Yunqiao Joseph; Yong, Qiang; Ragauskas, Arthur J.

doi:10.1016/j.mtla.2018.09.008

Title: Porous artificial bone scaffold synthesized from a facile in situ hydroxyapatite coating and crosslinking reaction of crystalline nanocellulose

Journal Article · Thu Sep 20 00:00:00 EDT 2018 · Materialia

DOI:https://doi.org/10.1016/j.mtla.2018.09.008· OSTI ID:1489096

Huang, Chen ^[1]; Hao, Naijia ^[2]; Bhagia, Samarthya ^[2];

^[2]; Meng, Xianzhi ^[2];

^[3]; Yong, Qiang ^[4];

^[5]

Nanjing Forestry Univ., Nanjing (China); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Nanjing Forestry Univ., Nanjing (China)
Univ. of Tennessee, Knoxville, TN (United States); Univ. of Tennessee Institute of Agriculture, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Research into artificial bone scaffolds has increased substantially over the past decade as current solutions have significant limitations. Inspired by mineral hydroxyapatite (HAP) in natural bone, this study developed a facile in situ HAP coating on cellulose nanocrystals (CNCs) matrix followed by a crosslinking reaction. By changing the amount of CNCs added to a simulated body fluid (SBF), HAP content in the nanocomposite could be controlled between 0 and 40.1%, with a HAP coating thickness of ~10 nm. Moreover, CNCs/HAP was crosslinked with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and polyethylene glycol (PEG) to enhance its water stability and mechanical properties. FTIR and NMR analysis revealed that crosslinking happened via an esterification reaction between CNCs, HAP, PMVEMA, and PEG. Compression strength of the scaffolds showed a result as high as 41.8 MPa, almost 20 times of scaffold prepared by just mixing CNCs and HAP. Further investigations revealed that this scaffold was highly porous (as high as 91.0%) and lightweight (with a density between 60-70 mg/cm³). In addition, this composite showed good biocompatibility as it can stabilize BSA protein, suggesting a promising material as a bone scaffold.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1489096

Journal Information:: Materialia, Vol. 4, Issue C; ISSN 2589-1529

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (38)

New Approach to Bone Tissue Engineering: Simultaneous Application of Hydroxyapatite and Bioactive Glass Coated on a Poly( l -lactic acid) Scaffold Dinarvand, Peyman; Seyedjafari, Ehsan; Shafiee, Abbas ACS Applied Materials & Interfaces, Vol. 3, Issue 11 https://doi.org/10.1021/am201212u	journal	October 2011
Synthesis and biocompatibility evaluation of cellulose/hydroxyapatite nanocomposite scaffold in 1-n-allyl-3-methylimidazolium chloride Zadegan, S.; Hosainalipour, M.; Rezaie, H. R. Materials Science and Engineering: C, Vol. 31, Issue 5 https://doi.org/10.1016/j.msec.2011.02.021	journal	July 2011
Biomimetic nanofibrous scaffolds for bone tissue engineering Holzwarth, Jeremy M.; Ma, Peter X. Biomaterials, Vol. 32, Issue 36 https://doi.org/10.1016/j.biomaterials.2011.09.009	journal	December 2011
Design and Synthesis of Biomimetic Multicomponent All-Bone-Minerals Bionanocomposites Biswas, Abhijit; Bayer, Ilker S.; Zhao, He Biomacromolecules, Vol. 11, Issue 10 https://doi.org/10.1021/bm1009359	journal	October 2010
Synthesis methods for nanosized hydroxyapatite with diverse structures Sadat-Shojai, Mehdi; Khorasani, Mohammad-Taghi; Dinpanah-Khoshdargi, Ehsan Acta Biomaterialia, Vol. 9, Issue 8 https://doi.org/10.1016/j.actbio.2013.04.012	journal	August 2013
Oriented hydroxyapatite in turkey tendon mineralized via the polymer-induced liquid-precursor (PILP) process Jee, Sang Soo; Kasinath, Rajendra Kumar; DiMasi, Elaine CrystEngComm, Vol. 13, Issue 6, p. 2077-2083 https://doi.org/10.1039/c0ce00605j	journal	January 2011
Hierarchical Microspheres Constructed from Chitin Nanofibers Penetrated Hydroxyapatite Crystals for Bone Regeneration Duan, Bo; Shou, Kangquan; Su, Xiaojuan Biomacromolecules, Vol. 18, Issue 7 https://doi.org/10.1021/acs.biomac.7b00408	journal	June 2017
Effect of nano- and micro-hydroxyapatite/chitosan-gelatin network film on human gastric cancer cells Li, Junjie; Yin, Yuji; Yao, Fanglian Materials Letters, Vol. 62, Issue 17-18 https://doi.org/10.1016/j.matlet.2008.02.072	journal	June 2008
In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds Kim, Sinae; Kim, Sang-Soo; Lee, Soo-Hong Biomaterials, Vol. 29, Issue 8 https://doi.org/10.1016/j.biomaterials.2007.11.005	journal	March 2008
Biomimetic composite scaffolds based mineralization of hydroxyapatite on electrospun calcium-containing poly(vinyl alcohol) nanofibers Chang, Wenkai; Mu, Xueyan; Zhu, Xiaoqun Materials Science and Engineering: C, Vol. 33, Issue 7 https://doi.org/10.1016/j.msec.2013.06.023	journal	October 2013
Chitosan(PEO)/silica hybrid nanofibers as a potential biomaterial for bone regeneration Toskas, Georgios; Cherif, Chokri; Hund, Rolf-Dieter Carbohydrate Polymers, Vol. 94, Issue 2 https://doi.org/10.1016/j.carbpol.2013.01.068	journal	May 2013
Evaluation of the mechanical properties, in vitro biodegradability and cytocompatibility of natural chitosan/hydroxyapatite/nano-Fe3O4 composite Heidari, Fatemeh; Razavi, Mehdi; Bahrololoom, Mohammad E. Ceramics International, Vol. 44, Issue 1 https://doi.org/10.1016/j.ceramint.2017.09.170	journal	January 2018
Mesoporous silica formation on hydroxyapatite nanoparticles Yamada, Shota; Nishikawa, Masami; Tagaya, Motohiro Materials Letters, Vol. 211 https://doi.org/10.1016/j.matlet.2017.10.009	journal	January 2018
Review of Hydrogels and Aerogels Containing Nanocellulose De France, Kevin J.; Hoare, Todd; Cranston, Emily D. Chemistry of Materials, Vol. 29, Issue 11 https://doi.org/10.1021/acs.chemmater.7b00531	journal	April 2017
Preparation and Properties of Bamboo Fiber/Nano-hydroxyapatite/Poly(lactic- co -glycolic) Composite Scaffold for Bone Tissue Engineering Jiang, Liuyun; Li, Ye; Xiong, Chengdong ACS Applied Materials & Interfaces, Vol. 9, Issue 5 https://doi.org/10.1021/acsami.6b15032	journal	January 2017
Biomimetic composite scaffolds based on mineralization of hydroxyapatite on electrospun poly(ɛ-caprolactone)/nanocellulose fibers Si, Junhui; Cui, Zhixiang; Wang, Qianting Carbohydrate Polymers, Vol. 143 https://doi.org/10.1016/j.carbpol.2016.02.015	journal	June 2016
Fabrication of nanocellulose–hydroxyapatite composites and their application as water-resistant transparent coatings Ishikawa, Mai; Oaki, Yuya; Tanaka, Yoshihisa Journal of Materials Chemistry B, Vol. 3, Issue 28 https://doi.org/10.1039/C5TB00927H	journal	January 2015
NIH Image to ImageJ: 25 years of image analysis Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W. Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2089	journal	June 2012
Preparation and properties of nano-hydroxyapatite/chitosan/carboxymethyl cellulose composite scaffold Jiang, Liuyun; Li, Yubao; Wang, Xuejiang Carbohydrate Polymers, Vol. 74, Issue 3 https://doi.org/10.1016/j.carbpol.2008.04.035	journal	November 2008
Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering Wei, Guobao; Ma, Peter X. Biomaterials, Vol. 25, Issue 19 https://doi.org/10.1016/j.biomaterials.2003.12.005	journal	August 2004
Cellulose Nanocrystals: Chemistry, Self-Assembly, and Applications Habibi, Youssef; Lucia, Lucian A.; Rojas, Orlando J. Chemical Reviews, Vol. 110, Issue 6 https://doi.org/10.1021/cr900339w	journal	June 2010
A novel nanocomposite film prepared from crosslinked cellulosic whiskers Goetz, Lee; Mathew, Aji; Oksman, Kristiina Carbohydrate Polymers, Vol. 75, Issue 1 https://doi.org/10.1016/j.carbpol.2008.06.017	journal	January 2009
Poly(methyl vinyl ether- co -maleic acid)−Polyethylene Glycol Nanocomposites Cross-Linked In Situ with Cellulose Nanowhiskers Goetz, Lee; Foston, Marcus; Mathew, Aji P. Biomacromolecules, Vol. 11, Issue 10 https://doi.org/10.1021/bm1006695	journal	October 2010
Poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as innate immune system activators Camacho, A. I.; Da Costa Martins, R.; Tamayo, I. Vaccine, Vol. 29, Issue 41 https://doi.org/10.1016/j.vaccine.2011.05.072	journal	September 2011
Influence of molecular weight of PEG chain on interaction between streptavidin and biotin–PEG-conjugated phospholipids studied with QCM-D Teramura, Yuji; Kuroyama, Kohei; Takai, Madoka Acta Biomaterialia, Vol. 30 https://doi.org/10.1016/j.actbio.2015.11.003	journal	January 2016
Synthesis and characterization of hydroxyapatite crystals: A review study on the analytical methods Koutsopoulos, S. Journal of Biomedical Materials Research, Vol. 62, Issue 4 https://doi.org/10.1002/jbm.10280	journal	September 2002
Identification of Cellulosic Fibres by FTIR Spectroscopy - Thread and Single Fibre Analysis by Attenuated Total Reflectance Garside, Paul; Wyeth, Paul Studies in Conservation, Vol. 48, Issue 4 https://doi.org/10.1179/sic.2003.48.4.269	journal	December 2003
Co-production of bio-ethanol, xylonic acid and slow-release nitrogen fertilizer from low-cost straw pulping solid residue Huang, Chen; Ragauskas, Arthur J.; Wu, Xinxing Bioresource Technology, Vol. 250 https://doi.org/10.1016/j.biortech.2017.11.060	journal	February 2018
Effect of pH on the Carbonate Incorporation into the Hydroxyapatite Prepared by an Oxidative Decomposition of Calcium-EDTA Chelate: Effect of pH on the Carbonate Incorporation Yusufoglu, Yusuf; Akinc, Mufit Journal of the American Ceramic Society, Vol. 91, Issue 1 https://doi.org/10.1111/j.1551-2916.2007.02092.x	journal	December 2007
Microstructural and mechanical properties of porous biocomposite scaffolds based on polyvinyl alcohol, nano-hydroxyapatite and cellulose nanocrystals Kumar, Anuj; Negi, Yuvraj Singh; Choudhary, Veena Cellulose, Vol. 21, Issue 5 https://doi.org/10.1007/s10570-014-0339-7	journal	July 2014
Crosslinked PVA nanofibers reinforced with cellulose nanocrystals: Water interactions and thermomechanical properties Peresin, Maria Soledad; Vesterinen, Arja-Helena; Habibi, Youssef Journal of Applied Polymer Science, Vol. 131, Issue 11 https://doi.org/10.1002/app.40334	journal	January 2014
Development of facture free clay-based aerogel: Formulation and architectural mechanisms Huang, Pei; Fan, Mizi Composites Part B: Engineering, Vol. 91 https://doi.org/10.1016/j.compositesb.2016.01.058	journal	April 2016
The Microstructure of Cellulose Nanocrystal Aerogels as Revealed by Transmission Electron Microscope Tomography Buesch, Christian; Smith, Sean W.; Eschbach, Peter Biomacromolecules, Vol. 17, Issue 9 https://doi.org/10.1021/acs.biomac.6b00764	journal	August 2016
Chemically Cross-Linked Cellulose Nanocrystal Aerogels with Shape Recovery and Superabsorbent Properties Yang, Xuan; Cranston, Emily D. Chemistry of Materials, Vol. 26, Issue 20 https://doi.org/10.1021/cm502873c	journal	October 2014
Porous stable poly(lactic acid)/ethyl cellulose/hydroxyapatite composite scaffolds prepared by a combined method for bone regeneration Mao, Daoyong; Li, Qing; Bai, Ningning Carbohydrate Polymers, Vol. 180 https://doi.org/10.1016/j.carbpol.2017.10.031	journal	January 2018
Characterization of novel akermanite:poly-ϵ-caprolactone scaffolds for human adipose-derived stem cells bone tissue engineering: Akermanite:poly-ϵ-caprolactone scaffolds in stem cell bone tissue engineering Zanetti, As; McCandless, Gt; Chan, Jy Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, Issue 4 https://doi.org/10.1002/term.1646	journal	November 2012
Biofabrication of a PLGA-TCP-based porous bioactive bone substitute with sustained release of icaritin: Biofabrication of a PLGA/TCP-based bone substitute Xie, Xin-Hui; Wang, Xin-Luan; Zhang, Ge Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, Issue 8 https://doi.org/10.1002/term.1679	journal	December 2012
Mechanical properties and the hierarchical structure of bone Rho, Jae-Young; Kuhn-Spearing, Liisa; Zioupos, Peter Medical Engineering & Physics, Vol. 20, Issue 2 https://doi.org/10.1016/s1350-4533(98)00007-1	journal	March 1998

Cited By (2)

Biomimetic composite scaffold from an in situ hydroxyapatite coating on cellulose nanocrystals Huang, Chen; Bhagia, Samarthya; Hao, Naijia RSC Advances, Vol. 9, Issue 10 https://doi.org/10.1039/c8ra09523j	journal	January 2019
The Effect of Hydroxyapatite Prepared by In Situ Synthesis on the Properties of Poly(Vinyl Alcohol)/Cellulose Nanocrystals Biomaterial Panyasiri, Panee; Lam, Nga Tien; Sukyai, Prakit Journal of Polymers and the Environment, Vol. 28, Issue 1 https://doi.org/10.1007/s10924-019-01599-5	journal	October 2019

Linked Research (3)

Similar Records

Biomimetic composite scaffold from an in situ hydroxyapatite coating on cellulose nanocrystals

Journal Article · Fri Feb 15 00:00:00 EST 2019 · RSC Advances · OSTI ID:1489096

Huang, Chen; Bhagia, Samarthya; Hao, Naijia; +4 more

Bio-inspired nanocomposite by layer-by-layer coating of chitosan/hyaluronic acid multilayers on a hard nanocellulose-hydroxyapatite matrix

Journal Article · Fri Jun 28 00:00:00 EDT 2019 · Carbohydrate Polymers · OSTI ID:1489096

Huang, Chen; Fang, Guigan; Zhao, Yangyang; +4 more

Synergistic enhancement of nanocellulose foam with dual in situ mineralization and crosslinking reaction

Journal Article · Sat Oct 24 00:00:00 EDT 2020 · International Journal of Biological Macromolecules · OSTI ID:1489096

Huang, Chen; Zhan, Yunni; Hao, Xin; +5 more

Related Subjects

60 APPLIED LIFE SCIENCES
Cellulose nanocrystals
Hydroxyapatite
Crosslinking
Strong mechanical property
Bone scaffold

Data for: Porous artificial bone scaffold synthesized from a facile in situ hydroxyapatite coating and crosslinking reaction of crystalline nanocellulose Ragauskas, Arthur https://doi.org/10.17632/sbrbsvthbm.1 The current record is supplemented by this dataset	dataset	January 2020
Data for: Porous artificial bone scaffold synthesized from a facile in situ hydroxyapatite coating and crosslinking reaction of crystalline nanocellulose Ragauskas, Arthur https://doi.org/10.17632/vzfgcg9pgc The current record is supplemented by this dataset	dataset	January 2020
Data for: Porous artificial bone scaffold synthesized from a facile in situ hydroxyapatite coating and crosslinking reaction of crystalline nanocellulose Ragauskas, Arthur https://doi.org/10.17632/vzfgcg9pgc.1 The current record is supplemented by this dataset	dataset	January 2020