Post-Fermentation Recovery of Biobased Carboxylic Acids

Karp, Eric M.; Cywar, Robin M.; Manker, Lorenz P.; Saboe, Patrick O.; Nimlos, Claire T.; Salvachúa, Davinia; Wang, Xiaoqing; Black, Brenna A.; Reed, Michelle L.; Michener, William E.; Rorrer, Nicholas A.; Beckham, Gregg T.

doi:10.1021/acssuschemeng.8b03703

Title: Post-Fermentation Recovery of Biobased Carboxylic Acids

Journal Article · Thu Oct 04 00:00:00 EDT 2018 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.8b03703· OSTI ID:1481254

Karp, Eric M. ^[1]; Cywar, Robin M. ^[1]; Manker, Lorenz P. ^[1]; Saboe, Patrick O. ^[1]; Nimlos, Claire T. ^[1]; Salvachúa, Davinia ^[1]; Wang, Xiaoqing ^[1]; Black, Brenna A. ^[1]; Reed, Michelle L. ^[1]; Michener, William E. ^[1]; Rorrer, Nicholas A. ^[1]; Beckham, Gregg T. ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center

Carboxylic acids are common products produced from the bioconversion of renewable feedstocks. In these processes the separation of the acid product from fermentation broth is the most energy and cost intensive unit operation. Thus, the development of robust, scalable separation approaches that can be applied to a variety of carboxylates is of critical importance to the development of processes that utilize carboxylic acids as platform chemicals. Here we report a batch separation method that includes cell and particulate removal, cation exchange, activated carbon treatment, dewatering with a polymer resin, and product recovery. This method is demonstrated on two unique fermentation broths both derived from corn stover hydrolysate to separate neat succinic and propionic acid. For succinic acid, a crystallization yield of 91% with a product purity of 99.93% was achieved. To our knowledge this is the highest reported crystallization yield and purity for the recovery of succinic acid. Additionally, the method requires approximately 50% less energy compared to standard evaporative crystallization approaches. For propionic acid, neat liquid product was obtained with a distillation yield of 80% and purity of 98%. Finally, these excellent results achieved in terms of yield and purity for succinic and propionic acid, two acids with widely different physical properties, from chemically complex hydrolysate broth demonstrates the effective and robust nature of this approach.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1481254

Report Number(s):: NREL/JA-5100-72720

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 11; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 22 works

Citation information provided by
Web of Science

References (40)

Renewable acrylonitrile production Karp, Eric M.; Eaton, Todd R.; Sànchez i. Nogué, Violeta Science, Vol. 358, Issue 6368 https://doi.org/10.1126/science.aan1059	journal	December 2017
Platform biochemicals for a biorenewable chemical industry Nikolau, Basil J.; Perera, M. Ann D. N.; Brachova, Libuse The Plant Journal, Vol. 54, Issue 4 https://doi.org/10.1111/j.1365-313X.2008.03484.x	journal	May 2008
Bridging the Chemical and Biological Catalysis Gap: Challenges and Outlooks for Producing Sustainable Chemicals Schwartz, Thomas J.; O’Neill, Brandon J.; Shanks, Brent H. ACS Catalysis, Vol. 4, Issue 6 https://doi.org/10.1021/cs500364y	journal	April 2014
Coupling chemical and biological catalysis: a flexible paradigm for producing biobased chemicals Schwartz, Thomas J.; Shanks, Brent H.; Dumesic, James A. Current Opinion in Biotechnology, Vol. 38 https://doi.org/10.1016/j.copbio.2015.12.017	journal	April 2016
Unleashing Biocatalysis/Chemical Catalysis Synergies for Efficient Biomass Conversion Shanks, Brent H. ACS Chemical Biology, Vol. 2, Issue 8 https://doi.org/10.1021/cb7001522	journal	August 2007
Next-Generation Catalysis for Renewables: Combining Enzymatic with Inorganic Heterogeneous Catalysis for Bulk Chemical Production Vennestrøm, Peter N. R.; Christensen, Claus H.; Pedersen, Sven ChemCatChem, Vol. 2, Issue 3 https://doi.org/10.1002/cctc.200900248	journal	March 2010
Recovery of carboxylic acids produced by fermentation López-Garzón, Camilo S.; Straathof, Adrie J. J. Biotechnology Advances, Vol. 32, Issue 5 https://doi.org/10.1016/j.biotechadv.2014.04.002	journal	September 2014
Novel resin-based vacuum distillation-crystallisation method for recovery of succinic acid crystals from fermentation broths Lin, Sze Ki Carol; Du, Chenyu; Blaga, Alexandra Cristina Green Chemistry, Vol. 12, Issue 4 https://doi.org/10.1039/b913021g	journal	January 2010
Production of very-high purity succinic acid from fermentation broth using microfiltration and nanofiltration-assisted crystallization Thuy, Nguyen Thi Huong; Boontawan, Apichat Journal of Membrane Science, Vol. 524 https://doi.org/10.1016/j.memsci.2016.11.073	journal	February 2017
Salting-out extraction and crystallization of succinic acid from fermentation broths Sun, Yaqin; Yan, Ling; Fu, Hongxin Process Biochemistry, Vol. 49, Issue 3 https://doi.org/10.1016/j.procbio.2013.12.016	journal	March 2014
Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation Salvachúa, Davinia; Mohagheghi, Ali; Smith, Holly Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0425-1	journal	February 2016
Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici Wang, Xiaoqing; Salvachúa, Davinia; Sànchez i. Nogué, Violeta Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0884-z	journal	August 2017
Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z der Werf, M. J. Van; Guettler, Michael V.; Jain, Mahendra K. Archives of Microbiology, Vol. 167, Issue 6 https://doi.org/10.1007/s002030050452	journal	May 1997
Removal and recovery of organic pollutants from aquatic environment. 4. Separation of carboxylic acids from aqueous solution using crosslinked poly(4-vinylpyridine) Kawabata, Nariyoshi; Yoshida, Jun-ichi; Tanigawa, Yukio Industrial & Engineering Chemistry Product Research and Development, Vol. 20, Issue 2 https://doi.org/10.1021/i300002a030	journal	June 1981
Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate Bradfield, Michael F. A.; Mohagheghi, Ali; Salvachúa, Davinia Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0363-3	journal	November 2015
Sorption of phenolics and carboxylic acids on polybenzimidazole Chanda, M.; O'Driscoll, K. F.; Rempel, G. L. Reactive Polymers, Ion Exchangers, Sorbents, Vol. 4, Issue 1 https://doi.org/10.1016/0167-6989(85)90032-7	journal	December 1985
Solubility Measurement Using Differential Scanning Calorimetry Mohan, Rajeev; Lorenz, Heike; Myerson, Allan S. Industrial & Engineering Chemistry Research, Vol. 41, Issue 19 https://doi.org/10.1021/ie0200353	journal	August 2002
Effective purification of succinic acid from fermentation broth produced by Mannheimia succiniciproducens Huh, Yun Suk; Jun, Young-Si; Hong, Yeon Ki Process Biochemistry, Vol. 41, Issue 6 https://doi.org/10.1016/j.procbio.2006.01.020	journal	June 2006
One step recovery of succinic acid from fermentation broths by crystallization Li, Qiang; Wang, Dan; Wu, Yong Separation and Purification Technology, Vol. 72, Issue 3, p. 294-300 https://doi.org/10.1016/j.seppur.2010.02.021	journal	May 2010
Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation-crystallisation method Luque, Rafael; Lin, Carol S. K.; Du, Chenyu Green Chemistry, Vol. 11, Issue 2, p. 193-200 https://doi.org/10.1039/B813409J	journal	January 2009
Adsorption of Lactic Acid on Weak Base Polymeric Resins Dethe, M. J.; Marathe, K. V.; Gaikar, V. G. Separation Science and Technology, Vol. 41, Issue 13 https://doi.org/10.1080/01496390600851384	journal	September 2006
Vapor-Liquid Equilibria of Water-Lower Fatty Acid Systems: Water-Formic Acid, Water Acetic Acid and Water-Propionic Acid. Ito, Tetsuo; Yoshida, Fumitake Journal of Chemical & Engineering Data, Vol. 8, Issue 3 https://doi.org/10.1021/je60018a012	journal	July 1963
The production of propionic acid, propanol and propylene via sugar fermentation: an industrial perspective on the progress, technical challenges and future outlook Rodriguez, Brandon A.; Stowers, Chris C.; Pham, Viet Green Chem., Vol. 16, Issue 3 https://doi.org/10.1039/C3GC42000K	journal	January 2014
Extraction of carboxylic acids with amine extractants. 2. Chemical interactions and interpretation of data Tamada, Janet A.; King, C. Judson Industrial & Engineering Chemistry Research, Vol. 29, Issue 7 https://doi.org/10.1021/ie00103a036	journal	July 1990
Propionic acid production by extractive fermentation. I. Solvent considerations Gu, Zhong; Glatz, Bonita A.; Glatz, Charles E. Biotechnology and Bioengineering, Vol. 57, Issue 4 https://doi.org/10.1002/(SICI)1097-0290(19980220)57:4<454::AID-BIT9>3.0.CO;2-L	journal	February 1998
Membrane processes in biotechnology: An overview Charcosset, Catherine Biotechnology Advances, Vol. 24, Issue 5 https://doi.org/10.1016/j.biotechadv.2006.03.002	journal	September 2006
Comparative analysis of fouling characteristics of ceramic and polymeric microfiltration membranes using filtration models Lee, Seung-Jin; Dilaver, Mehmet; Park, Pyung-Kyu Journal of Membrane Science, Vol. 432 https://doi.org/10.1016/j.memsci.2013.01.013	journal	April 2013
The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass Biddy, Mary J.; Davis, Ryan; Humbird, David ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 6 https://doi.org/10.1021/acssuschemeng.6b00243	journal	May 2016
Microbial production of organic acids: expanding the markets Sauer, Michael; Porro, Danilo; Mattanovich, Diethard Trends in Biotechnology, Vol. 26, Issue 2, p. 100-108 https://doi.org/10.1016/j.tibtech.2007.11.006	journal	February 2008
A Comparison of Continuous Counter-Current Ion Exchange Equipment for Regeneration of Resins Slater, Michael J.; Cooper, Paul F.; Itari, Godfrey B. Chemie Ingenieur Technik - CIT, Vol. 47, Issue 14 https://doi.org/10.1002/cite.330471403	journal	July 1975
Investigation of nanofiltration as a purification step for lactic acid production processes based on conventional and bipolar electrodialysis operations Bouchoux, Antoine; Roux-de Balmann, Hélène; Lutin, Florence Separation and Purification Technology, Vol. 52, Issue 2 https://doi.org/10.1016/j.seppur.2006.05.011	journal	December 2006
Modeling of the separation of lactic acid from an aqueous mixture by adsorption on polyvinylpyridine resin and desorption with methanol Delgado, José Antonio; Águeda, Vicente Ismael; Uguina, María Ángeles Separation and Purification Technology, Vol. 200 https://doi.org/10.1016/j.seppur.2018.02.047	journal	July 2018
In situ recovery of bio-based carboxylic acids Saboe, Patrick O.; Manker, Lorenz P.; Michener, William E. Green Chemistry, Vol. 20, Issue 8 https://doi.org/10.1039/C7GC03747C	journal	January 2018
Advances in in-situ product recovery (ISPR) in whole cell biotechnology during the last decade Van Hecke, Wouter; Kaur, Guneet; De Wever, Heleen Biotechnology Advances, Vol. 32, Issue 7 https://doi.org/10.1016/j.biotechadv.2014.07.003	journal	November 2014
Intensification of enzymatic conversion of glucose to lactic acid by reactive extraction Wasewar, Kailas L.; Pangarkar, Vishwas G.; Heesink, A. Bert M. Chemical Engineering Science, Vol. 58, Issue 15 https://doi.org/10.1016/S0009-2509(03)00221-5	journal	August 2003
Ethanol and isobutanol dehydration by heat-integrated distillation Grisales Díaz, Víctor Hugo; Olivar Tost, Gerard Chemical Engineering and Processing: Process Intensification, Vol. 108 https://doi.org/10.1016/j.cep.2016.07.005	journal	October 2016
In Situ Product Removal as a Tool for Bioprocessing Freeman, Amihay; Woodley, John M.; Lilly, Malcolm D. Nature Biotechnology, Vol. 11, Issue 9 https://doi.org/10.1038/nbt0993-1007	journal	September 1993
Integrated bioprocesses Schügerl, Karl; Hubbuch, Jürgen Current Opinion in Microbiology, Vol. 8, Issue 3 https://doi.org/10.1016/j.mib.2005.01.002	journal	June 2005
Future directions forin-situ product removal (ISPR) Woodley, John M.; Bisschops, Marc; Straathof, Adrie J. J. Journal of Chemical Technology & Biotechnology, Vol. 83, Issue 2 https://doi.org/10.1002/jctb.1790	journal	January 2008
Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate Nelson, Robert; Peterson, Darren; Karp, Eric Fermentation, Vol. 3, Issue 1 https://doi.org/10.3390/fermentation3010010	journal	March 2017

Cited By (3)

In situ product recovery of bio-based ethyl esters via hybrid extraction-distillation Saboe, Patrick O.; Monroe, Hanna R.; Michener, William E. Green Chemistry, Vol. 21, Issue 19 https://doi.org/10.1039/c9gc01844a	journal	January 2019
Tailoring diesel bioblendstock from integrated catalytic upgrading of carboxylic acids: a “fuel property first” approach Huo, Xiangchen; Huq, Nabila A.; Stunkel, Jim Green Chemistry, Vol. 21, Issue 21 https://doi.org/10.1039/c9gc01820d	journal	January 2019
Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability Wendt, Lynn M.; Kinchin, Christopher; Wahlen, Bradley D. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1420-0	journal	April 2019