Two-Color Emitting Colloidal Nanocrystals as Single-Particle Ratiometric Probes of Intracellular pH

Bruni, Francesco; Pedrini, Jacopo; Bossio, Caterina; Santiago-Gonzalez, Beatriz; Meinardi, Francesco; Bae, Wan Ki; Klimov, Victor I.; Lanzani, Guglielmo; Brovelli, Sergio

doi:10.1002/adfm.201605533

Title: Two-Color Emitting Colloidal Nanocrystals as Single-Particle Ratiometric Probes of Intracellular pH

Journal Article · Mon Feb 06 00:00:00 EST 2017 · Advanced Functional Materials

DOI:https://doi.org/10.1002/adfm.201605533· OSTI ID:1481142

Bruni, Francesco ^[1]; Pedrini, Jacopo ^[1]; Bossio, Caterina ^[2]; Santiago-Gonzalez, Beatriz ^[1]; Meinardi, Francesco ^[1]; Bae, Wan Ki ^[3]; Klimov, Victor I. ^[4]; Lanzani, Guglielmo ^[5]; Brovelli, Sergio ^[1]

Univ. degli Studi di Milano-Bicocca, Milano (Italy). Dipartimento di Scienza dei Materiali
Istituto Italiano di Tecnologia, Milano (Italy). Center for Nano Science and Technology
Korea Inst. of Science and Technology, Seoul (Korea, Republic of). Photo-Electronic Hybrids Research Center National Agenda Research Division
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Univ. degli Studi di Milano-Bicocca, Milano (Italy). Dipartimento di Scienza dei Materiali; Politecnico di Milano (Italy)

Intracellular pH is a key parameter in many biological mechanisms and cell metabolism and is used to detect and monitor cancer formation and brain or heart diseases. pH‐sensing is typically performed by fluorescence microscopy using pH‐responsive dyes. Accuracy is limited by the need for quantifying the absolute emission intensity in living biological samples. An alternative with a higher sensitivity and precision uses probes with a ratiometric response arising from the different pH‐sensitivity of two emission channels of a single emitter. Current ratiometric probes are complex constructs suffering from instability and cross‐readout due to their broad emission spectra. Here, we overcome such limitations using a single‐particle ratiometric pH probe based on dot‐in‐bulk CdSe/CdS nanocrystals (NCs). These nanostructures feature two fully‐separated narrow emissions with different pH sensitivity arising from radiative recombination of core‐ and shell‐localized excitons. The core emission is nearly independent of the pH, whereas the shell luminescence increases in the 3–11 pH range, resulting in a cross‐readout‐free ratiometric response as strong as 600%. In vitro microscopy demonstrates that the ratiometric response in biologic media resembles the precalibralation curve obtained through far‐field titration experiments. The NCs show good biocompatibility, enabling us to monitor in real‐time the pH in living cells.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1481142

Alternate ID(s):: OSTI ID: 1400821

Report Number(s):: LA-UR-17-31356

Journal Information:: Advanced Functional Materials, Vol. 27, Issue 12; ISSN 1616-301X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 31 works

Citation information provided by
Web of Science

References (81)

Green and Orange CdTe Quantum Dots as Effective pH-Sensitive Fluorescent Probes for Dual Simultaneous and Independent Detection of Viruses Deng, Zhengtao; Zhang, Yun; Yue, Jiachang The Journal of Physical Chemistry B, Vol. 111, Issue 41 https://doi.org/10.1021/jp074609z	journal	October 2007
Regulation of pH in the mammalian central nervous system under normal and pathological conditions: Facts and hypotheses Obara, Marta; Szeliga, Monika; Albrecht, Jan Neurochemistry International, Vol. 52, Issue 6 https://doi.org/10.1016/j.neuint.2007.10.015	journal	May 2008
Experimental Determination of the Extinction Coefficient of CdTe, CdSe, and CdS Nanocrystals Yu, W. William; Qu, Lianhua; Guo, Wenzhuo Chemistry of Materials, Vol. 15, Issue 14, p. 2854-2860 https://doi.org/10.1021/cm034081k	journal	July 2003
Effect of Core/Shell Interface on Carrier Dynamics and Optical Gain Properties of Dual-Color Emitting CdSe/CdS Nanocrystals Pinchetti, Valerio; Meinardi, Francesco; Camellini, Andrea ACS Nano, Vol. 10, Issue 7 https://doi.org/10.1021/acsnano.6b02635	journal	June 2016
Prospects of Nanoscience with Nanocrystals Kovalenko, Maksym V.; Manna, Liberato; Cabot, Andreu ACS Nano, Vol. 9, Issue 2 https://doi.org/10.1021/nn506223h	journal	January 2015
Core/Shell Fluorescent Silica Nanoparticles for Chemical Sensing: Towards Single-Particle Laboratories Burns, Andrew; Sengupta, Prabuddha; Zedayko, Tara Small, Vol. 2, Issue 6 https://doi.org/10.1002/smll.200600017	journal	June 2006
Polymer microcapsules as mobile local pH-sensors Kreft, Oliver; Javier, Almudena Muñoz; Sukhorukov, Gleb B. Journal of Materials Chemistry, Vol. 17, Issue 42 https://doi.org/10.1039/b705419j	journal	January 2007
Dual emission in asymmetric “giant” PbS/CdS/CdS core/shell/shell quantum dots Zhao, Haiguang; Sirigu, Gianluca; Parisini, Andrea Nanoscale, Vol. 8, Issue 7 https://doi.org/10.1039/C5NR08881J	journal	January 2016
Photostability of thiol-capped CdTe quantum dots in living cells: the effect of photo-oxidation Ma, J.; Chen, Ji-Yao; Guo, J. Nanotechnology, Vol. 17, Issue 9 https://doi.org/10.1088/0957-4484/17/9/002	journal	March 2006
Quantum Dot–Fluorescent Protein FRET Probes for Sensing Intracellular pH Dennis, Allison M.; Rhee, Won Jong; Sotto, David ACS Nano, Vol. 6, Issue 4 https://doi.org/10.1021/nn2038077	journal	March 2012
The role of disturbed pH dynamics and the Na+/H+ exchanger in metastasis Cardone, Rosa A.; Casavola, Valeria; Reshkin, Stephan J. Nature Reviews Cancer, Vol. 5, Issue 10 https://doi.org/10.1038/nrc1713	journal	September 2005
A water-soluble polythiophene–Au nanoparticle composite for pH sensing Panda, Biswa Ranjan; Chattopadhyay, Arun Journal of Colloid and Interface Science, Vol. 316, Issue 2 https://doi.org/10.1016/j.jcis.2007.08.033	journal	December 2007
Bright White-Light Emission from Semiconductor Nanocrystals: by Chance and by Design Sapra, S.; Mayilo, S.; Klar, T. A. Advanced Materials, Vol. 19, Issue 4 https://doi.org/10.1002/adma.200602267	journal	February 2007
Particle-Based Optical Sensing of Intracellular Ions at the Example of Calcium - What Are the Experimental Pitfalls? Kantner, Karsten; Ashraf, Sumaira; Carregal-Romero, Susana Small, Vol. 11, Issue 8 https://doi.org/10.1002/smll.201402110	journal	December 2014
Dual-Color Electroluminescence from Dot-in-Bulk Nanocrystals Brovelli, Sergio; Bae, Wan Ki; Galland, Christophe Nano Letters, Vol. 14, Issue 2 https://doi.org/10.1021/nl403478s	journal	December 2013
Dual Wavelength Electroluminescence from CdSe/CdS Tetrapods Wong, Jen It; Mishra, Nimai; Xing, Guichuan ACS Nano, Vol. 8, Issue 3 https://doi.org/10.1021/nn500030t	journal	March 2014
Synthesis and Application of Colloidal CuInS ₂ Semiconductor Nanocrystals Kolny-Olesiak, Joanna; Weller, Horst ACS Applied Materials & Interfaces, Vol. 5, Issue 23 https://doi.org/10.1021/am404084d	journal	November 2013
Imaging intracellular pH in a reef coral and symbiotic anemone Venn, A. A.; Tambutte, E.; Lotto, S. Proceedings of the National Academy of Sciences, Vol. 106, Issue 39 https://doi.org/10.1073/pnas.0902894106	journal	August 2009
Intracellular pH regulation in heart Vaughan-Jones, Richard D.; Spitzer, Kenneth W.; Swietach, Pawel Journal of Molecular and Cellular Cardiology, Vol. 46, Issue 3 https://doi.org/10.1016/j.yjmcc.2008.10.024	journal	March 2009
A Fluorophore-Doped Polymer Nanomaterial for Referenced Imaging of pH and Temperature with Sub-Micrometer Resolution Wang, Xu-dong; Meier, Robert J.; Wolfbeis, Otto S. Advanced Functional Materials, Vol. 22, Issue 20 https://doi.org/10.1002/adfm.201200813	journal	June 2012
Ultrasensitive, Biocompatible, Self-Calibrating, Multiparametric Temperature Sensors Zhao, Haiguang; Vomiero, Alberto; Rosei, Federico Small, Vol. 11, Issue 43 https://doi.org/10.1002/smll.201502249	journal	October 2015
Spectroscopic and Device Aspects of Nanocrystal Quantum Dots Pietryga, Jeffrey M.; Park, Young-Shin; Lim, Jaehoon Chemical Reviews, Vol. 116, Issue 18 https://doi.org/10.1021/acs.chemrev.6b00169	journal	September 2016
Stiffness-Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles Hartmann, Raimo; Weidenbach, Marcel; Neubauer, Martin Angewandte Chemie International Edition, Vol. 54, Issue 4 https://doi.org/10.1002/anie.201409693	journal	December 2014
Electrochemical Control of Two-Color Emission from Colloidal Dot-in-Bulk Nanocrystals Brovelli, Sergio; Bae, Wan Ki; Meinardi, Francesco Nano Letters, Vol. 14, Issue 7 https://doi.org/10.1021/nl501026r	journal	June 2014
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics Michalet, X. Science, Vol. 307, Issue 5709 https://doi.org/10.1126/science.1104274	journal	January 2005
Fluorescent probes and nanoparticles for intracellular sensing of pH values Shi, Wen; Li, Xiaohua; Ma, Huimin Methods and Applications in Fluorescence, Vol. 2, Issue 4 https://doi.org/10.1088/2050-6120/2/4/042001	journal	December 2014
Near Infrared Fluorescence-Based Bacteriophage Particles for Ratiometric pH Imaging Hilderbrand, Scott A.; Kelly, Kimberly A.; Niedre, Mark Bioconjugate Chemistry, Vol. 19, Issue 8 https://doi.org/10.1021/bc800188p	journal	August 2008
Energy transduction in the F1 motor of ATP synthase Wang, Hongyun; Oster, George Nature, Vol. 396, Issue 6708 https://doi.org/10.1038/24409	journal	November 1998
Impact of fixation on in vitro cell culture lines monitored with Raman spectroscopy Mariani, Melissa M.; Lampen, Peter; Popp, Jürgen The Analyst, Vol. 134, Issue 6 https://doi.org/10.1039/b822408k	journal	January 2009
Alterations of intracellular pH homeostasis in apoptosis: origins and roles Lagadic-Gossmann, D.; Huc, L.; Lecureur, V. Cell Death & Differentiation, Vol. 11, Issue 9 https://doi.org/10.1038/sj.cdd.4401466	journal	June 2004
A quantum dot-based ratiometric pH sensor Jin, Takashi; Sasaki, Akira; Kinjo, Masataka Chemical Communications, Vol. 46, Issue 14 https://doi.org/10.1039/b921602b	journal	January 2010
Size tuning of MAA capped CdSe and CdSe/CdS quantum dots and their stability in different pH environments Kaur, Gurvir; Tripathi, S. K. Materials Chemistry and Physics, Vol. 143, Issue 2 https://doi.org/10.1016/j.matchemphys.2013.09.003	journal	January 2014
Quantum dot bioconjugates for imaging, labelling and sensing Medintz, Igor L.; Uyeda, H. Tetsuo; Goldman, Ellen R. Nature Materials, Vol. 4, Issue 6, p. 435-446 https://doi.org/10.1038/nmat1390	journal	June 2005
Fluorescence ratiometric pH sensor prepared from covalently immobilized porphyrin and benzothioxanthene Niu, Cheng-Gang; Gui, Xiao-Qin; Zeng, Guang-Ming Analytical and Bioanalytical Chemistry, Vol. 383, Issue 2 https://doi.org/10.1007/s00216-005-3422-y	journal	August 2005
Coupled and Decoupled Dual Quantum Systems in One Semiconductor Nanocrystal Battaglia, David; Blackman, Bridgette; Peng, Xiaogang Journal of the American Chemical Society, Vol. 127, Issue 31 https://doi.org/10.1021/ja0437297	journal	August 2005
A hybrid bioorganic interface for neuronal photoactivation Ghezzi, Diego; Antognazza, Maria Rosa; Dal Maschio, Marco Nature Communications, Vol. 2, Issue 1 https://doi.org/10.1038/ncomms1164	journal	January 2011
A novel pH “off–on” fluorescent probe for lysosome imaging Chen, Laizhong; Li, Jing; Liu, Zhenzhen RSC Advances, Vol. 3, Issue 32 https://doi.org/10.1039/c3ra41898g	journal	January 2013
Core-shell type dually fluorescent polymer nanoparticles for ratiometric pH-sensing Allard, Emmanuel; Larpent, Chantal Journal of Polymer Science Part A: Polymer Chemistry, Vol. 46, Issue 18 https://doi.org/10.1002/pola.22931	journal	September 2008
On the pH-Dependent Quenching of Quantum Dot Photoluminescence by Redox Active Dopamine Ji, Xin; Palui, Goutam; Avellini, Tommaso Journal of the American Chemical Society, Vol. 134, Issue 13 https://doi.org/10.1021/ja300724x	journal	March 2012
The surface science of nanocrystals Boles, Michael A.; Ling, Daishun; Hyeon, Taeghwan Nature Materials, Vol. 15, Issue 2 https://doi.org/10.1038/nmat4526	journal	January 2016
A Tunable Ratiometric pH Sensor Based on Carbon Nanodots for the Quantitative Measurement of the Intracellular pH of Whole Cells Shi, Wen; Li, Xiaohua; Ma, Huimin Angewandte Chemie International Edition, Vol. 51, Issue 26 https://doi.org/10.1002/anie.201202533	journal	May 2012
Subcellular optochemical nanobiosensors: probes encapsulated by biologically localised embedding (PEBBLEs) Clark, Heather A.; Barker, Susan L. R.; Brasuel, Murphy Sensors and Actuators B: Chemical, Vol. 51, Issue 1-3 https://doi.org/10.1016/S0925-4005(98)00212-3	journal	August 1998
Spectral imaging microscopy demonstrates cytoplasmic pH oscillations in glial cells Sánchez-Armáss, Sergio; Sennoune, Souad R.; Maiti, Debasish American Journal of Physiology-Cell Physiology, Vol. 290, Issue 2 https://doi.org/10.1152/ajpcell.00290.2005	journal	February 2006
A polymer optoelectronic interface restores light sensitivity in blind rat retinas Ghezzi, Diego; Antognazza, Maria Rosa; Maccarone, Rita Nature Photonics, Vol. 7, Issue 5 https://doi.org/10.1038/nphoton.2013.34	journal	March 2013
Use of MTT colorimetric assay to measure cell activation Gerlier, Denis; Thomasset, Nicole Journal of Immunological Methods, Vol. 94, Issue 1-2 https://doi.org/10.1016/0022-1759(86)90215-2	journal	November 1986
Dynamic Hole Blockade Yields Two-Color Quantum and Classical Light from Dot-in-Bulk Nanocrystals Galland, Christophe; Brovelli, Sergio; Bae, Wan Ki Nano Letters, Vol. 13, Issue 1 https://doi.org/10.1021/nl3045316	journal	December 2012
Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding Gao, Xiaohu; Chan, Warren C. W.; Nie, Shuming Journal of Biomedical Optics, Vol. 7, Issue 4 https://doi.org/10.1117/1.1506706	journal	January 2002
Comparison of three cell fixation methods for high content analysis assays utilizing quantum dots Williams, Y.; Byrne, S.; Bashir, M. Journal of Microscopy, Vol. 232, Issue 1 https://doi.org/10.1111/j.1365-2818.2008.02083.x	journal	October 2008
Ratiometric pH-dot ANSors Wang, Xiaojuan; Boschetti, Chiara; Ruedas-Rama, Maria Jose The Analyst, Vol. 135, Issue 7 https://doi.org/10.1039/b922751b	journal	January 2010
pH-Sensitive Capsules as Intracellular Optical Reporters for Monitoring Lysosomal pH Changes Upon Stimulation RiveraGil, Pilar; Nazarenus, Moritz; Ashraf, Sumaira Small, Vol. 8, Issue 6 https://doi.org/10.1002/smll.201101780	journal	February 2012
Full-Range Intracellular pH Sensing by an Aggregation-Induced Emission-Active Two-Channel Ratiometric Fluorogen Chen, Sijie; Hong, Yuning; Liu, Yang Journal of the American Chemical Society, Vol. 135, Issue 13 https://doi.org/10.1021/ja400337p	journal	March 2013
Sensitive and Selective Near-Infrared Fluorescent Off–On Probe and Its Application to Imaging Different Levels of β-Lactamase in Staphylococcus aureus Li, Lihong; Li, Zhao; Shi, Wen Analytical Chemistry, Vol. 86, Issue 12 https://doi.org/10.1021/ac501288e	journal	May 2014
The effects of basic substances and acidic ionophores on the digestion of exogenous and endogenous proteins in mouse peritoneal macrophages Ohkuma, S. The Journal of Cell Biology, Vol. 102, Issue 3 https://doi.org/10.1083/jcb.102.3.959	journal	March 1986
Dual-color emitting quantum-dot-quantum-well CdSe-ZnS heteronanocrystals hybridized on InGaN∕GaN light emitting diodes for high-quality white light generation Nizamoglu, Sedat; Mutlugun, Evren; Özel, Tuncay Applied Physics Letters, Vol. 92, Issue 11 https://doi.org/10.1063/1.2898892	journal	March 2008
Fluorescent core–shell silica nanoparticles: towards “Lab on a Particle” architectures for nanobiotechnology Burns, Andrew; Ow, Hooisweng; Wiesner, Ulrich Chem. Soc. Rev., Vol. 35, Issue 11, p. 1028-1042 https://doi.org/10.1039/B600562B	journal	September 2006
Histidine button engineered into cardiac troponin I protects the ischemic and failing heart Day, Sharlene M.; Westfall, Margaret V.; Fomicheva, Ekaterina V. Nature Medicine, Vol. 12, Issue 2 https://doi.org/10.1038/nm1346	journal	January 2006
Are cancer cells acidic? Griffiths, Jr British Journal of Cancer, Vol. 64, Issue 3 https://doi.org/10.1038/bjc.1991.326	journal	September 1991
Quantum Dot FRET Biosensors that Respond to pH, to Proteolytic or Nucleolytic Cleavage, to DNA Synthesis, or to a Multiplexing Combination Suzuki, Miho; Husimi, Yuzuru; Komatsu, Hirokazu Journal of the American Chemical Society, Vol. 130, Issue 17 https://doi.org/10.1021/ja710870e	journal	April 2008
A Ratiometric CdSe/ZnS Nanocrystal pH Sensor Snee, Preston T.; Somers, Rebecca C.; Nair, Gautham Journal of the American Chemical Society, Vol. 128, Issue 41 https://doi.org/10.1021/ja0618999	journal	October 2006
Studies of chemical fixation effects in human cell lines using Raman microspectroscopy Meade, Aidan D.; Clarke, Colin; Draux, Florence Analytical and Bioanalytical Chemistry, Vol. 396, Issue 5 https://doi.org/10.1007/s00216-009-3411-7	journal	January 2010
Synthesis of CdSe and CdTe Nanocrystals without Precursor Injection Yang, Yongan Andrew; Wu, Huimeng; Williams, Kathryn R. Angewandte Chemie International Edition, Vol. 44, Issue 41 https://doi.org/10.1002/anie.200502279	journal	October 2005
Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing Medintz, Igor L.; Stewart, Michael H.; Trammell, Scott A. Nature Materials, Vol. 9, Issue 8 https://doi.org/10.1038/nmat2811	journal	August 2010
Formaldehyde fixation. Fox, C. H.; Johnson, F. B.; Whiting, J. Journal of Histochemistry & Cytochemistry, Vol. 33, Issue 8 https://doi.org/10.1177/33.8.3894502	journal	August 1985
A Near-Infrared Neutral pH Fluorescent Probe for Monitoring Minor pH Changes: Imaging in Living HepG2 and HL-7702 Cells Tang, Bo; Yu, Fabiao; Li, Ping Journal of the American Chemical Society, Vol. 131, Issue 8 https://doi.org/10.1021/ja809149g	journal	March 2009
Increased pH and tumorigenicity of fibroblasts expressing a yeast proton pump Perona, Rosario; Serrano, Ramon Nature, Vol. 334, Issue 6181 https://doi.org/10.1038/334438a0	journal	August 1988
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays Mosmann, Tim Journal of Immunological Methods, Vol. 65, Issue 1-2 https://doi.org/10.1016/0022-1759(83)90303-4	journal	December 1983
pH-Sensitive Quantum Dots Tomasulo, Massimiliano; Yildiz, Ibrahim; Raymo, Françisco M. The Journal of Physical Chemistry B, Vol. 110, Issue 9 https://doi.org/10.1021/jp060185h	journal	March 2006
pH-Sensitive Photoluminescence of CdSe/ZnSe/ZnS Quantum Dots in Human Ovarian Cancer Cells Liu, Yu-San; Sun, Yinghua; Vernier, P. Thomas The Journal of Physical Chemistry C, Vol. 111, Issue 7 https://doi.org/10.1021/jp0654718	journal	January 2007
Functionalized Metallic Carbon Nanotube Devices for pH Sensing Maroto, Alicia; Balasubramanian, Kannan; Burghard, Marko ChemPhysChem, Vol. 8, Issue 2 https://doi.org/10.1002/cphc.200600498	journal	February 2007
A Nanogel for Ratiometric Fluorescent Sensing of Intracellular pH Values Peng, Hong-shang; Stolwijk, Judith A.; Sun, Li-Ning Angewandte Chemie International Edition, Vol. 49, Issue 25 https://doi.org/10.1002/anie.200906926	journal	May 2010
Simultaneous Type-I/Type-II Emission from CdSe/CdS/ZnSe Nano-Heterostructures Soni, Udit; Pal, Anuushka; Singh, Sajan ACS Nano, Vol. 8, Issue 1 https://doi.org/10.1021/nn404537s	journal	December 2013
Localization and Dynamics of Long-Lived Excitations in Colloidal Semiconductor Nanocrystals with Dual Quantum Confinement Liu, Su; Borys, Nicholas J.; Sapra, Sameer ChemPhysChem, Vol. 16, Issue 8 https://doi.org/10.1002/cphc.201402826	journal	March 2015
One-pot synthesis of fluorescent BSA–Ce/Au nanoclusters as ratiometric pH probes Chen, Ya-Na; Chen, Po-Cheng; Wang, Chia-Wei Chemical Communications, Vol. 50, Issue 62 https://doi.org/10.1039/C4CC03949A	journal	January 2014
Design and Synthesis of Heterostructured Quantum Dots with Dual Emission in the Visible and Infrared Lin, Qianglu; Makarov, Nikolay S.; Koh, Weon-kyu ACS Nano, Vol. 9, Issue 1 https://doi.org/10.1021/nn505793y	journal	December 2014
Surface Polarization Drives Photoinduced Charge Separation at the P3HT/Water Interface Mosconi, Edoardo; Salvatori, Paolo; Saba, Maria Ilenia ACS Energy Letters, Vol. 1, Issue 2 https://doi.org/10.1021/acsenergylett.6b00197	journal	August 2016
Sensors and regulators of intracellular pH Casey, Joseph R.; Grinstein, Sergio; Orlowski, John Nature Reviews Molecular Cell Biology, Vol. 11, Issue 1 https://doi.org/10.1038/nrm2820	journal	December 2009
Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins Miesenböck, Gero; De Angelis, Dino A.; Rothman, James E. Nature, Vol. 394, Issue 6689 https://doi.org/10.1038/28190	journal	July 1998
Immunolabeling artifacts and the need for live-cell imaging Schnell, Ulrike; Dijk, Freark; Sjollema, Klaas A. Nature Methods, Vol. 9, Issue 2 https://doi.org/10.1038/nmeth.1855	journal	January 2012
A series of naphthalimide derivatives as intra and extracellular pH sensors Tian, Yanqing; Su, Fengyu; Weber, Warner Biomaterials, Vol. 31, Issue 29 https://doi.org/10.1016/j.biomaterials.2010.06.023	journal	October 2010
Metabolic regulation via intracellular pH Busa, W. B.; Nuccitelli, R. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 246, Issue 4 https://doi.org/10.1152/ajpregu.1984.246.4.R409	journal	April 1984
The role of pH dynamics and the Na+/H+ antiporter in the etiopathogenesis and treatment of cancer. Two faces of the same coin—one single nature Harguindey, Salvador; Orive, Gorka; Luis Pedraz, José Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Vol. 1756, Issue 1 https://doi.org/10.1016/j.bbcan.2005.06.004	journal	September 2005

Cited By (4)

Highly Stable Core–Shell Structured Semiconducting Polymer Nanoparticles for FRET‐Based Intracellular pH Imaging Bao, Biqing; Su, Peng; Yang, Zhenyuan Advanced Healthcare Materials, Vol. 8, Issue 14 https://doi.org/10.1002/adhm.201900255	journal	May 2019
Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix Clasen, Anne; Wenderoth, Sarah; Tavernaro, Isabella RSC Advances, Vol. 9, Issue 61 https://doi.org/10.1039/c9ra06047b	journal	January 2019
Quantum-confined stark effect in the ensemble of phase-pure CdSe/CdS quantum dots Zhang, Lei; Lv, Bihu; Yang, Hongyu Nanoscale, Vol. 11, Issue 26 https://doi.org/10.1039/c9nr03061a	journal	January 2019
Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells Bossio, Caterina; Abdel Aziz, Ilaria; Tullii, Gabriele Frontiers in Bioengineering and Biotechnology, Vol. 6 https://doi.org/10.3389/fbioe.2018.00114	journal	August 2018

Similar Records

Enhancement of T1 and T2 relaxation by paramagnetic silica-coated nanocrystals

Journal Article · Mon Aug 28 00:00:00 EDT 2006 · Journal of Physical Chemistry C, vol. 111, n/a, August 1, 2007, pp. 540-551 · OSTI ID:1481142

Gerion, D; Herberg, J; Gjersing, E; +5 more

Fluorescent protein Dendra2 as a ratiometric genetically encoded pH-sensor

Journal Article · Sat Dec 02 00:00:00 EST 2017 · Biochemical and Biophysical Research Communications · OSTI ID:1481142

Martynov, Vladimir I.; Orsa, Alexander N.; Bondarenko, Alena A.; +4 more

An accessible approach to preparing water-soluble Mn²⁺-doped (CdSSe)ZnS (core)shell nanocrystals for ratiometric temperature sensing

Journal Article · Fri Oct 28 00:00:00 EDT 2011 · ACS Nano · OSTI ID:1481142

Hsia, Chih -Hao; Wuttig, Anna; Yang, Haw

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
36 MATERIALS SCIENCE
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
bioimaging
colloidal quantum dots
pH sensing
ratiometric sensing
two‐color emission

Title: Two-Color Emitting Colloidal Nanocrystals as Single-Particle Ratiometric Probes of Intracellular pH

Citation Formats

References (81)

Cited By (4)

Similar Records

Related Subjects