Rapid sample delivery for megahertz serial crystallography at X-ray FELs

Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.; Ayyer, Kartik; Gevorkov, Yaroslav; Fleckenstein, Holger; Roth, Nils; Adriano, Luigi; Bean, Richard; Beyerlein, Kenneth R.; Chen, Joe; Coe, Jesse; Cruz-Mazo, Francisco; Ekeberg, Tomas; Graceffa, Rita; Heymann, Michael; Horke, Daniel A.; Knoška, Juraj; Mariani, Valerio; Nazari, Reza; Oberthür, Dominik; Samanta, Amit K.; Sierra, Raymond G.; Stan, Claudiu A.; Yefanov, Oleksandr; Rompotis, Dimitrios; Correa, Jonathan; Erk, Benjamin; Treusch, Rolf; Schulz, Joachim; Hogue, Brenda G.; Gañán-Calvo, Alfonso M.; Fromme, Petra; Küpper, Jochen; Rode, Andrei V.; Bajt, Saša; Kirian, Richard A.; Chapman, Henry N.

doi:10.1107/s2052252518008369

Title: Rapid sample delivery for megahertz serial crystallography at X-ray FELs

Journal Article · Fri Jul 27 00:00:00 EDT 2018 · IUCrJ

DOI:https://doi.org/10.1107/s2052252518008369· OSTI ID:1475541

^[1]; Awel, Salah ^[2]; Morgan, Andrew J. ^[3]; Ayyer, Kartik ^[3]; Gevorkov, Yaroslav ^[4]; Fleckenstein, Holger ^[3]; Roth, Nils ^[5]; Adriano, Luigi ^[6]; Bean, Richard ^[7]; Beyerlein, Kenneth R. ^[3]; Chen, Joe ^[8]; Coe, Jesse ^[8]; Cruz-Mazo, Francisco ^[9]; Ekeberg, Tomas ^[3]; Graceffa, Rita ^[7]; Heymann, Michael ^[10]; Horke, Daniel A. ^[2]; Knoška, Juraj ^[5]; Mariani, Valerio ^[3]; Nazari, Reza ^[8] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Hamburg Univ. of Technology, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
Arizona State Univ., Tempe, AZ (United States)
Univ. de Sevilla, Sevilla (Spain). Dept. of Aerospace Engineering and Fluid Mechanics
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Max Planck Inst. of Biochemistry, Martinsried (Germany). Dept. of Cellular and Molecular Biophysics
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Rutgers University Newark, Newark, NJ (United States). Dept. of Physics; SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science, and Photon Science
Arizona State Univ., Mesa, AZ (United States). Biodesign Inst., School of Life Sciences
Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., School of Life Sciences
Australian National Univ., Canberra, ACT (Australia). Research School of Physical Sciences and Engineering, Laser Physics Centre

Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. The high X-ray intensity in the focus initiates an explosion of the microjet and sample. With the advent of X-ray FELs with megahertz rates, the typical velocities of these jets must be increased significantly in order to replenish the damaged material in time for the subsequent measurement with the next X-ray pulse. Here in this paper, this work reports the results of a megahertz serial diffraction experiment at the FLASH FEL facility using 4.3 nm radiation. The operation of gas-dynamic nozzles that produce liquid microjets with velocities greater than 80 m s^-1was demonstrated. Furthermore, this article provides optical images of X-ray-induced explosions together with Bragg diffraction from protein microcrystals exposed to trains of X-ray pulses repeating at rates of up to 4.5 MHz. Finally, the results indicate the feasibility for megahertz serial crystallography measurements with hard X-rays and give guidance for the design of such experiments.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE; European Union (EU); Australian Research Council (ARC); German Research Foundation (DFG)

Grant/Contract Number:: AC02-76SF00515; DPI2016-78887; ERC-614507-Kupper

OSTI ID:: 1475541

Journal Information:: IUCrJ, Vol. 5, Issue 5; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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On the characterization of a 1 m long, ultra-precise KB-focusing mirror pair for European XFEL by means of slope measuring deflectometry Siewert, F.; Buchheim, J.; Gwalt, G. Review of Scientific Instruments, Vol. 90, Issue 2 https://doi.org/10.1063/1.5065473	journal	February 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains Yefanov, Oleksandr; Oberthür, Dominik; Bean, Richard Structural Dynamics, Vol. 6, Issue 6 https://doi.org/10.1063/1.5124387	journal	November 2019
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Megahertz serial crystallography Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-03705	text	January 2018
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Evaluation of serial crystallographic structure determination within megahertz pulse trains Yefanov, Oleksandr; Oberthür, Dominik; Bean, Richard Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-04763	text	January 2019
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00687	text	January 2020
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X-ray free-electron lasers
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Title: Rapid sample delivery for megahertz serial crystallography at X-ray FELs

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