Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum

Xiao, Rui; Yang, Xi; Li, Mi; Li, Xiang; Wei, Yanzhang; Cao, Min; Ragauskas, Arthur; Thies, Mark; Ding, Junhuan; Zheng, Yi

doi:10.1016/j.carbpol.2018.04.126

Title: Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum

Journal Article · Tue May 01 00:00:00 EDT 2018 · Carbohydrate Polymers

DOI:https://doi.org/10.1016/j.carbpol.2018.04.126· OSTI ID:1474532

Xiao, Rui ^[1]; Yang, Xi ^[1]; Li, Mi ^[2]; Li, Xiang ^[1]; Wei, Yanzhang ^[1]; Cao, Min ^[1];

^[3]; Thies, Mark ^[1]; Ding, Junhuan ^[1];

^[1]

Clemson Univ., SC (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities. Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared. The EPSs from both strains mainly contained polysaccharide (41–64%, w/w, dry basis) and protein (25–40%, w/w, dry basis), which was shown by the morphology study with an AFM. The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose. Glucose and arabinose took up to 82–90% (w/w, dry basis) of the total polysaccharide. The structure and functional groups of EPSs were determined by FTIR and NMR. The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose. The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549. The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1474532

Journal Information:: Carbohydrate Polymers, Vol. 195, Issue C; ISSN 0144-8617

Publisher:: Applied Science DirectCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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