Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors

Ruckh, Timothy T.; Skipwith, Christopher G.; Chang, Wendi; Senko, Alexander W.; Bulovic, Vladimir; Anikeeva, Polina O.; Clark, Heather A.

doi:10.1021/acsnano.5b05396

Title: Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors

Journal Article · Mon Apr 18 00:00:00 EDT 2016 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.5b05396· OSTI ID:1467074

Ruckh, Timothy T. ^[1]; Skipwith, Christopher G. ^[1]; Chang, Wendi ^[2]; Senko, Alexander W. ^[3]; Bulovic, Vladimir ^[2]; Anikeeva, Polina O. ^[3]; Clark, Heather A. ^[1]

Northeastern Univ., Boston, MA (United States). Dept. of Pharmaceutical Sciences
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Electrical Engineering
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Materials Science and Engineering

The tools for optically imaging cellular potassium concentrations in real-time are currently limited to a small set of molecular indicator dyes. Quantum dot-based nanosensors are more photostable and tunable than organic indicators, but previous designs have fallen short in size, sensitivity, and selectivity. In this paper, we introduce a small, sensitive, and selective nanosensor for potassium measurements. A dynamic quencher modulates the fluorescence emitted by two different quantum dot species to produce a ratiometric signal. We characterized the potassium-modulated sensor properties and investigated the photonic interactions within the sensors. The quencher’s protonation changes in response to potassium, which modulates its Förster radiative energy transfer rate and the corresponding interaction radii with each quantum dot species. Finally, the nanosensors respond to changes in potassium concentrations typical of the cellular environment and thus provide a promising tool for imaging potassium fluxes during biological events.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Northeastern Univ., Boston, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States); National Academy of Sciences Ford Foundation (United States)

Grant/Contract Number:: FG02-07ER46474; R01NS081641; F32EB015847; 2014-PGA084488

OSTI ID:: 1467074

Journal Information:: ACS Nano, Vol. 10, Issue 4; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 44 works

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An ionophore-based persistent luminescent ‘Glow Sensor’ for sodium detection Ferris, Mark S.; Behr, Madeline R.; Cash, Kevin J. RSC Advances, Vol. 9, Issue 56 https://doi.org/10.1039/c9ra05313a	journal	January 2019
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Title: Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors

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