Safeguarding CRISPR-Cas9 gene drives in yeast

DiCarlo, James E.; Chavez, Alejandro; Dietz, Sven L.; Esvelt, Kevin M.; Church, George M.

doi:10.1038/nbt.3412

Title: Safeguarding CRISPR-Cas9 gene drives in yeast

Journal Article · Mon Nov 16 00:00:00 EST 2015 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.3412· OSTI ID:1466972

DiCarlo, James E. ^[1]; Chavez, Alejandro ^[2]; Dietz, Sven L. ^[3];

^[4]; Church, George M. ^[5]

Harvard Medical School, Boston, MA (United States). Dept. of Genetics; Boston Univ., MA (United States). Dept. of Biomedical Engineering
Harvard Medical School, Boston, MA (United States). Dept. of Genetics; Wyss Inst. for Biologically Inspired Engineering, Boston, MA (United States); Massachusetts General Hospital, Boston, MA (United States). Dept. of Pathology
Harvard Medical School, Boston, MA (United States). Dept. of Genetics; Wyss Inst. for Biologically Inspired Engineering, Boston, MA (United States); ETH Zurich, Basel (Switzerland). Dept. of Biosystems Science and Engineering
Harvard Medical School, Boston, MA (United States); Wyss Inst. for Biologically Inspired Engineering, Boston, MA (United States)
Harvard Medical School, Boston, MA (United States). Dept. of Genetics; Wyss Inst. for Biologically Inspired Engineering, Boston, MA (United States)

RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the prospect of unanticipated ecological change, demands caution. In this, we report the efficacy of CRISPR-Cas9 gene drive systems in wild and laboratory strains of the yeast Saccharomyces cerevisiae. Furthermore, we address concerns surrounding accidental genome editing by developing and validating methods of molecular confinement that minimize the risk of unwanted genome editing. We also present a drive system capable of overwriting the changes introduced by an earlier gene drive. These molecular safeguards should enable the development of safe CRISPR gene drives for diverse organisms.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: FG02-02ER63445; SynBERC SA5283-11210; MCB-1330914; 5T32CA009216-34; 1K99DK102669-01

OSTI ID:: 1466972

Journal Information:: Nature Biotechnology, Vol. 33, Issue 12; ISSN 1087-0156

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 195 works

Citation information provided by
Web of Science

References (36)

Monitoring Select Agent Theft, Loss and Release Reports in the United States—2004–2010 Henkel, Richard D.; Miller, Thomas; Weyant, Robbin S. Applied Biosafety, Vol. 17, Issue 4 https://doi.org/10.1177/153567601201700402	journal	December 2012
Break-Induced Replication Repair of Damaged Forks Induces Genomic Duplications in Human Cells Costantino, Lorenzo; Sotiriou, Sotirios K.; Rantala, Juha K. Science, Vol. 343, Issue 6166 https://doi.org/10.1126/science.1243211	journal	December 2013
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease Gratz, Scott J.; Cummings, Alexander M.; Nguyen, Jennifer N. Genetics, Vol. 194, Issue 4 https://doi.org/10.1534/genetics.113.152710	journal	May 2013
Optimising Homing Endonuclease Gene Drive Performance in a Semi-Refractory Species: The Drosophila melanogaster Experience Chan, Yuk-Sang; Huen, David S.; Glauert, Ruth PLoS ONE, Vol. 8, Issue 1 https://doi.org/10.1371/journal.pone.0054130	journal	January 2013
Heritable genome editing in C. elegans via a CRISPR-Cas9 system Friedland, Ari E.; Tzur, Yonatan B.; Esvelt, Kevin M. Nature Methods, Vol. 10, Issue 8 https://doi.org/10.1038/nmeth.2532	journal	June 2013
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems DiCarlo, James E.; Norville, Julie E.; Mali, Prashant Nucleic Acids Research, Vol. 41, Issue 7, p. 4336-4343 https://doi.org/10.1093/nar/gkt135	journal	March 2013
The Formation of Pigment and Arylamine by Yeasts Chamberlain, N.; Cutts, N. S.; Rainbow, C. Journal of General Microbiology, Vol. 7, Issue 1-2 https://doi.org/10.1099/00221287-7-1-2-54	journal	August 1952
A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish Ablain, Julien; Durand, Ellen M.; Yang, Song Developmental Cell, Vol. 32, Issue 6 https://doi.org/10.1016/j.devcel.2015.01.032	journal	March 2015
BoxPlotR: a web tool for generation of box plots Spitzer, Michaela; Wildenhain, Jan; Rappsilber, Juri Nature Methods, Vol. 11, Issue 2 https://doi.org/10.1038/nmeth.2811	journal	January 2014
Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization Takeuchi, R.; Choi, M.; Stoddard, B. L. Proceedings of the National Academy of Sciences, Vol. 111, Issue 11 https://doi.org/10.1073/pnas.1321030111	journal	March 2014
Homing endonuclease genes: the rise and fall and rise again of a selfish element Burt, Austin; Koufopanou, Vassiliki Current Opinion in Genetics & Development, Vol. 14, Issue 6 https://doi.org/10.1016/j.gde.2004.09.010	journal	December 2004
Safeguarding gene drive experiments in the laboratory Akbari, O. S.; Bellen, H. J.; Bier, E. Science, Vol. 349, Issue 6251 https://doi.org/10.1126/science.aac7932	journal	July 2015
A synthetic homing endonuclease-based gene drive system in the human malaria mosquito Windbichler, Nikolai; Menichelli, Miriam; Papathanos, Philippos Aris Nature, Vol. 473, Issue 7346 https://doi.org/10.1038/nature09937	journal	April 2011
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering Wang, Haoyi; Yang, Hui; Shivalila, Chikdu S. Cell, Vol. 153, Issue 4, p. 910-918 https://doi.org/10.1016/j.cell.2013.04.025	journal	May 2013
The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations Gantz, V. M.; Bier, E. Science, Vol. 348, Issue 6233 https://doi.org/10.1126/science.aaa5945	journal	March 2015
A recombination execution checkpoint regulates the choice of homologous recombination pathway during DNA double-strand break repair Jain, S.; Sugawara, N.; Lydeard, J. Genes & Development, Vol. 23, Issue 3 https://doi.org/10.1101/gad.1751209	journal	February 2009
Site-specific selfish genes as tools for the control and genetic engineering of natural populations Burt, Austin Proceedings of the Royal Society of London. Series B: Biological Sciences, Vol. 270, Issue 1518 https://doi.org/10.1098/rspb.2002.2319	journal	May 2003
RNA-Guided Human Genome Engineering via Cas9 Mali, P.; Yang, L.; Esvelt, K. M. Science, Vol. 339, Issue 6121, p. 823-826 https://doi.org/10.1126/science.1232033	journal	January 2013
Multiplex Genome Engineering Using CRISPR/Cas Systems Cong, L.; Ran, F. A.; Cox, D. Science, Vol. 339, Issue 6121, p. 819-823 https://doi.org/10.1126/science.1231143	journal	January 2013
Regulating gene drives Oye, K. A.; Esvelt, K.; Appleton, E. Science, Vol. 345, Issue 6197 https://doi.org/10.1126/science.1254287	journal	July 2014
RNA-programmed genome editing in human cells Jinek, Martin; East, Alexandra; Cheng, Aaron eLife, Vol. 2, Article No. e00471 https://doi.org/10.7554/eLife.00471	journal	January 2013
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice Jiang, Wenzhi; Zhou, Huanbin; Bi, Honghao Nucleic Acids Research, Vol. 41, Issue 20 https://doi.org/10.1093/nar/gkt780	journal	August 2013
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila Port, F.; Chen, H. -M.; Lee, T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 29 https://doi.org/10.1073/pnas.1405500111	journal	July 2014
Concerning RNA-Guided Gene Drives for the Alteration of Wild Populations Esvelt, Kevin M.; Smidler, Andrea L.; Catteruccia, Flaminia eLife https://doi.org/10.1101/007203	journal	July 2014
Extraction of genomic DNA from yeasts for PCR-based applications Lõoke, Marko; Kristjuhan, Kersti; Kristjuhan, Arnold BioTechniques, Vol. 50, Issue 5 https://doi.org/10.2144/000113672	journal	May 2011
Entamoeba histolytica exploits the autophagy pathway in macrophages to trigger inflammation in disease pathogenesis Begum, Sharmin; Moreau, France; Dufour, Antoine Mucosal Immunology, Vol. 14, Issue 5 https://doi.org/10.1038/s41385-021-00408-4	journal	May 2021
CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions Kaushal, Anjali; Mohana, Giriram; Dorier, Julien Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21366-2	journal	February 2021
Optimising homing endonuclease gene drive performance in a semi-refractory species: the Drosophila melanogaster experience. Chan, Yuk-Sang; Huen, David S.; Glauert, Ruth Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.21581	text	January 2013
Concerning RNA-guided gene drives for the alteration of wild populations Esvelt, Kevin M.; Smidler, Andrea L.; Catteruccia, Flaminia eLife, Vol. 3 https://doi.org/10.7554/eLife.03401	journal	July 2014
RNA-programmed genome editing in human cells Jinek, Martin; East, Alexandra; Cheng, Aaron eLife Sciences Publications Ltd. https://doi.org/10.5167/uzh-79770	text	January 2013
Transposition of an intron in yeast mitochondria requires a protein encoded by that intron Macreadie, I.; Scott, R.; Zinn, A. Cell, Vol. 41, Issue 2 https://doi.org/10.1016/s0092-8674(85)80012-x	journal	June 1985
THE ENTIRE COMPOUND AUTOSOMES OF DROSOPHILA MELANOGASTER Novitski, E.; Grace, D.; Strommen, C. Genetics, Vol. 98, Issue 2 https://doi.org/10.1093/genetics/98.2.257	journal	June 1981
Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: cap methyltransferase activity is essential for cell growth. Mao, X.; Schwer, B.; Shuman, S. Molecular and Cellular Biology, Vol. 16, Issue 2 https://doi.org/10.1128/mcb.16.2.475	journal	February 1996
Insect Population Control by Homing Endonuclease-Based Gene Drive: An Evaluation inDrosophila melanogaster Chan, Yuk-Sang; Naujoks, Daniel A.; Huen, David S. Genetics, Vol. 188, Issue 1 https://doi.org/10.1534/genetics.111.127506	journal	May 2011
Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila Kondo, Shu; Ueda, Ryu Genetics, Vol. 195, Issue 3 https://doi.org/10.1534/genetics.113.156737	journal	September 2013
Extraction of genomic DNA from yeasts for PCR-based applications Lõoke, Marko; Kristjuhan, Kersti; Kristjuhan, Arnold BioTechniques, Vol. 62, Issue 1 https://doi.org/10.2144/000114497	journal	January 2017

Cited By (101)

Gene drive systems: do they have a place in agricultural weed management?: Gene drive and weed management Neve, Paul Pest Management Science, Vol. 74, Issue 12 https://doi.org/10.1002/ps.5137	journal	September 2018
Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system Zhao, Santao; Xing, Zengzhu; Liu, Zhonggeng Pest Management Science, Vol. 75, Issue 7 https://doi.org/10.1002/ps.5305	journal	January 2019
The emerging importance of noncoding RNAs in the insecticide tolerance, with special emphasis on Plutella xylostella (Lepidoptera: Plutellidae) Vaschetto, Luis María; Beccacece, Hernán Mario Wiley Interdisciplinary Reviews: RNA https://doi.org/10.1002/wrna.1539	journal	May 2019
Yeast genetic interaction screens in the age of CRISPR/Cas Adames, Neil R.; Gallegos, Jenna E.; Peccoud, Jean Current Genetics, Vol. 65, Issue 2 https://doi.org/10.1007/s00294-018-0887-8	journal	September 2018
Population management using gene drive: molecular design, models of spread dynamics and assessment of ecological risks Rode, Nicolas O.; Estoup, Arnaud; Bourguet, Denis Conservation Genetics, Vol. 20, Issue 4 https://doi.org/10.1007/s10592-019-01165-5	journal	April 2019
‘Gene drive’ moratorium shot down at UN biodiversity meeting Callaway, Ewen Nature https://doi.org/10.1038/nature.2016.21216	journal	December 2016
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae Hammond, Andrew; Galizi, Roberto; Kyrou, Kyros Nature Biotechnology, Vol. 34, Issue 1 https://doi.org/10.1038/nbt.3439	journal	January 2016
Cheating evolution: engineering gene drives to manipulate the fate of wild populations Champer, Jackson; Buchman, Anna; Akbari, Omar S. Nature Reviews Genetics, Vol. 17, Issue 3 https://doi.org/10.1038/nrg.2015.34	journal	February 2016
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast Buchmuller, Benjamin C.; Herbst, Konrad; Meurer, Matthias Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10816-7	journal	July 2019
A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus Valderrama, J. Andrés; Kulkarni, Surashree S.; Nizet, Victor Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-13649-6	journal	December 2019
A toxin-antidote CRISPR gene drive system for regional population modification Champer, Jackson; Lee, Esther; Yang, Emily Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14960-3	journal	February 2020
Next-generation biocontainment systems for engineered organisms Lee, Jeong Wook; Chan, Clement T. Y.; Slomovic, Shimyn Nature Chemical Biology, Vol. 14, Issue 6 https://doi.org/10.1038/s41589-018-0056-x	journal	May 2018
Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans Halder, Viola; Porter, Caroline B. M.; Chavez, Alejandro Nature Protocols, Vol. 14, Issue 3 https://doi.org/10.1038/s41596-018-0122-6	journal	February 2019
Development of a multi-locus CRISPR gene drive system in budding yeast Yan, Yao; Finnigan, Gregory C. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-34909-3	journal	November 2018
CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner Hanasaki, Miki; Masumoto, Hiroshi Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-51891-6	journal	October 2019
Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti Li, Ming; Bui, Michelle; Yang, Ting Proceedings of the National Academy of Sciences, Vol. 114, Issue 49 https://doi.org/10.1073/pnas.1711538114	journal	November 2017
Synthetically engineered Medea gene drive system in the worldwide crop pest Drosophila suzukii Buchman, Anna; Marshall, John M.; Ostrovski, Dennis Proceedings of the National Academy of Sciences, Vol. 115, Issue 18 https://doi.org/10.1073/pnas.1713139115	journal	April 2018
Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs Oberhofer, Georg; Ivy, Tobin; Hay, Bruce A. Proceedings of the National Academy of Sciences, Vol. 115, Issue 40 https://doi.org/10.1073/pnas.1805278115	journal	September 2018
Gene drives to fight malaria: current state and future directions Hammond, Andrew M.; Galizi, Roberto Pathogens and Global Health, Vol. 111, Issue 8 https://doi.org/10.1080/20477724.2018.1438880	journal	November 2017
Considerations for the governance of gene drive organisms Rudenko, Larisa; Palmer, Megan J.; Oye, Kenneth Pathogens and Global Health, Vol. 112, Issue 4 https://doi.org/10.1080/20477724.2018.1478776	journal	May 2018
Anticipating complexity in the deployment of gene drive insects in agriculture Baltzegar, Jennifer; Cavin Barnes, Jessica; Elsensohn, Johanna E. Journal of Responsible Innovation, Vol. 5, Issue sup1 https://doi.org/10.1080/23299460.2017.1407910	journal	December 2017
A roadmap for gene drives: using institutional analysis and development to frame research needs and governance in a systems context Kuzma, J.; Gould, F.; Brown, Z. Journal of Responsible Innovation, Vol. 5, Issue sup1 https://doi.org/10.1080/23299460.2017.1410344	journal	December 2017
Identifying and detecting potentially adverse ecological outcomes associated with the release of gene-drive modified organisms Hayes, Keith R.; Hosack, Geoffrey R.; Dana, Genya V. Journal of Responsible Innovation, Vol. 5, Issue sup1 https://doi.org/10.1080/23299460.2017.1415585	journal	January 2018
Threshold-Dependent Gene Drives in the Wild: Spread, Controllability, and Ecological Uncertainty Backus, Gregory A.; Delborne, Jason A. BioScience, Vol. 69, Issue 11 https://doi.org/10.1093/biosci/biz098	journal	September 2019
Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs Trasanidou, Despoina; Gerós, Ana Sousa; Mohanraju, Prarthana FEMS Microbiology Letters, Vol. 366, Issue 9 https://doi.org/10.1093/femsle/fnz098	journal	May 2019
A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae Reider Apel, Amanda; d'Espaux, Leo; Wehrs, Maren Nucleic Acids Research, Vol. 45, Issue 1 https://doi.org/10.1093/nar/gkw1023	journal	November 2016
Dodging silver bullets: good CRISPR gene-drive design is critical for eradicating exotic vertebrates Prowse, Thomas A. A.; Cassey, Phillip; Ross, Joshua V. Proceedings of the Royal Society B: Biological Sciences, Vol. 284, Issue 1860 https://doi.org/10.1098/rspb.2017.0799	journal	August 2017
Analysis of CRISPR gene drive design in budding yeast Yan, Yao; Finnigan, Gregory C. Access Microbiology, Vol. 1, Issue 9 https://doi.org/10.1099/acmi.0.000059	journal	November 2019
Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae Basgall, Erianna M.; Goetting, Samantha C.; Goeckel, Megan E. Microbiology, Vol. 164, Issue 4 https://doi.org/10.1099/mic.0.000635	journal	April 2018
Evolutionary dynamics of CRISPR gene drives Noble, Charleston; Olejarz, Jason; Esvelt, Kevin M. Science Advances https://doi.org/10.1101/057281	journal	June 2016
Novel CRISPR/Cas9 gene drive constructs in Drosophila reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations Champer, Jackson; Reeves, Riona; Yeon Oh, Suh PLOS Genetics https://doi.org/10.1101/112011	journal	February 2017
Performance analysis of novel toxin-antidote CRISPR gene drive systems Champer, Jackson; Kim, Isabel; Champer, Samuel E. BMC Biology https://doi.org/10.1101/628362	journal	May 2019
Split-gene drive system provides flexible application for safe laboratory investigation and potential field deployment Del Amo, Víctor López; Bishop, Alena L.; Sánchez C., Héctor M. Nature Communications https://doi.org/10.1101/684597	journal	June 2019
Viral gene drive in herpesviruses Walter, Marius; Verdin, Eric Nature Communications https://doi.org/10.1101/717017	journal	July 2019
Institutional Drift in International Biotechnology Regulation Rabitz, Florian Global Policy, Vol. 10, Issue 2 https://doi.org/10.1111/1758-5899.12652	journal	February 2019
Evolutionary dynamics of CRISPR gene drives Noble, Charleston; Olejarz, Jason; Esvelt, Kevin M. Science Advances, Vol. 3, Issue 4 https://doi.org/10.1126/sciadv.1601964	journal	April 2017
Does the U.S. public support using gene drives in agriculture? And what do they want to know? Jones, Michael S.; Delborne, Jason A.; Elsensohn, Johanna Science Advances, Vol. 5, Issue 9 https://doi.org/10.1126/sciadv.aau8462	journal	September 2019
Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs Champer, Samuel E.; Oh, Suh Yeon; Liu, Chen Science Advances, Vol. 6, Issue 10 https://doi.org/10.1126/sciadv.aaz0525	journal	March 2020
Potential of gene drives with genome editing to increase genetic gain in livestock breeding programs Gonen, Serap; Jenko, Janez; Gorjanc, Gregor Genetics Selection Evolution, Vol. 49, Issue 1 https://doi.org/10.1186/s12711-016-0280-3	journal	January 2017
Performance analysis of novel toxin-antidote CRISPR gene drive systems Champer, Jackson; Kim, Isabel K.; Champer, Samuel E. BMC Biology, Vol. 18, Issue 1 https://doi.org/10.1186/s12915-020-0761-2	journal	March 2020
The CRISPR revolution in fungal biology and biotechnology, and beyond Idnurm, Alexander; Meyer, Vera Fungal Biology and Biotechnology, Vol. 5, Issue 1 https://doi.org/10.1186/s40694-018-0064-3	journal	December 2018
Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae Goeckel, Megan E.; Basgall, Erianna M.; Lewis, Isabel C. Fungal Biology and Biotechnology, Vol. 6, Issue 1 https://doi.org/10.1186/s40694-019-0065-x	journal	February 2019
Progress towards engineering gene drives for population control Raban, Robyn R.; Marshall, John M.; Akbari, Omar S. The Journal of Experimental Biology, Vol. 223, Issue Suppl 1 https://doi.org/10.1242/jeb.208181	journal	February 2020
A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae Daniels, Peter W.; Mukherjee, Anuradha; Goldman, Alastair SH Wellcome Open Research, Vol. 3 https://doi.org/10.12688/wellcomeopenres.14642.2	journal	January 2018
Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts Mertens, Stijn; Gallone, Brigida; Steensels, Jan PLOS ONE, Vol. 14, Issue 1 https://doi.org/10.1371/journal.pone.0209124	journal	January 2019
Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae Roggenkamp, Emily; Giersch, Rachael M.; Schrock, Madison N. G3: Genes\|Genomes\|Genetics, Vol. 8, Issue 3 https://doi.org/10.1534/g3.117.300557	journal	January 2018
CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains Roggenkamp, Emily; Giersch, Rachael M.; Wedeman, Emily Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01773	journal	September 2017
An Overview of CRISPR-Based Tools and Their Improvements: New Opportunities in Understanding Plant–Pathogen Interactions for Better Crop Protection Barakate, Abdellah; Stephens, Jennifer Frontiers in Plant Science, Vol. 7 https://doi.org/10.3389/fpls.2016.00765	journal	June 2016
Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed? Macias, Vanessa; Ohm, Johanna; Rasgon, Jason International Journal of Environmental Research and Public Health, Vol. 14, Issue 9 https://doi.org/10.3390/ijerph14091006	journal	September 2017
On a Robust, Sensitive Cell-Free Method for Pseudomonas Sensing and Quantification in Microfluidic Templated Hydrogels Seto, Jong Micromachines, Vol. 10, Issue 8 https://doi.org/10.3390/mi10080506	journal	July 2019
Sustainability as a Framework for Considering Gene Drive Mice for Invasive Rodent Eradication Barnhill-Dilling, S.; Serr, Megan; Blondel, Dimitri Sustainability, Vol. 11, Issue 5 https://doi.org/10.3390/su11051334	journal	March 2019
A transatlantic perspective on 20 emerging issues in biological engineering Wintle, Bonnie C.; Boehm, Christian R.; Rhodes, Catherine eLife, Vol. 6 https://doi.org/10.7554/elife.30247	journal	November 2017
A Y-chromosome shredding gene drive for controlling pest vertebrate populations Prowse, Thomas AA; Adikusuma, Fatwa; Cassey, Phillip eLife, Vol. 8 https://doi.org/10.7554/elife.41873	journal	February 2019
Development of a confinable gene drive system in the human disease vector Aedes aegypti Li, Ming; Yang, Ting; Kandul, Nikolay P. eLife, Vol. 9 https://doi.org/10.7554/elife.51701	journal	January 2020
Towards the genetic control of invasive species Harvey-Samuel, Tim; Ant, Thomas; Alphey, Luke Biological Invasions, Vol. 19, Issue 6 https://doi.org/10.1007/s10530-017-1384-6	journal	February 2017
Scenario analysis on the use of rodenticides and sex-biasing gene drives for the removal of invasive house mice on islands Serr, Megan E.; Valdez, Rene X.; Barnhill-Dilling, Kathleen S. Biological Invasions, Vol. 22, Issue 4 https://doi.org/10.1007/s10530-019-02192-6	journal	January 2020
Welcome to the CRISPR zoo Reardon, Sara Nature, Vol. 531, Issue 7593 https://doi.org/10.1038/531160a	journal	March 2016
Applications of CRISPR technologies in research and beyond Barrangou, Rodolphe; Doudna, Jennifer A. Nature Biotechnology, Vol. 34, Issue 9 https://doi.org/10.1038/nbt.3659	journal	September 2016
A CRISPR–Cas9-based gene drive platform for genetic interaction analysis in Candida albicans Shapiro, Rebecca S.; Chavez, Alejandro; Porter, Caroline B. M. Nature Microbiology, Vol. 3, Issue 1 https://doi.org/10.1038/s41564-017-0043-0	journal	October 2017
Reducing resistance allele formation in CRISPR gene drive Champer, Jackson; Liu, Jingxian; Oh, Suh Yeon Proceedings of the National Academy of Sciences, Vol. 115, Issue 21 https://doi.org/10.1073/pnas.1720354115	journal	May 2018
The potential for the use of gene drives for pest control in New Zealand: a perspective Dearden, Peter K.; Gemmell, Neil J.; Mercier, Ocean R. Journal of the Royal Society of New Zealand, Vol. 48, Issue 4 https://doi.org/10.1080/03036758.2017.1385030	journal	October 2017
Harnessing gene drive Min, John; Smidler, Andrea L.; Najjar, Devora Journal of Responsible Innovation, Vol. 5, Issue sup1 https://doi.org/10.1080/23299460.2017.1415586	journal	December 2017
The ecology and evolution of microbial CRISPR-Cas adaptive immune systems Westra, Edze R.; van Houte, Stineke; Gandon, Sylvain Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 374, Issue 1772 https://doi.org/10.1098/rstb.2019.0101	journal	March 2019
Daisy-chain gene drives for the alteration of local populations Noble, Charleston; Min, John; Olejarz, Jason Proceedings of the National Academy of Sciences https://doi.org/10.1101/057307	journal	June 2016
Overcoming evolved resistance to population-suppressing homing-based gene drives Marshall, John M.; Buchman, Anna; Sanchez C., Hector M. Scientific Reports https://doi.org/10.1101/088427	journal	December 2016
Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs Oberhofer, Georg; Ivy, Tobin; Hay, Bruce A. Proceedings of the National Academy of Sciences https://doi.org/10.1101/289546	journal	March 2018
Development of a multi-locus CRISPR gene drive system in budding yeast Yan, Yao; Finnigan, Gregory C. Scientific Reports https://doi.org/10.1101/391334	journal	August 2018
The memory of hyperosmotic stress response in yeast is modulated by gene-positioning Ben Meriem, Zacchari; Khalil, Yasmine; Hersen, Pascal Cells https://doi.org/10.1101/625756	journal	May 2019
A toxin-antidote CRISPR gene drive system for regional population modification Champer, Jackson; Lee, Yoo Lim; Yang, Emily Nature Communications https://doi.org/10.1101/628354	journal	May 2019
Resistance is futile: A CRISPR homing gene drive targeting a haplolethal gene Champer, Jackson; Yang, Emily; Lee, Yoo Lim Proceedings of the National Academy of Sciences https://doi.org/10.1101/651737	journal	May 2019
Designing gene drives to limit spillover to non-target populations Greenbaum, Gili; Feldman, Marcus W.; Rosenberg, Noah A. PLOS Genetics, Vol. 17, Issue 2 https://doi.org/10.1371/journal.pgen.1009278	journal	February 2021
Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes Carvalho, Danilo O.; McKemey, Andrew R.; Garziera, Luiza PLOS Neglected Tropical Diseases, Vol. 9, Issue 7 https://doi.org/10.1371/journal.pntd.0003864	journal	July 2015
The CRISPR toolbox in medical mycology: State of the art and perspectives Morio, Florent; Lombardi, Lisa; Butler, Geraldine PLOS Pathogens, Vol. 16, Issue 1 https://doi.org/10.1371/journal.ppat.1008201	journal	January 2020
mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence Finnigan, Gregory C.; Thorner, Jeremy G3 Genes\|Genomes\|Genetics, Vol. 6, Issue 7 https://doi.org/10.1534/g3.116.029801	journal	July 2016
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae. Hammond, Andrew; Galizi, Roberto; Kyrou, Kyros Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.21692	text	January 2016
Gene Disruption Technologies Have the Potential to Transform Stored Product Insect Pest Control Perkin, Lindsey; Adrianos, Sherry; Oppert, Brenda Insects, Vol. 7, Issue 3 https://doi.org/10.3390/insects7030046	journal	September 2016
Molecular safeguarding of CRISPR gene drive experiments Champer, Jackson; Chung, Joan; Lee, Yoo Lim eLife, Vol. 8 https://doi.org/10.7554/eLife.41439	journal	January 2019
A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment López Del Amo, Víctor; Bishop, Alena L.; Sánchez C., Héctor M. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13977-7	journal	January 2020
Viral gene drive in herpesviruses Walter, Marius; Verdin, Eric Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-18678-0	journal	September 2020
Daisy-chain gene drives for the alteration of local populations Noble, Charleston; Min, John; Olejarz, Jason Proceedings of the National Academy of Sciences, Vol. 116, Issue 17 https://doi.org/10.1073/pnas.1716358116	journal	April 2019
Anticipating complexity in the deployment of gene drive insects in agriculture Baltzegar, Jennifer; Barnes, Jessica Cavin; Elsensohn, Johanna E. Journal of Responsible Innovation https://doi.org/10.1101/169938	journal	July 2017
Current CRISPR gene drive systems are likely to be highly invasive in wild populations Noble, Charleston; Adlam, Ben; Church, George M. eLife https://doi.org/10.1101/219022	journal	November 2017
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast Buchmuller, Benjamin C.; Herbst, Konrad; Meurer, Matthias Nature Communications https://doi.org/10.1101/476804	journal	June 2019
Designing gene drives to limit spillover to non-target populations Greenbaum, Gili; Feldman, Marcus W.; Rosenberg, Noah A. PLOS Genetics https://doi.org/10.1101/680744	journal	August 2019
A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae Daniels, Peter W.; Mukherjee, Anuradha; Goldman, Alastair SH Wellcome Open Research, Vol. 3 https://doi.org/10.12688/wellcomeopenres.14642.1	journal	January 2018
A transatlantic perspective on 20 emerging issues in biological engineering Wintle, Bonnie C.; Boehm, Christian; Rhodes, Catherine Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.20852	text	January 2017
Overcoming evolved resistance to population-suppressing homing-based gene drives Marshall, John M.; Buchman, Anna; Sánchez C., Héctor M. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-02744-7	journal	June 2017
Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae Goeckel, Megan E.; Basgall, Erianna M.; Lewis, Isabel C. Fungal Biology and Biotechnology https://doi.org/10.1101/408369	journal	September 2018
Molecular safeguarding of CRISPR gene drive experiments Champer, Jackson; Chung, Joan; Lee, Yoo Lim eLife https://doi.org/10.1101/411876	journal	September 2018
Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs Champer, Samuel E.; Oh, Suh Yeon; Liu, Chen Science Advances https://doi.org/10.1101/679902	journal	June 2019
Current CRISPR gene drive systems are likely to be highly invasive in wild populations Noble, Charleston; Adlam, Ben; Church, George M. eLife, Vol. 7 https://doi.org/10.7554/elife.33423	journal	June 2018
Optimizing the DNA Donor Template for Homology-Directed Repair of Double-Strand Breaks Song, Fei; Stieger, Knut Molecular Therapy - Nucleic Acids, Vol. 7 https://doi.org/10.1016/j.omtn.2017.02.006	journal	June 2017
Fundamental CRISPR-Cas9 tools and current applications in microbial systems Tian, Pingfang; Wang, Jia; Shen, Xiaolin Synthetic and Systems Biotechnology, Vol. 2, Issue 3 https://doi.org/10.1016/j.synbio.2017.08.006	journal	September 2017
Daisyfield gene drive systems harness repeated genomic elements as a generational clock to limit spread Min, John; Noble, Charleston; Najjar, Devora BioRxiv https://doi.org/10.1101/104877	journal	February 2017
Daisy quorum drives for the genetic restoration of wild populations Min, John; Noble, Charleston; Najjar, Devora BioRxiv https://doi.org/10.1101/115618	posted_content	March 2017
Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology Marshall, John M.; Akbari, Omar S. ACS Chemical Biology https://doi.org/10.1101/173914	journal	August 2017
Can a population targeted by a CRISPR-based homing gene drive be rescued? Rode, Nicolas O.; Courtier-Orgogozo, Virginie; Debarre, Florence G3: Genes\|Genomes\|Genetics https://doi.org/10.1101/2020.03.17.995829	journal	July 2020
Evaluating the Probability of CRISPR-based Gene Drive Contaminating Another Species Courtier-Orgogozo, Virginie; Danchin, Antoine; Gouyon, Pierre-Henri Evolutionary Applications https://doi.org/10.1101/776609	journal	September 2019
CRISPR/Cas9: the Jedi against the dark empire of diseases Khan, Sehrish; Mahmood, Muhammad Shahid; Rahman, Sajjad ur Journal of Biomedical Science, Vol. 25, Issue 1 https://doi.org/10.1186/s12929-018-0425-5	journal	March 2018
Gene drives as a response to infection and resistance Hayirli, Tuna C.; Martelli, Peter F. Infection and Drug Resistance, Vol. Volume 12 https://doi.org/10.2147/idr.s187424	journal	January 2019
Evolutionary Genetic Engineering in the Indo-Pacific: Conservation, Humanitarian, and Social Issues Reed, Floyd A. arXiv https://doi.org/10.48550/arxiv.1706.01710	preprint	January 2017

Similar Records

Epigenetic Footprints of CRISPR/Cas9-Mediated Genome Editing in Plants

Journal Article · Fri Jan 31 00:00:00 EST 2020 · Frontiers in Plant Science · OSTI ID:1466972

Lee, Jun Hyung; Mazarei, Mitra; Pfotenhauer, Alexander; +6 more

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

Journal Article · Mon Mar 04 00:00:00 EST 2013 · Nucleic Acids Research · OSTI ID:1466972

DiCarlo, James E.; Norville, Julie E.; Mali, Prashant; +3 more

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Journal Article · Thu Jun 23 00:00:00 EDT 2016 · Microbial Cell Factories · OSTI ID:1466972

Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Safeguarding CRISPR-Cas9 gene drives in yeast

Citation Formats

References (36)

Cited By (101)

Similar Records

Related Subjects