Compositions and methods for making selenocysteine containing polypeptides

Soll, Dieter; Aldag, Caroline; Hohn, Michael

Title: Compositions and methods for making selenocysteine containing polypeptides

Patent · Tue Jul 17 00:00:00 EDT 2018

OSTI ID:1466033

Soll, Dieter; Aldag, Caroline; Hohn, Michael

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA^Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA^Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA^Sec.

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Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-98ER20311

Assignee:: Yale University (New Haven, CT)

Patent Number(s):: 10,023,893

Application Number:: 15/274,541

OSTI ID:: 1466033

Resource Relation:: Patent File Date: 2016 Sep 23

Country of Publication:: United States

Language:: English

References (179)

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons Ambrogelly, A.; Gundllapalli, S.; Herring, S. Proceedings of the National Academy of Sciences, Vol. 104, Issue 9, p. 3141-3146 https://doi.org/10.1073/pnas.0611634104	journal	February 2007
Crystal Structure of the Ternary Complex of Phe-tRNA^Phe, EF-Tu, and a GTP Analog Nissen, Poul; Kjeldgaard, Morten; Thirup, Søren Science, Vol. 270, Issue 5241, p. 1464-1472 https://doi.org/10.1126/science.270.5241.1464	journal	December 1995
Emergence of the universal genetic code imprinted in an RNA record Hohn, M. J.; Park, H.-S.; O'Donoghue, P. Proceedings of the National Academy of Sciences, Vol. 103, Issue 48, p. 18095-18100 https://doi.org/10.1073/pnas.0608762103	journal	November 2006
A Hypergeometric Probability Model for Protein Identification and Validation Using Tandem Mass Spectral Data and Protein Sequence Databases Sadygov, Rovshan G.; Yates, John R. Analytical Chemistry, Vol. 75, Issue 15, p. 3792-3798 https://doi.org/10.1021/ac034157w	journal	August 2003
Efficient Expression of Glutathione Peroxidase with Chimeric tRNA in Amber-less Escherichia coli Fan, Zhenlin; Song, Jian; Guan, Tuchen ACS Synthetic Biology, Vol. 7, Issue 1, p. 249-257 https://doi.org/10.1021/acssynbio.7b00290	journal	August 2017
Evolution of the selenoproteome in Helicobacter pylori and Epsilonproteobacteria Cravedi, P.; Mori, G.; Fischer, F. Genome Biology and Evolution https://doi.org/10.1093/gbe/evv177	journal	September 2015
NMR and biochemical characterization of recombinant human tRNA₃^Lys expressed in Escherichia coli: Identification of posttranscriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription Tisné, Carine; Rigourd, Mickaël; Marquet, Roland RNA, Vol. 6, Issue 10, p. 1403-1412 https://doi.org/10.1017/S1355838200000947	journal	October 2000
The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus. Engelhard, E. K.; Kam-Morgan, L. N.; Washburn, J. O. Proceedings of the National Academy of Sciences, Vol. 91, Issue 8, p. 3224-3227 https://doi.org/10.1073/pnas.91.8.3224	journal	April 1994
Rewiring Translation for Elongation Factor Tu-Dependent Selenocysteine Incorporation Aldag, Caroline; Bröcker, Markus J.; Hohn, Michael J. Angewandte Chemie International Edition, Vol. 52, Issue 5, p. 1441-1445 https://doi.org/10.1002/anie.201207567	journal	November 2012
Selenoglutathione: Efficient Oxidative Protein Folding by a Diselenide Beld, Joris; Woycechowsky, Kenneth J.; Hilvert, Donald Biochemistry, Vol. 46, Issue 18, p. 5382-5390 https://doi.org/10.1021/bi700124p	journal	May 2007
The Affinity of Elongation Factor Tu for an Aminoacyl-tRNA Is Modulated by the Esterified Amino Acid Dale, Taraka; Sanderson, Lee E.; Uhlenbeck, Olke C. Biochemistry, Vol. 43, Issue 20, p. 6159-6166 https://doi.org/10.1021/bi036290o	journal	May 2004
Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation LaRiviere, Frederick J.; Wolfson, Alexey D.; Uhlenbeck, Olke C. Science, Vol. 294, Issue 5540, p. 165-168 https://doi.org/10.1126/science.1064242	journal	October 2001
In vivo incorporation of unnatural amino acids Schultz, Peter; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030082575 US Patent Application 10/126927; 20030082575	patent-application	May 2003
Fast purification of a functional elongator tRNA^met expressed from a synthetic gene in vivo Meinnel, Thierry; Mechulam, Yves; Fayat, Guy Nucleic Acids Research, Vol. 16, Issue 16, p. 8095-8112 https://doi.org/10.1093/nar/16.16.8095	journal	January 1988
Drag&Drop cloning in yeast Jansen, Gregor; Wu, Cunle; Schade, Babette Gene, Vol. 344, p. 43-51 https://doi.org/10.1016/j.gene.2004.10.016	journal	January 2005
Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function Jacob, Claus; Giles, Gregory I.; Giles, Niroshini M. Angewandte Chemie International Edition, Vol. 42, Issue 39, p. 4742-4758 https://doi.org/10.1002/anie.200300573	journal	October 2003
Decameric SelA•tRNA^Sec Ring Structure Reveals Mechanism of Bacterial Selenocysteine Formation Itoh, Y.; Brocker, M. J.; Sekine, S.-i. Science, Vol. 340, Issue 6128, p. 75-78 https://doi.org/10.1126/science.1229521	journal	April 2013
One Polypeptide with Two Aminoacyl-tRNA Synthetase Activities Stathopoulos, Constantino; Li, Tong; Longman, Randy Science, Vol. 287, Issue 5452, p. 479-482 https://doi.org/10.1126/science.287.5452.479	journal	January 2000
Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-tRNA synthetase Soll, Dieter; Rinehart, Jesse https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7723069 US Patent Document 7,723,069	patent	May 2010
The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P. Orellana, O.; Cooley, L.; Söll, D. Molecular and Cellular Biology, Vol. 6, Issue 2, p. 525-529 https://doi.org/10.1128/MCB.6.2.525	journal	February 1986
Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end McClain, W.; Foss, K. Science, Vol. 240, Issue 4853, p. 793-796 https://doi.org/10.1126/science.2452483	journal	May 1988
Phenylalanyl-tRNA synthetase editing defects result in efficient mistranslation of phenylalanine codons as tyrosine Ling, J.; Yadavalli, S. S.; Ibba, M. RNA, Vol. 13, Issue 11, p. 1881-1886 https://doi.org/10.1261/rna.684107	journal	September 2007
Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and an Fe₄S₄ Cluster Boyington, Jeffrey C.; Gladyshev, Vadim N.; Khangulov, Sergei V. Science, Vol. 275, Issue 5304, p. 1305-1308 https://doi.org/10.1126/science.275.5304.1305	journal	February 1997
Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection. Logan, J.; Shenk, T. Proceedings of the National Academy of Sciences, Vol. 81, Issue 12, p. 3655-3659 https://doi.org/10.1073/pnas.81.12.3655	journal	June 1984
Caged Phosphoproteins Rothman, Deborah M.; Petersson, E. James; Vázquez, M. Eugenio Journal of the American Chemical Society, Vol. 127, Issue 3, p. 846-847 https://doi.org/10.1021/ja043875c	journal	January 2005
The unusual methanogenic seryl‐tRNA synthetase recognizes tRNA^Ser species from all three kingdoms of life Bilokapic, Silvija; Korencic, Dragana; Söll, Dieter European Journal of Biochemistry, Vol. 271, Issue 4, p. 694-702 https://doi.org/10.1111/j.1432-1033.2003.03971.x	journal	January 2004
Manipulating Cellular Activities Using an Ultrasound–Chemical Hybrid Tool Fan, Ching-Hsiang; Huang, Yao-Shen; Huang, Wei-En ACS Synthetic Biology, Vol. 6, Issue 11, p. 2021-2027 https://doi.org/10.1021/acssynbio.7b00162	journal	August 2017
Silvibacterium bohemicum gen. nov. sp. nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park Lladó, Salvador; Benada, Oldrich; Cajthaml, Tomáš Systematic and Applied Microbiology, Vol. 39, Issue 1, p. 14-19 https://doi.org/10.1016/j.syapm.2015.12.005	journal	February 2016
Formation of a Ternary Complex for Selenocysteine Biosynthesis in Bacteria Silva, Ivan R.; Serrão, Vitor H. B.; Manzine, Livia R. Journal of Biological Chemistry, Vol. 290, Issue 49, p. 29178-29188 https://doi.org/10.1074/jbc.M114.613406	journal	September 2015
Changing the identity of a transfer RNA Normanly, Jennifer; Ogden, Richard C.; Horvath, Suzanna J. Nature, Vol. 321, Issue 6067, p. 213-219 https://doi.org/10.1038/321213a0	journal	May 1986
Formation of de novo centromeres and construction of first-generation human artificial microchromosomes Harrington, John J.; Bokkelen, Gil Van; Mays, Robert W. Nature Genetics, Vol. 15, Issue 4, p. 345-355 https://doi.org/10.1038/ng0497-345	journal	April 1997
The length and the secondary structure of the D-stem of human selenocysteine tRNA are the major identity determinants for serine phosphorylation. Wu, X. Q.; Gross, H. J. The EMBO Journal, Vol. 13, Issue 1 https://doi.org/10.1002/j.1460-2075.1994.tb06254.x	journal	January 1994
The Adaptor hypothesis revisited Ibba, Michael; Becker, Hubert D.; Stathopoulos, Constantinos Trends in Biochemical Sciences, Vol. 25, Issue 7, p. 311-316 https://doi.org/10.1016/S0968-0004(00)01600-5	journal	July 2000
Targeting the mitogen-activated protein kinase cascade to treat cancer Sebolt-Leopold, Judith S.; Herrera, Roman Nature Reviews Cancer, Vol. 4, Issue 12, p. 937-947 https://doi.org/10.1038/nrc1503	journal	December 2004
Five-base codons for incorporation of nonnatural amino acids into proteins Hohsaka, Takahiro; Ashizuka, Yuki; Murakami, Hiroshi Nucleic Acids Research, Vol. 29, Issue 17, p. 3646-3651 https://doi.org/10.1093/nar/29.17.3646	journal	September 2001
Clustal W and Clustal X version 2.0 Larkin, M. A.; Blackshields, G.; Brown, N. P. Bioinformatics, Vol. 23, Issue 21, p. 2947-2948 https://doi.org/10.1093/bioinformatics/btm404	journal	September 2007
DTASelect and Contrast: Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics Tabb, David L.; McDonald, W. Hayes; Yates, John R. Journal of Proteome Research, Vol. 1, Issue 1, p. 21-26 https://doi.org/10.1021/pr015504q	journal	February 2002
Transfer RNAs with novel cloverleaf structures Mukai, Takahito; Vargas-Rodriguez, Oscar; Englert, Markus Nucleic Acids Research https://doi.org/10.1093/nar/gkw898	journal	October 2016
[15] Biosynthetic method for introducing unnatural amino acids site-specifically into proteins Ellman, Jon; Mendel, David; Anthony-Cahill, Spencer Molecular Design and Modeling: Concepts and Applications Part A: Proteins, Peptides, and Enzymes, p. 301-336 https://doi.org/10.1016/0076-6879(91)02017-4	book	January 1991
Noncanonical Secondary Structure Stabilizes Mitochondrial tRNA^Ser(UCN) by Reducing the Entropic Cost of Tertiary Folding Mustoe, Anthony M.; Liu, Xin; Lin, Paul J. Journal of the American Chemical Society, Vol. 137, Issue 10, p. 3592-3599 https://doi.org/10.1021/ja5130308	journal	March 2015
Global analysis of a “simple” proteome: Methanococcus jannaschii Giometti, Carol S.; Reich, Claudia; Tollaksen, Sandra Journal of Chromatography B, Vol. 782, Issue 1-2, p. 227-243 https://doi.org/10.1016/S1570-0232(02)00568-8	journal	December 2002
Modulation of tRNA^Ala identity by inorganic pyrophosphatase Wolfson, A. D.; Uhlenbeck, O. C. Proceedings of the National Academy of Sciences, Vol. 99, Issue 9, p. 5965-5970 https://doi.org/10.1073/pnas.092152799	journal	April 2002
Recognition of tRNA^Cys by Escherichia coli cysteinyl-tRNA synthetase Komatsoulis, George A.; Abelson, John Biochemistry, Vol. 32, Issue 29, p. 7435-7444 https://doi.org/10.1021/bi00080a014	journal	July 1993
Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. Alessi, D. R.; Saito, Y.; Campbell, D. G. The EMBO Journal, Vol. 13, Issue 7 https://doi.org/10.1002/j.1460-2075.1994.tb06424.x	journal	April 1994
Synthesis of Cysteinyl-tRNA^Cys by a Genome That Lacks the Normal Cysteine-tRNA Synthetase Lipman, Richard S. A.; Sowers, Kevin R.; Hou, Ya-Ming Biochemistry, Vol. 39, Issue 26, p. 7792-7798 https://doi.org/10.1021/bi0004955	journal	July 2000
Dynamics of Recognition between tRNA and Elongation Factor Tu Eargle, John; Black, Alexis A.; Sethi, Anurag Journal of Molecular Biology, Vol. 377, Issue 5, p. 1382-1405 https://doi.org/10.1016/j.jmb.2008.01.073	journal	April 2008
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
The ‘polysemous’ codon—a codon with multiple amino acid assignment caused by dual specificity of tRNA identity Suzuki, Tsutomu; Ueda, Takuya; Watanabe, Kimitsuna The EMBO Journal, Vol. 16, Issue 5, p. 1122-1134 https://doi.org/10.1093/emboj/16.5.1122	journal	March 1997
Genetic Codes with No Dedicated Stop Codon: Context-Dependent Translation Termination Swart, Estienne Carl; Serra, Valentina; Petroni, Giulio Cell, Vol. 166, Issue 3, p. 691-702 https://doi.org/10.1016/j.cell.2016.06.020	journal	July 2016
Genetic Code Supports Targeted Insertion of Two Amino Acids by One Codon Turanov, A. A.; Lobanov, A. V.; Fomenko, D. E. Science, Vol. 323, Issue 5911, p. 259-261 https://doi.org/10.1126/science.1164748	journal	January 2009
New Developments in Selenium Biochemistry: Selenocysteine Biosynthesis in Eukaryotes and Archaea Xu, Xue-Ming; Carlson, Bradley A.; Zhang, Yan Biological Trace Element Research, Vol. 119, Issue 3, p. 234-241 https://doi.org/10.1007/s12011-007-8003-9	journal	August 2007
The pathway to GTPase activation of elongation factor SelB on the ribosome Fischer, Niels; Neumann, Piotr; Bock, Lars V. Nature, Vol. 540, Issue 7631, p. 80-85 https://doi.org/10.1038/nature20560	journal	November 2016
Comparison of biosequences Smith, Temple F.; Waterman, Michael S. Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489 https://doi.org/10.1016/0196-8858(81)90046-4	journal	December 1981
Large Multiple Organism Gene Finding by Collapsed Gibbs Sampling Chatterji, Sourav; Pachter, Lior Journal of Computational Biology, Vol. 12, Issue 6, p. 599-608 https://doi.org/10.1089/cmb.2005.12.599	journal	July 2005
Compositions and methods for making selenocysteine containing polypeptides Soll, Dieter; Aldag, Caroline; Hohn, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9464288 US Patent Document 9,464,288	patent	October 2016
The antioxidant role of selenium and seleno-compounds Tapiero, H.; Townsend, D. M.; Tew, K. D. Biomedicine & Pharmacotherapy, Vol. 57, Issue 3-4, p. 134-144 https://doi.org/10.1016/S0753-3322(03)00035-0	journal	May 2003
A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies Buchner, Johannes; Pastan, Ira; Brinkmann, Ulrich Analytical Biochemistry, Vol. 205, Issue 2, p. 263-270 https://doi.org/10.1016/0003-2697(92)90433-8	journal	September 1992
Probability-Based Validation of Protein Identifications Using a Modified SEQUEST Algorithm MacCoss, Michael J.; Wu, Christine C.; Yates, John R. Analytical Chemistry, Vol. 74, Issue 21, p. 5593-5599 https://doi.org/10.1021/ac025826t	journal	November 2002
Cys-tRNA^Cys formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem? Ambrogelly, A.; Kamtekar, S.; Sauerwald, A. Cellular and Molecular Life Sciences, Vol. 61, Issue 19-20, p. 2437-2445 https://doi.org/10.1007/s00018-004-4194-9	journal	October 2004
Evolving tRNA^Sec for Efficient Canonical Incorporation of Selenocysteine Thyer, Ross; Robotham, Scott A.; Brodbelt, Jennifer S. Journal of the American Chemical Society, Vol. 137, Issue 1, p. 46-49 https://doi.org/10.1021/ja510695g	journal	December 2014
RNA-Dependent Cysteine Biosynthesis in Archaea Sauerwald, Anselm; Zhu, Wenhong; Major, Tiffany A. Science, Vol. 307, Issue 5717, p. 1969-1972 https://doi.org/10.1126/science.1108329	journal	March 2005
Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology Katz, Assaf; Elgamal, Sara; Rajkovic, Andrei Molecular Microbiology, Vol. 101, Issue 4, p. 545-558 https://doi.org/10.1111/mmi.13419	journal	June 2016
Gene Transfer into Hepatocytes and Human Liver Tissue by Baculovirus Vectors Sandig, Volker; Hofmann, Christian; Steinert, Susanne Human Gene Therapy, Vol. 7, Issue 16, p. 1937-1945 https://doi.org/10.1089/hum.1996.7.16-1937	journal	October 1996
Understanding the Sequence Specificity of tRNA Binding to Elongation Factor Tu using tRNA Mutagenesis Schrader, Jared M.; Chapman, Stephen J.; Uhlenbeck, Olke C. Journal of Molecular Biology, Vol. 386, Issue 5, p. 1255-1264 https://doi.org/10.1016/j.jmb.2009.01.021	journal	March 2009
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Datsenko, K. A.; Wanner, B. L. Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645 https://doi.org/10.1073/pnas.120163297	journal	May 2000
Selected polynucleotide and polypeptide sequences of the methanogenic archaeon, methanococcus jannashii Bult, Carol J.; White, Owen; Smith, Hamilton O. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6503729 US Patent Document 6,503,729	patent	January 2003
Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA Takamatsu, Nobuhiko; Ishikawa, Masayuki; Meshi, Tetsuo The EMBO Journal, Vol. 6, Issue 2 https://doi.org/10.1002/j.1460-2075.1987.tb04755.x	journal	February 1987
Sequence Divergence of Seryl-tRNA Synthetases in Archaea Kim, Hyun-soo; Vothknecht, Ute C.; Hedderich, Reiner Journal of Bacteriology, Vol. 180, Issue 24 https://doi.org/10.1128/JB.180.24.6446-6449.1998	journal	December 1998
Phenylalanyl-tRNA synthetase from the archaeon Methanobacterium thermoautotrophicum is an (αβ)₂ heterotetrameric protein Das, Rajdeep; Vothknecht, Ute C. Biochimie, Vol. 81, Issue 11, p. 1037-1039 https://doi.org/10.1016/S0300-9084(99)00332-6	journal	November 1999
A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro Miller, Corwin; Bröcker, Markus J.; Prat, Laure FEBS Letters, Vol. 589, Issue 17, p. 2194-2199 https://doi.org/10.1016/j.febslet.2015.06.039	journal	July 2015
Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation Min, B.; Pelaschier, J. T.; Graham, D. E. Proceedings of the National Academy of Sciences, Vol. 99, Issue 5, p. 2678-2683 https://doi.org/10.1073/pnas.012027399	journal	March 2002
Diselenium, instead of disulfide, bonded analogs of conotoxins: novel synthesis and pharmacotherapeutic potential Raffa, Robert B. Life Sciences, Vol. 87, Issue 15-16, p. 451-456 https://doi.org/10.1016/j.lfs.2010.07.011	journal	October 2010
PSI-BLAST-ISS: an intermediate sequence search tool for estimation of the position-specific alignment reliability Margelevičius, Mindaugas; Venclovas, Česlovas BMC Bioinformatics, Vol. 6, Issue 1, Article No. 185 https://doi.org/10.1186/1471-2105-6-185	journal	January 2005
Positional control of selenium insertion in polypeptides for X-ray crystallography Leonard, Jack L.; Newburger, Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5700660 US Patent Document 5,700,660	patent	December 1997
Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement Isaacs, Farren J.; Carr, Peter A.; Wang, Harris H. Science, Vol. 333, Issue 6040, p. 348-353 https://doi.org/10.1126/science.1205822	journal	July 2011
Transformation of Methanococcus maripaludis and identification of a Pst I-like restriction system Tumbula, Debra L.; Makula, Ronald A.; Whitman, William B. FEMS Microbiology Letters, Vol. 121, Issue 3, p. 309-314 https://doi.org/10.1111/j.1574-6968.1994.tb07118.x	journal	September 1994
Pathogenic mechanism of a human mitochondrial tRNA^Phe mutation associated with myoclonic epilepsy with ragged red fibers syndrome Ling, J.; Roy, H.; Qin, D. Proceedings of the National Academy of Sciences, Vol. 104, Issue 39, p. 15299-15304 https://doi.org/10.1073/pnas.0704441104	journal	September 2007
Alternative genetic code for amino acids and transfer RNA revisited Hamashima, Kiyofumi; Kanai, Akio BioMolecular Concepts, Vol. 4, Issue 3, p. 309-318 https://doi.org/10.1515/bmc-2013-0002	journal	January 2013
Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine Kim, Moon-Jung; Lee, Byung Cheon; Hwang, Kwang Yeon Biochemical and Biophysical Research Communications, Vol. 461, Issue 4, p. 648-652 https://doi.org/10.1016/j.bbrc.2015.04.082	journal	June 2015
Adaptation to tRNA acceptor stem structure by flexible adjustment in the catalytic domain of class I tRNA synthetases Liu, C.; Sanders, J. M.; Pascal, J. M. RNA, Vol. 18, Issue 2, p. 213-221 https://doi.org/10.1261/rna.029983.111	journal	December 2011
Expanding the Genetic Code of Escherichia coli with Phosphoserine Park, H.-S.; Hohn, M. J.; Umehara, T. Science, Vol. 333, Issue 6046, p. 1151-1154 https://doi.org/10.1126/science.1207203	journal	August 2011
Tertiary structure of bacterial selenocysteine tRNA Itoh, Y.; Sekine, S.-i.; Suetsugu, S. Nucleic Acids Research, Vol. 41, Issue 13, p. 6729-6738 https://doi.org/10.1093/nar/gkt321	journal	May 2013
Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems Yuan, Jing; O'Donoghue, Patrick; Ambrogelly, Alex FEBS Letters, Vol. 584, Issue 2, p. 342-349 https://doi.org/10.1016/j.febslet.2009.11.005	journal	November 2009
RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea Yuan, J.; Palioura, S.; Salazar, J. C. Proceedings of the National Academy of Sciences, Vol. 103, Issue 50, p. 18923-18927 https://doi.org/10.1073/pnas.0609703104	journal	December 2006
Recombinant Expression of Mammalian Selenocysteine-Containing Thioredoxin Reductase and Other Selenoproteins in Escherichia coli Arnér, Elias S .J. Protein Sensors and Reactive Oxygen Species - Part A: Selenoproteins and Thioredoxin https://doi.org/10.1016/S0076-6879(02)47022-X	book	January 2002
An integrated cell-free metabolic platform for protein production and synthetic biology Jewett, Michael C.; Calhoun, Kara A.; Voloshin, Alexei Molecular Systems Biology, Vol. 4, Issue 1, Article No. 220 https://doi.org/10.1038/msb.2008.57	journal	October 2008
Protein production by auto-induction in high-density shaking cultures Studier, F. William Protein Expression and Purification, Vol. 41, Issue 1, p. 207-234 https://doi.org/10.1016/j.pep.2005.01.016	journal	May 2005
RNA-Dependent Cysteine Biosynthesis in Bacteria and Archaea Mukai, Takahito; Crnković, Ana; Umehara, Takuya mBio, Vol. 8, Issue 3, Article No. e00561-17 https://doi.org/10.1128/mBio.00561-17	journal	May 2017
Crystal structure of human selenocysteine tRNA Itoh, Yuzuru; Chiba, Shiho; Sekine, Shun-ichi Nucleic Acids Research, Vol. 37, Issue 18, p. 6259-6268 https://doi.org/10.1093/nar/gkp648	journal	August 2009
Ribosomal synthesis of nonstandard peptides Kang, Taek Jin; Suga, Hiroaki Biochemistry and Cell Biology, Vol. 86, Issue 2, p. 92-99 https://doi.org/10.1139/O08-009	journal	April 2008
Selenite Reduction by the Thioredoxin System: Kinetics and Identification of Protein-Bound Selenide Tamura, Takashi; Sato, Kumi; Komori, Kentaro Bioscience, Biotechnology, and Biochemistry, Vol. 75, Issue 6, p. 1184-1187 https://doi.org/10.1271/bbb.100847	journal	June 2011
Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase Holland, Michael J.; Holland, Janice P. Biochemistry, Vol. 17, Issue 23, p. 4900-4907 https://doi.org/10.1021/bi00616a007	journal	November 1978
Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine Aldag, C.; Gromov, I. A.; Garcia-Rubio, I. Proceedings of the National Academy of Sciences, Vol. 106, Issue 14, p. 5481-5486 https://doi.org/10.1073/pnas.0810503106	journal	March 2009
Characterization of Mammalian Selenoproteomes Kryukov, Gregory V.; Castellano, Sergi; Novoselov, Sergey V. Science, Vol. 300, Issue 5624, p. 1439-1443 https://doi.org/10.1126/science.1083516	journal	May 2003
The Catalytic Properties of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis Calendar, Richard; Berg, Paul Biochemistry, Vol. 5, Issue 5, p. 1690-1695 https://doi.org/10.1021/bi00869a034	journal	May 1966
Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu. Rudinger, J.; Hillenbrandt, R.; Sprinzl, M. The EMBO Journal, Vol. 15, Issue 3 https://doi.org/10.1002/j.1460-2075.1996.tb00397.x	journal	February 1996
Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis Calendar, Richard; Berg, Paul Biochemistry, Vol. 5, Issue 5, p. 1681-1690 https://doi.org/10.1021/bi00869a033	journal	May 1966
A novel nuclear genetic code alteration in yeasts and the evolution of codon reassignment in eukaryotes Mühlhausen, Stefanie; Findeisen, Peggy; Plessmann, Uwe Genome Research, Vol. 26, Issue 7, p. 945-955 https://doi.org/10.1101/gr.200931.115	journal	May 2016
ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences Laslett, Dean; Canback, Bjorn Nucleic Acids Research, Vol. 32, Issue 1, p. 11-16 https://doi.org/10.1093/nar/gkh152	journal	January 2004
Positive-selection vectors using the F plasmid ccdB killer gene Bernard, Philippe; Gabarit, Philippe; Bahassi, El Mustapha Gene, Vol. 148, Issue 1, p. 71-74 https://doi.org/10.1016/0378-1119(94)90235-6	journal	October 1994
Usage of an intronic promoter for stable gene expression in Saccharomyces cerevisiae Li, L.; Shen, S.; Jiang, P. Letters in Applied Microbiology, Vol. 40, Issue 5, p. 347-352 https://doi.org/10.1111/j.1472-765X.2005.01691.x	journal	May 2005
Genomically Recoded Organisms Expand Biological Functions Lajoie, M. J.; Rovner, A. J.; Goodman, D. B. Science, Vol. 342, Issue 6156, p. 357-360 https://doi.org/10.1126/science.1241459	journal	October 2013
Site-specific incorporation of phosphoserine into proteins in Escherichia coli Park, Hee-Sung; Soll, Dieter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9090928 US Patent Document 9,090,928	patent	July 2015
Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation Sherrer, R. Lynn; O’Donoghue, Patrick; Söll, Dieter Nucleic Acids Research, Vol. 36, Issue 4, p. 1247-1259 https://doi.org/10.1093/nar/gkm1134	journal	January 2008
The dual identities of mammalian tRNASec for SerRS and selenocysteine synthase Mizutani, Takaharu; Kanaya, Kazuo; Ikeda, Seiei Molecular Biology Reports, Vol. 25, Issue 4, p. 211-216 https://doi.org/10.1023/A:1006879820805	journal	January 1998
A proteomic view of the Plasmodium falciparum life cycle Florens, Laurence; Washburn, Michael P.; Raine, J. Dale Nature, Vol. 419, Issue 6906, p. 520-526 https://doi.org/10.1038/nature01107	journal	October 2002
The selenocysteine-inserting opal suppressor serine tRNA from E.coli is highly unusual in structure and modification Schön, Astrid; Böck, August; Ott, Günther Nucleic Acids Research, Vol. 17, Issue 18, p. 7159-7165 https://doi.org/10.1093/nar/17.18.7159	journal	January 1989
Engineering the elongation factor Tu for efficient selenoprotein synthesis Haruna, Ken-ichi; Alkazemi, Muhammad H.; Liu, Yuchen Nucleic Acids Research, Vol. 42, Issue 15, p. 9976-9983 https://doi.org/10.1093/nar/gku691	journal	July 2014
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes Xu, Xue-Ming; Carlson, Bradley A; Mix, Heiko PLoS Biology, Vol. 5, Issue 1, Article No. e4 https://doi.org/10.1371/journal.pbio.0050004	journal	December 2006
Stop codon reassignments in the wild Ivanova, N. N.; Schwientek, P.; Tripp, H. J. Science, Vol. 344, Issue 6186, p. 909-913 https://doi.org/10.1126/science.1250691	journal	May 2014
Facile Recoding of Selenocysteine in Nature Mukai, Takahito; Englert, Markus; Tripp, H. James Angewandte Chemie International Edition, Vol. 55, Issue 17, p. 5337-5341 https://doi.org/10.1002/anie.201511657	journal	March 2016
The discriminator base influences tRNA structure at the end of the acceptor stem and possibly its interaction with proteins. Lee, C. P.; Mandal, N.; Dyson, M. R. Proceedings of the National Academy of Sciences, Vol. 90, Issue 15, p. 7149-7152 https://doi.org/10.1073/pnas.90.15.7149	journal	August 1993
Compositions and Methods for Making Selenocysteine Containing Polypeptides Soll, Dieter; Aldag, Caroline; Hohn, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140154744 US Patent Application 14/131382; 20140154744	patent-application	June 2014
A simple structural feature is a major determinant of the identity of a transfer RNA Hou, Ya-Ming; Schimmel, Paul Nature, Vol. 333, Issue 6169, p. 140-145 https://doi.org/10.1038/333140a0	journal	May 1988
High-velocity microprojectiles for delivering nucleic acids into living cells Klein, T. M.; Wolf, E. D.; Wu, R. Nature, Vol. 327, Issue 6117, p. 70-73 https://doi.org/10.1038/327070a0	journal	May 1987
High-level secretion of correctly processed recombinant human interleukin-1β in Kluyveromyces lactis Fleer, Reinhard; Chen, Xin Jie; Amellal, Nacima Gene, Vol. 107, Issue 2, p. 285-295 https://doi.org/10.1016/0378-1119(91)90329-A	journal	November 1991
Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA^Cys Zhang, Chun-Mei; Liu, Cuiping; Slater, Simon Nature Structural & Molecular Biology, Vol. 15, Issue 5, p. 507-514 https://doi.org/10.1038/nsmb.1423	journal	April 2008
Recoding the Genetic Code with Selenocysteine Bröcker, Markus J.; Ho, Joanne M. L.; Church, George M. Angewandte Chemie International Edition, Vol. 53, Issue 1, p. 319-323 https://doi.org/10.1002/anie.201308584	journal	November 2013
IMG/M 4 version of the integrated metagenome comparative analysis system Markowitz, Victor M.; Chen, I-Min A.; Chu, Ken Nucleic Acids Research, Vol. 42, Issue D1, p. D568-D573 https://doi.org/10.1093/nar/gkt919	journal	October 2013
Conversion of aminoacylation specificity from tRNA^Tyr to tRNA^Ser in vitro Himeno, Hyouta; Hasegawa, Tsunemi; Ueda, Takuya Nucleic Acids Research, Vol. 18, Issue 23, p. 6815-6819 https://doi.org/10.1093/nar/18.23.6815	journal	January 1990
A dual-specificity aminoacyl-tRNA synthetase in the deep-rooted eukaryote Giardia lamblia Bunjun, S.; Stathopoulos, C.; Graham, D. Proceedings of the National Academy of Sciences, Vol. 97, Issue 24, p. 12997-13002 https://doi.org/10.1073/pnas.230444397	journal	November 2000
Purification and characterization of IL6-PE^4e, a recombinant fusion of interleukin 6 with Pseudomonas exotoxin Kreitman, Robert J.; Pastan, Ira Bioconjugate Chemistry, Vol. 4, Issue 6, p. 581-585 https://doi.org/10.1021/bc00024a025	journal	November 1993
Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl-tRNA Mizutani, Takaharu; Tachibana, Yoshio FEBS Letters, Vol. 207, Issue 1, p. 162-166 https://doi.org/10.1016/0014-5793(86)80032-1	journal	October 1986
Pyrrolysyl-tRNA synthetase–tRNA^Pyl structure reveals the molecular basis of orthogonality Nozawa, Kayo; O’Donoghue, Patrick; Gundllapalli, Sarath Nature, Vol. 457, Issue 7233, p. 1163-1167 https://doi.org/10.1038/nature07611	journal	December 2008
Improved tools for biological sequence comparison. Pearson, W. R.; Lipman, D. J. Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448 https://doi.org/10.1073/pnas.85.8.2444	journal	April 1988
Expansion of Noncanonical V-Arm-Containing tRNAs in Eukaryotes Hamashima, Kiyofumi; Tomita, Masaru; Kanai, Akio Molecular Biology and Evolution, Vol. 33, Issue 2, p. 530-540 https://doi.org/10.1093/molbev/msv253	journal	November 2015
The biosynthesis of cysteine and homocysteine in Methanococcus jannaschii White, Robert H. Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1624, Issue 1-3, p. 46-53 https://doi.org/10.1016/j.bbagen.2003.09.005	journal	December 2003
Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code Bain, J. D.; Switzer, Christopher; Chamberlin, Richard Nature, Vol. 356, Issue 6369, p. 537-539 https://doi.org/10.1038/356537a0	journal	April 1992
Catalytic Mechanism of Sep-tRNA:Cys-tRNA Synthase: Sulfur Transfer Is Mediated By Disulfide And Persulfide Liu, Yuchen; Dos Santos, Patricia C.; Zhu, Xiang Journal of Biological Chemistry, Vol. 287, Issue 8, p. 5426-5433 https://doi.org/10.1074/jbc.M111.313700	journal	December 2011
Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens: Implications for the Evolution of a Genetic Code Expansion Cassette Borrel, Guillaume; Gaci, Nadia; Peyret, Pierre Archaea, Vol. 2014, p. 1-11 https://doi.org/10.1155/2014/374146	journal	January 2014
The selective tRNA aminoacylation mechanism based on a single G•U pair Naganuma, Masahiro; Sekine, Shun-ichi; Chong, Yeeting Esther Nature, Vol. 510, Issue 7506, p. 507-511 https://doi.org/10.1038/nature13440	journal	June 2014
Compilation of tRNA sequences and sequences of tRNA genes Sprinzl, Mathias; Horn, Carsten; Brown, Melissa Nucleic Acids Research, Vol. 26, Issue 1, p. 148-153 https://doi.org/10.1093/nar/26.1.148	journal	January 1998
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA Cooley, L.; Appel, B.; Soll, D. Proceedings of the National Academy of Sciences, Vol. 79, Issue 21, p. 6475-6479 https://doi.org/10.1073/pnas.79.21.6475	journal	November 1982
Phosphoserine Aminotransferase, the Second Step-Catalyzing Enzyme for Serine Biosynthesis Basurko, Marie-Jose; Marche, Michele; Darriet, Monique IUBMB: Life, Vol. 48, Issue 5 https://doi.org/10.1080/152165499306630	journal	November 1999
Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter Masson, Jean-Michel; Miller, Jeffrey H. Gene, Vol. 47, Issue 2-3, p. 179-183 https://doi.org/10.1016/0378-1119(86)90061-2	journal	January 1986
Phosphoserine Aminotransferase, the Second Step-Catalyzing Enzyme for Serine Biosynthesis Basurko, Marie-Jose; Marche, Michele; Darriet, Monique IUBMB Life, Vol. 48, Issue 5, p. 525-529 https://doi.org/10.1080/713803557	journal	November 1999
Direct charging of tRNA_CUA with pyrrolysine in vitro and in vivo Blight, Sherry K.; Larue, Ross C.; Mahapatra, Anirban Nature, Vol. 431, Issue 7006, p. 333-335 https://doi.org/10.1038/nature02895	journal	August 2004
A general method applicable to the search for similarities in the amino acid sequence of two proteins Needleman, Saul B.; Wunsch, Christian D. Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453 https://doi.org/10.1016/0022-2836(70)90057-4	journal	March 1970
Photoreactive Bicyclic Amino Acids as Substrates for Mutant Escherichia coli Phenylalanyl-tRNA Synthetases Bentin, Thomas; Hamzavi, Ramin; Salomonsson, Johan Journal of Biological Chemistry, Vol. 279, Issue 19, p. 19839-19845 https://doi.org/10.1074/jbc.M401278200	journal	March 2004
Toward understanding phosphoseryl-tRNA^Cys formation: The crystal structure of Methanococcus maripaludis phosphoseryl-tRNA synthetase Kamtekar, S.; Hohn, M. J.; Park, H.-S. Proceedings of the National Academy of Sciences, Vol. 104, Issue 8, p. 2620-2625 https://doi.org/10.1073/pnas.0611504104	journal	February 2007
Computational identification of the selenocysteine tRNA (tRNA^Sec) in genomes Santesmasses, Didac; Mariotti, Marco; Guigó, Roderic PLOS Computational Biology, Vol. 13, Issue 2, Article No. e1005383 https://doi.org/10.1371/journal.pcbi.1005383	journal	February 2017
The long extra arms of human tRNA^(Ser)Sec and tRNA^Ser function as major identity elements for serylation in an orientation-dependent, but not sequence-specific manner Wu, Xin-Qi; Gross, Hans J. Nucleic Acids Research, Vol. 21, Issue 24, p. 5589-5594 https://doi.org/10.1093/nar/21.24.5589	journal	January 1993
Thermodynamic and Kinetic Framework of Selenocysteyl-tRNA^Sec Recognition by Elongation Factor SelB Paleskava, Alena; Konevega, Andrey L.; Rodnina, Marina V. Journal of Biological Chemistry, Vol. 285, Issue 5, p. 3014-3020 https://doi.org/10.1074/jbc.M109.081380	journal	November 2009
Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286 Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V. Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887 https://doi.org/10.1021/ja971927a	journal	November 1997
Mutagenesis-based definitions and probes of residue burial in proteins Bajaj, K.; Chakrabarti, P.; Varadarajan, R. Proceedings of the National Academy of Sciences, Vol. 102, Issue 45, p. 16221-16226 https://doi.org/10.1073/pnas.0505089102	journal	October 2005
A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1 Mino, Koshiki; Ishikawa, Kazuhiko FEBS Letters, Vol. 551, Issue 1-3, p. 133-138 https://doi.org/10.1016/S0014-5793(03)00913-X	journal	August 2003
Expression of genes transferred into monocot and dicot plant cells by electroporation. Fromm, M.; Taylor, L. P.; Walbot, V. Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828 https://doi.org/10.1073/pnas.82.17.5824	journal	September 1985
Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNA Sec kinase Sherrer, R. Lynn; Ho, Joanne M. L.; Söll, Dieter Nucleic Acids Research, Vol. 36, Issue 6, p. 1871-1880 https://doi.org/10.1093/nar/gkn036	journal	February 2008
Compositions and Methods for Making Selenocysteine Containing Polypeptides Soll, Dieter; Aldag, Caroline; Hohn, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170002347 US Patent Application 15/202194; 20170002347	patent-application	January 2017
Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis Stathopoulos, C.; Kim, W.; Li, T. Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14292-14297 https://doi.org/10.1073/pnas.201540498	journal	November 2001
A semi-synthetic organism with an expanded genetic alphabet Malyshev, Denis A.; Dhami, Kirandeep; Lavergne, Thomas Nature, Vol. 509, Issue 7500, p. 385-388 https://doi.org/10.1038/nature13314	journal	May 2014
The role of tightly bound ATP in Escherichia coli tRNA nucleotidyltransferase Tomari, Yukihide; Suzuki, Tsutomu; Watanabe, Kimitsuna Genes to Cells, Vol. 5, Issue 9, p. 689-698 https://doi.org/10.1046/j.1365-2443.2000.00360.x	journal	September 2000
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production Daly, Rachel; Hearn, Milton T. W. Journal of Molecular Recognition, Vol. 18, Issue 2, p. 119-138 https://doi.org/10.1002/jmr.687	journal	January 2005
Stationary-phase expression and aminoacylation of a transfer-RNA-like small RNA Ataide, Sandro F.; Jester, Brian C.; Devine, Kevin M. EMBO reports, Vol. 6, Issue 8, p. 742-747 https://doi.org/10.1038/sj.embor.7400474	journal	August 2005
Selenoproteins—What unique properties can arise with selenocysteine in place of cysteine? Arnér, Elias S. J. Experimental Cell Research, Vol. 316, Issue 8, p. 1296-1303 https://doi.org/10.1016/j.yexcr.2010.02.032	journal	May 2010
The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser) Biou, V.; Yaremchuk, A.; Tukalo, M. Science, Vol. 263, Issue 5152, p. 1404-1410 https://doi.org/10.1126/science.8128220	journal	March 1994
An engineered selenocysteine defines a unique class of antibody derivatives Hofer, T.; Thomas, J. D.; Burke, T. R. Proceedings of the National Academy of Sciences, Vol. 105, Issue 34, p. 12451-12456 https://doi.org/10.1073/pnas.0800800105	journal	August 2008
Design and Evolution of New Catalytic Activity with an Existing Protein Scaffold Park, Hee-Sung; Nam, Sung-Hun; Lee, Jin Kak Science, Vol. 311, Issue 5760, p. 535-538 https://doi.org/10.1126/science.1118953	journal	January 2006
Artificial lantipeptides from in vitro translations Seebeck, Florian P.; Ricardo, Alonso; Szostak, Jack W. Chemical Communications, Vol. 47, Issue 21, p. 6141-6143 https://doi.org/10.1039/c0cc05663d	journal	January 2011
Adding New Chemistries to the Genetic Code Liu, Chang C.; Schultz, Peter G. Annual Review of Biochemistry, Vol. 79, Issue 1, p. 413-444 https://doi.org/10.1146/annurev.biochem.052308.105824	journal	June 2010
Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins Lacourciere, G. M.; Levine, R. L.; Stadtman, T. C. Proceedings of the National Academy of Sciences, Vol. 99, Issue 14, p. 9150-9153 https://doi.org/10.1073/pnas.142291199	journal	June 2002
Selenocysteine in proteins—properties and biotechnological use Johansson, Linda; Gafvelin, Guro; Arnér, Elias S.J. Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1726, Issue 1, p. 1-13 https://doi.org/10.1016/j.bbagen.2005.05.010	journal	October 2005
UGA is an additional glycine codon in uncultured SR1 bacteria from the human microbiota Campbell, J. H.; O'Donoghue, P.; Campbell, A. G. Proceedings of the National Academy of Sciences, Vol. 110, Issue 14, p. 5540-5545 https://doi.org/10.1073/pnas.1303090110	journal	March 2013
Selenite is a substrate for calf thymus thioredoxin reductase and thioredoxin and elicits a large non-stoichiometric oxidation of NADPH in the presence of oxygen Kumar, Sushil; Bjornstedt, Mikael; Holmgren, Arne European Journal of Biochemistry, Vol. 207, Issue 2, p. 435-439 https://doi.org/10.1111/j.1432-1033.1992.tb17068.x	journal	July 1992
Efficiency of Mammalian Selenocysteine Incorporation Mehta, Anupama; Rebsch, Cheryl M.; Kinzy, Scott A. Journal of Biological Chemistry, Vol. 279, Issue 36, p. 37852-37859 https://doi.org/10.1074/jbc.M404639200	journal	June 2004
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine Polycarpo, C.; Ambrogelly, A.; Berube, A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 34, p. 12450-12454 https://doi.org/10.1073/pnas.0405362101	journal	August 2004
The many levels of control on bacterial selenoprotein synthesis Yoshizawa, Satoko; Böck, August Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1790, Issue 11, p. 1404-1414 https://doi.org/10.1016/j.bbagen.2009.03.010	journal	November 2009
The Human SepSecS-tRNA^Sec Complex Reveals the Mechanism of Selenocysteine Formation Palioura, S.; Sherrer, R. L.; Steitz, T. A. Science, Vol. 325, Issue 5938, p. 321-325 https://doi.org/10.1126/science.1173755	journal	July 2009
Applications and statistics for multiple high-scoring segments in molecular sequences. Karlin, S.; Altschul, S. F. Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877 https://doi.org/10.1073/pnas.90.12.5873	journal	June 1993
Reconstitution of Selenocysteine Incorporation Reveals Intrinsic Regulation by SECIS Elements Gupta, Nirupama; DeMong, Louise W.; Banda, Sowmya Journal of Molecular Biology, Vol. 425, Issue 14, p. 2415-2422 https://doi.org/10.1016/j.jmb.2013.04.016	journal	July 2013
Wobble decoding by the Escherichia coli selenocysteine insertion machinery Xu, J.; Croitoru, V.; Rutishauser, D. Nucleic Acids Research, Vol. 41, Issue 21, p. 9800-9811 https://doi.org/10.1093/nar/gkt764	journal	August 2013
Transfer RNAs That Insert Selenocysteine Carlson, Bradley A.; Hatfield, Dolph L. Protein Sensors and Reactive Oxygen Species - Part A: Selenoproteins and Thioredoxin, p. 24-39 https://doi.org/10.1016/S0076-6879(02)47005-X	book	January 2002
Synthesis of Cysteinyl-tRNACys by A Prolyl-tRNA Synthetase Zhang, Chun-Mei; Hou, Ya-Ming RNA Biology, Vol. 1, Issue 1 https://doi.org/10.4161/rna.1.1.910	journal	May 2004
An aminoacyl tRNA synthetase whose sequence fits into neither of the two known classes Fàbrega, Carme; Farrow, Mark A.; Mukhopadhyay, Biswarup Nature, Vol. 411, Issue 6833, p. 110-114 https://doi.org/10.1038/35075121	journal	May 2001
Expanding the Genetic Code of Escherichia coli Wang, L.; Brock, A.; Herberich, B. Science, Vol. 292, Issue 5516, p. 498-500 https://doi.org/10.1126/science.1060077	journal	April 2001
Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif Shchedrina, V. A.; Novoselov, S. V.; Malinouski, M. Yu. Proceedings of the National Academy of Sciences, Vol. 104, Issue 35, p. 13919-13924 https://doi.org/10.1073/pnas.0703448104	journal	August 2007
Cysteinyl-tRNA^Cys Formation in Methanocaldococcus jannaschii: the Mechanism Is Still Unknown Ruan, B.; Nakano, H.; Tanaka, M. Journal of Bacteriology, Vol. 186, Issue 1, p. 8-14 https://doi.org/10.1128/JB.186.1.8-14.2004	journal	December 2003
Shotgun Proteomics of Methanococcus jannaschii and Insights into Methanogenesis Zhu, Wenhong; Reich, Claudia I.; Olsen, Gary J. Journal of Proteome Research, Vol. 3, Issue 3, p. 538-548 https://doi.org/10.1021/pr034109s	journal	June 2004
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea Fukunaga, Ryuya; Yokoyama, Shigeyuki Nature Structural & Molecular Biology, Vol. 14, Issue 4, p. 272-279 https://doi.org/10.1038/nsmb1219	journal	March 2007

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