The Next Generation of Split-Fluorescent Proteins and What They Can Do for You
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
A symmetrically split fluorescent protein (split-FP) systems provide unique advantages over conventional fluorophores as well as whole fluorescent proteins for cellular imaging and biochemical assays. In this presentation I will discuss some of these advantages, as well as new optimizations and applications for existing LANL split-FP tools in the emerging field of intrinsically disordered protein (IDP) biology, host-pathogen interactions and materials science. I will also briefly present additions to the LANL split-FP toolbox, such as a new split red fluorescent protein (a vast improvement over the current state of the art) and a new technique to develop multimerization-resistant proteins. Lastly, I will discuss some use-cases for these new materials.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- DOE Contract Number:
- AC52-06NA25396
- OSTI ID:
- 1457316
- Report Number(s):
- LA-UR-18-25520
- Country of Publication:
- United States
- Language:
- English
Similar Records
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging
Portable Hyperspectral Multiphoton Excitation Fluorescence Lifetime Microscopy Imager